Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus(S.aureus)is of importance in the fi eld of clinical research and practical application.Herein,we report a magnetic relaxation switching(MRS)...Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus(S.aureus)is of importance in the fi eld of clinical research and practical application.Herein,we report a magnetic relaxation switching(MRS)-based strategy for detecting S.aureus in complex samples via the assistance of signal amplifi cation system of liposome.In this design,vancomycin(Van)modifi ed 200 nm magnetic beads(MB_(200)-Van)and biotinylated immunoglobulin(Biotin-IgG)were employed as capture probes for S.aureus which following labelled by biotinylated liposomes loading with glucose molecule(Biotinliposome@Glu,BLGs)based on the affi nity of biotin with streptavidin.The released glucose molecules combining with glucose oxidase generated Fe^(3+),causing transverse relaxation time(T_(2))signal change.The combination of dual recognition of S.aureus,liposome assisted signal amplifi cation,and the MRS-based assay achieved direct and specifi c detection of S.aureus in blood and abscess samples.The as-proposed method for S.aureus assay in this study shows potential application for infectious diagnosis in clinical.展开更多
基金supported by the National Natural Science Foundation of China(22174116,21974110)Chongqing Science Funds for Distinguished Young Scientists(cstc2021jcyjjqx0024)the Innovation Research Group at higher Education Institutions in Chongqing,Chongqing Education Committee(CXQT21006)
文摘Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus(S.aureus)is of importance in the fi eld of clinical research and practical application.Herein,we report a magnetic relaxation switching(MRS)-based strategy for detecting S.aureus in complex samples via the assistance of signal amplifi cation system of liposome.In this design,vancomycin(Van)modifi ed 200 nm magnetic beads(MB_(200)-Van)and biotinylated immunoglobulin(Biotin-IgG)were employed as capture probes for S.aureus which following labelled by biotinylated liposomes loading with glucose molecule(Biotinliposome@Glu,BLGs)based on the affi nity of biotin with streptavidin.The released glucose molecules combining with glucose oxidase generated Fe^(3+),causing transverse relaxation time(T_(2))signal change.The combination of dual recognition of S.aureus,liposome assisted signal amplifi cation,and the MRS-based assay achieved direct and specifi c detection of S.aureus in blood and abscess samples.The as-proposed method for S.aureus assay in this study shows potential application for infectious diagnosis in clinical.
