Multiomics approaches to uncover endometrial receptivity in embryo implantation:a mini-review

Successful implantation requires concerted interactions during the apposition,adhesion,and invasion of the embryo into a receptive endometrium.However,the embryo implantation rate for assisted reproduction remains low despite the transfer of good quality embryos.Changes in endometrial transcriptomics,proteomics,lipidomics,and even microbiota all play important roles in embryo implantation.Specifically,the expression of steroid hormone-regulated adhesive and anti-adhesive molecules during the embryo implantation window is becoming an area of increasingly intense research.This review(a)summarizes the different molecules expressed in the receptive endometrium and(b)proposes the use of surface protein markers to predict pregnancy outcomes from assisted reproduction.

An update on hormonal regulation of early embryo-fallopian tube interactions: insights for improvements of assisted reproductive technology outcomes

Human fallopian tubes(FTs)play important roles in the whole reproductive process.FTs are the site for the final maturation of gametes,fertilization,transport of preimplantation embryos,and early embryonic development before entering the uterus.All these functions are critical in the establishment of a successful pregnancy.When the embryo is in transit through the FT,the FT microenvironment changes through endocrine,paracrine,autocrine,and juxtacrine mechanisms which involve various hormones and bioactive substances mainly from the neighboring cells.Therefore,the interactions between the embryo and the FT may be the earliest embryo-maternal communications that are essential for the establishment of pregnancy.This review critically discusses the evidence of various hormonal and bioactive mediated control of the microenvironment in the FTs during the earliest embryo-maternal communications.Moreover,we highlight existing gaps in the knowledge and the importance of exploring more of the physiology of FTs which will hold the key to producing high-quality embryos for patients seekingin vitro fertilization(IVF)treatments.

The novel male meiosis recombination regulator coordinates the progression of meiosis prophase Ⅰ

Meiosis is a specialized cell division for producing haploid gametes in sexually reproducing organisms.In this study,we have independently identified a novel meiosis protein male meiosis recombination regulator(MAMERR)/4930432 K21 Rik and showed that it is indispensable for meiosis prophase I progression in male mice.Using super-resolution structured illumination microscopy,we found that MAMERR functions at the same double-strand breaks as the replication protein A and meiosis-specific with OB domains/spermatogenesis associated 22 complex.We generated a Mamerr-deficient mouse model by deleting exons 3 e6 and found that most of Mamerrà/àspermatocytes were arrested at pachynema and failed to progress to diplonema,although they exhibited almost intact synapsis and progression to the pachytene stage along with XY body formation.Further mechanistic studies revealed that the recruitment of DMC1/RAD51 and heat shock factor 2 ebinding protein in Mamerrà/àspermatocytes was only mildly impaired with a partial reduction in double-strand break repair,whereas a substantial reduction in ubiquitination on the autosomal axes and on the XY body appeared as a major phenotype in Mamerrà/àspermatocytes.We propose that MAMERR may participate in meiotic prophase I progression by regulating the ubiquitination of key meiotic proteins on autosomes and XY chromosomes,and in the absence of MAMERR,the repressed ubiquitination of key meiotic proteins leads to pachytene arrest and cell death.