为了最大限度地发挥脉冲式井底交变流场提速工具的性能,采用数值模拟的方法对与提速工具配套的PDC钻头进行研究,模拟钻井液通过其产生的井底交变流场,研究了钻头刀翼类型、刀翼数量和喷嘴数量对钻井液井底冲击力和井底清岩效果的影响,...为了最大限度地发挥脉冲式井底交变流场提速工具的性能,采用数值模拟的方法对与提速工具配套的PDC钻头进行研究,模拟钻井液通过其产生的井底交变流场,研究了钻头刀翼类型、刀翼数量和喷嘴数量对钻井液井底冲击力和井底清岩效果的影响,并对钻头结构进行了优选。研究结果表明:螺旋刀翼、7喷嘴、6刀翼钻头产生的脉冲射流具有较高的平均静压冲击力和井底冲击力振幅;6直刀翼6喷嘴钻头对应的井底动压冲击力波动最大,达到7.0 kN以上,5直刀翼7喷嘴钻头对应的井底静压冲击力波动最大,为2.8 k N;5直刀翼5喷嘴钻头的清岩效果最好,其产生的井底最小岩屑质量浓度和平均岩屑质量浓度最低,且井底岩屑质量浓度的波动幅值最大,达到1.6 kg/m^3;对于软到中硬地层,配合使用脉冲式井底交变流场提速工具,选择5直刀翼5喷嘴PDC钻头清岩和携岩效果较好;对于硬地层,选用6直刀翼6喷嘴PDC钻头提速效果较好。所得结论可为脉冲射流钻井提速工具配套PDC钻头结构设计及应用提供参考。展开更多
Carrimycin(CA),sanctioned by China’s National Medical Products Administration(NMPA)in 2019 for treating acute bronchitis and sinusitis,has recently been observed to exhibit multifaceted biological activities,encompas...Carrimycin(CA),sanctioned by China’s National Medical Products Administration(NMPA)in 2019 for treating acute bronchitis and sinusitis,has recently been observed to exhibit multifaceted biological activities,encompassing anti-inflammatory,antiviral,and anti-tumor properties.Despite these applications,its efficacy in sepsis treatment remains unexplored.This study introduces a novel function of CA,demonstrating its capacity to mitigate sepsis induced by lipopolysaccharide(LPS)and cecal ligation and puncture(CLP)in mice models.Our research employed in vitro assays,real-time quantitative polymerase chain reaction(RT-qPCR),and RNA-seq analysis to establish that CA significantly reduces the levels of pro-inflammatory cytokines,namely tumor necrosis factor-alpha(TNF-α),interleukin 1 beta(IL-1β),and interleukin 6(IL-6),in response to LPS stimulation.Additionally,Western blotting and immunofluorescence assays revealed that CA impedes Nuclear Factor Kappa B(NF-κB)activation in LPS-stimulated RAW264.7 cells.Complementing these findings,in vivo experiments demonstrated that CA effectively alleviates LPS-and CLP-triggered organ inflammation in C57BL/6 mice.Further insights were gained through 16S sequencing,highlighting CA’s pivotal role in enhancing gut microbiota diversity and modulating metabolic pathways,particularly by augmenting the production of short-chain fatty acids in mice subjected to CLP.Notably,a comparative analysis revealed that CA’s anti-inflammatory efficacy surpasses that of equivalent doses of aspirin(ASP)and TIENAM.Collectively,these findings suggest that CA exhibits significant therapeutic potential in sepsis treatment.This discovery provides a foundational theoretical basis for the clinical application of CA in sepsis management.展开更多
文摘为了最大限度地发挥脉冲式井底交变流场提速工具的性能,采用数值模拟的方法对与提速工具配套的PDC钻头进行研究,模拟钻井液通过其产生的井底交变流场,研究了钻头刀翼类型、刀翼数量和喷嘴数量对钻井液井底冲击力和井底清岩效果的影响,并对钻头结构进行了优选。研究结果表明:螺旋刀翼、7喷嘴、6刀翼钻头产生的脉冲射流具有较高的平均静压冲击力和井底冲击力振幅;6直刀翼6喷嘴钻头对应的井底动压冲击力波动最大,达到7.0 kN以上,5直刀翼7喷嘴钻头对应的井底静压冲击力波动最大,为2.8 k N;5直刀翼5喷嘴钻头的清岩效果最好,其产生的井底最小岩屑质量浓度和平均岩屑质量浓度最低,且井底岩屑质量浓度的波动幅值最大,达到1.6 kg/m^3;对于软到中硬地层,配合使用脉冲式井底交变流场提速工具,选择5直刀翼5喷嘴PDC钻头清岩和携岩效果较好;对于硬地层,选用6直刀翼6喷嘴PDC钻头提速效果较好。所得结论可为脉冲射流钻井提速工具配套PDC钻头结构设计及应用提供参考。
基金supported by the Excellent Young Scholars Cultivation Project of Fujian Medical University Union Hospital in China(No.2022XH031)the National Natural Science Foundation of China(No.82203439)the Natural Science Foundation of Fujian Province(No.2022J01263).
文摘Carrimycin(CA),sanctioned by China’s National Medical Products Administration(NMPA)in 2019 for treating acute bronchitis and sinusitis,has recently been observed to exhibit multifaceted biological activities,encompassing anti-inflammatory,antiviral,and anti-tumor properties.Despite these applications,its efficacy in sepsis treatment remains unexplored.This study introduces a novel function of CA,demonstrating its capacity to mitigate sepsis induced by lipopolysaccharide(LPS)and cecal ligation and puncture(CLP)in mice models.Our research employed in vitro assays,real-time quantitative polymerase chain reaction(RT-qPCR),and RNA-seq analysis to establish that CA significantly reduces the levels of pro-inflammatory cytokines,namely tumor necrosis factor-alpha(TNF-α),interleukin 1 beta(IL-1β),and interleukin 6(IL-6),in response to LPS stimulation.Additionally,Western blotting and immunofluorescence assays revealed that CA impedes Nuclear Factor Kappa B(NF-κB)activation in LPS-stimulated RAW264.7 cells.Complementing these findings,in vivo experiments demonstrated that CA effectively alleviates LPS-and CLP-triggered organ inflammation in C57BL/6 mice.Further insights were gained through 16S sequencing,highlighting CA’s pivotal role in enhancing gut microbiota diversity and modulating metabolic pathways,particularly by augmenting the production of short-chain fatty acids in mice subjected to CLP.Notably,a comparative analysis revealed that CA’s anti-inflammatory efficacy surpasses that of equivalent doses of aspirin(ASP)and TIENAM.Collectively,these findings suggest that CA exhibits significant therapeutic potential in sepsis treatment.This discovery provides a foundational theoretical basis for the clinical application of CA in sepsis management.
