[ Objective ] This study aimed to identify a cellulase-producing strain selected from tropical rain forestry soils. [ Method ] Morphologic observation and sequence analysis of 18S rDNA were conducted. [Result] A cellu...[ Objective ] This study aimed to identify a cellulase-producing strain selected from tropical rain forestry soils. [ Method ] Morphologic observation and sequence analysis of 18S rDNA were conducted. [Result] A cellulase-preducing strain with high activity was obtained, and morphology of the strain was highly similar to that of Trichoderma reesei. Results of sequence analysis show that the 18S rDNA sequence shares 99% homology with Hypocreajecorina. [ Conclusion] The isolated cellulase-producing strain belongs to Trichoderma reesei.展开更多
基金Supported by 973 Program ( 2010CB126600 )863 Key Project ( 2007AA021307)+3 种基金National Natural Science Foundation ( 31000029 )Special Fund for Basic Research Operating Expenses of Central Nonprofit Research Institutions of Chinese Academy of Tropical Agriculture (ITBBKF1010,ITBBZD0951,ITTBB110103)Hainan Provincial Introduction Integrated Special Fund ( YJJC2011004 )Hainan Provincial Graduate Innovation Fund( Hyb2011-4)
文摘[ Objective ] This study aimed to identify a cellulase-producing strain selected from tropical rain forestry soils. [ Method ] Morphologic observation and sequence analysis of 18S rDNA were conducted. [Result] A cellulase-preducing strain with high activity was obtained, and morphology of the strain was highly similar to that of Trichoderma reesei. Results of sequence analysis show that the 18S rDNA sequence shares 99% homology with Hypocreajecorina. [ Conclusion] The isolated cellulase-producing strain belongs to Trichoderma reesei.