三元基序家族蛋白7(tripartite motif-containing protein 7,TRIM7)作为E3泛素连接酶TRIM家族的成员,在免疫调控、代谢等生理过程中发挥重要调控作用。此外,TRIM7的异常表达与肝细胞癌(hepatocellular carcinoma,HCC)的发生发展密切相...三元基序家族蛋白7(tripartite motif-containing protein 7,TRIM7)作为E3泛素连接酶TRIM家族的成员,在免疫调控、代谢等生理过程中发挥重要调控作用。此外,TRIM7的异常表达与肝细胞癌(hepatocellular carcinoma,HCC)的发生发展密切相关并呈现出复杂的调控作用,但其在HCC中的表达调控机制尚不清楚。本研究首先利用多种在线数据库分析发现,TRIM7在HCC中高表达,并且TRIM7高表达的肝细胞癌患者预后较差;利用UCSC、JASPAR数据库分析预测TRIM7基因启动子区的转录因子结合位点。结果显示,TRIM7启动子上含有4个特异性蛋白1(specificity protein 1,SP1)转录因子结合位点。本研究利用双荧光素酶报告实验、ChIP-PCR方法检测发现,SP1通过直接结合在TRIM7启动子上的SP1结合位点,从而正调控TRIM7启动子驱动的转录活性。RT-qPCR、Western印迹检测结果进一步显示,过表达SP1在mRNA和蛋白质水平均上调TRIM7基因的表达(P<0.01),且利用SP1抑制剂Mithramycin A处理能够逆转SP1对TRIM7基因表达的调控作用(P<0.01)。总之,本研究初步揭示了TRIM7在肝细胞癌中高表达的调控机制,为深入研究该基因功能以及早期诊断、靶向治疗提供重要的理论依据。展开更多
Maintaining caprock integrity is prerequisite for hydrocarbon accumulation. And gypsolyte caprock integrity is mainly affected by fracturing. Composition, damage behavior and mechanical strength of Paleocene Artashi F...Maintaining caprock integrity is prerequisite for hydrocarbon accumulation. And gypsolyte caprock integrity is mainly affected by fracturing. Composition, damage behavior and mechanical strength of Paleocene Artashi Formation gypsolyte rock that seals significant petroleum in the Kashi Sag of Tarim Basin had been revealed via X-ray diffraction and triaxial compression test. The results indicate the Artashi Formation can be lithologically divided into the lower and upper lithologic members. The lower member comprises gypsum as the dominant mineral, and the cohesion and friction coefficient are 8 MPa and 0.315, respectively. Similarly, the upper lithologic member consists mainly of anhydrite at the cohesion and coefficient of internal friction values of 18 MPa and 0.296. Given that the failure criterion and brittle-ductile transition factors during burial, the sealing integrity of Artashi Formation can be quantized for seven different stages. The reservoirs at the bottom of Artashi Formation caprock buried from 2285 m to 3301 m are expected to be the most favorable exploration target in the Kashi Sag.展开更多
文摘三元基序家族蛋白7(tripartite motif-containing protein 7,TRIM7)作为E3泛素连接酶TRIM家族的成员,在免疫调控、代谢等生理过程中发挥重要调控作用。此外,TRIM7的异常表达与肝细胞癌(hepatocellular carcinoma,HCC)的发生发展密切相关并呈现出复杂的调控作用,但其在HCC中的表达调控机制尚不清楚。本研究首先利用多种在线数据库分析发现,TRIM7在HCC中高表达,并且TRIM7高表达的肝细胞癌患者预后较差;利用UCSC、JASPAR数据库分析预测TRIM7基因启动子区的转录因子结合位点。结果显示,TRIM7启动子上含有4个特异性蛋白1(specificity protein 1,SP1)转录因子结合位点。本研究利用双荧光素酶报告实验、ChIP-PCR方法检测发现,SP1通过直接结合在TRIM7启动子上的SP1结合位点,从而正调控TRIM7启动子驱动的转录活性。RT-qPCR、Western印迹检测结果进一步显示,过表达SP1在mRNA和蛋白质水平均上调TRIM7基因的表达(P<0.01),且利用SP1抑制剂Mithramycin A处理能够逆转SP1对TRIM7基因表达的调控作用(P<0.01)。总之,本研究初步揭示了TRIM7在肝细胞癌中高表达的调控机制,为深入研究该基因功能以及早期诊断、靶向治疗提供重要的理论依据。
基金Project(41672121)supported by the National Natural Science Foundation of ChinaProject(D1438)supported by the China Geological Survey
文摘Maintaining caprock integrity is prerequisite for hydrocarbon accumulation. And gypsolyte caprock integrity is mainly affected by fracturing. Composition, damage behavior and mechanical strength of Paleocene Artashi Formation gypsolyte rock that seals significant petroleum in the Kashi Sag of Tarim Basin had been revealed via X-ray diffraction and triaxial compression test. The results indicate the Artashi Formation can be lithologically divided into the lower and upper lithologic members. The lower member comprises gypsum as the dominant mineral, and the cohesion and friction coefficient are 8 MPa and 0.315, respectively. Similarly, the upper lithologic member consists mainly of anhydrite at the cohesion and coefficient of internal friction values of 18 MPa and 0.296. Given that the failure criterion and brittle-ductile transition factors during burial, the sealing integrity of Artashi Formation can be quantized for seven different stages. The reservoirs at the bottom of Artashi Formation caprock buried from 2285 m to 3301 m are expected to be the most favorable exploration target in the Kashi Sag.
