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Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae’ 被引量:2
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作者 Yuntao Dai eugenia winston +1 位作者 James C.Correll Yulin Jia 《The Crop Journal》 SCIE CAS 2014年第1期1-9,共9页
The AVR-Pita1 gene,from the Chinese isolate O-137 of Magnaporthe oryzae,is an effector that determines the efficacy of the Pi-ta rice blast resistance gene.In the present study,the avirulence function of AVR-Pita1 was... The AVR-Pita1 gene,from the Chinese isolate O-137 of Magnaporthe oryzae,is an effector that determines the efficacy of the Pi-ta rice blast resistance gene.In the present study,the avirulence function of AVR-Pita1 was induced by transformation of field isolates(TM2,ZN19,B2 and B8)that originally were collected from the U.S.and are virulent on Pi-ta-carrying rice cultivars.The presence of AVR-Pita1 from O-137 in independent transformants was detected by PCR using AVR-Pita1 specific primers and verified by DNA sequencing and Southern blot analysis using the AVR-Pita1 coding region as a probe.The results of pathogenicity assays showed that the AVR-Pita1-transformed isolates were not able to infect rice cultivars Katy and Drew carrying Pi-ta.Control isolates that were transformed with inserts lacking the AVR-Pita1gene remained virulent.Our findings demonstrate that AVR-Pita1 can be used to induce novel gene-specific blast resistance in nature. 展开更多
关键词 AVIRULENCE gene AVR-Pita1 Pi-ta RICE BLAST resistance
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