Cerebellar Degeneration-related Antigen 1 Is Ubiquitously Expressed in Human Epidermis and Dermis

Cerebellar degeneration-related antigen 1(CDR1)was described to be expressed in the nervous system and in different types of cancer tissues.In the present study,we demonstrate that CDR1 is in addition ubiquitously expressed in human epidermis,dermis and isolated skin cells.Both CDR1 mRNA and protein were detected in human skin-derived mast cells,melanocytes,fibroblasts and keratinocytes,suggesting that CDR1 does not have a neuron-specific function.

定量检测绿色荧光蛋白-轻链蛋白3转基因小鼠角质形成细胞自噬水平

目的 探索一种高通量定量检测细胞自噬小体数量的方法。方法 将绿色荧光蛋白？轻链蛋白3（GFP？LC3）转基因小鼠的角质形成细胞分为对照组（不接受照射或饥饿处理）、饥饿组（给予饥饿处理）、20 J/cm^2长波紫外线（UVA）照射组和40 J/cm^2 UVA照射组。处理后6 h，将细胞固定后在共聚焦显微镜下采集图片。用ImageJ软件编辑宏指令对细胞内GFP？LC3绿色荧光点及细胞进行自动计数，并比较不同组间自噬水平。结果 利用ImageJ软件编辑的宏指令可以成功识别并计数荧光图片中的自噬小体。在测试计算机上分析一张420万像素的图片仅需不到0.6 s。饥饿组、20 J/cm^2 UVA照射组和40 J/cm^2 UVA照射组平均自噬小体数量高于对照组，未加胃抑素A时各组间比较，F = 5.01，P 〈 0.05；加入胃抑素A时各组间比较，F = 20.05，P 〈 0.05。该方法可以成功区分饥饿组、UVA照射组和对照组的自噬水平差异。结论 成功为自噬研究提供了一种新的高通量定量检测方法，该方法能够快速准确地检测细胞自噬荧光点。