Mycophenolic acid derivatives from cultures of the mushroom Laetiporus sulphureu

AIM: To investigate the chemical constituents of the cultures of Laetiporus sulphureus(Bull.) Murrill. METHOD: Compounds were isolated and purified by various chromatographic techniques. The structure of the new compound was determined by interpretation of MS and 1D-, 2D-NMR spectroscopic data, while the known compounds were identified by comparison of their data with those reported. RESULTS: Three mycophenolic acid derivatives, 6-((2E, 6E)-3, 7-dimethyldeca-2, 6-dienyl)-7-hydroxy-5-methoxy-4-methylphtanlan-1-one(1), 6-((2E, 6E)-3, 7, 11-trimethyldedoca-2, 6, 10-trienyl)-5, 7-dihydroxy-4-methylphtanlan-1-one(2), and 6-((2E, 6E)-3, 7, 11-trimethyldedoca-2, 6, 10-trienyl)-7-hydroxy-5-methoxy-4-methylphtanlan-1-one(3) were isolated. CONCLUSION: Among them, compound 1 was new, and compound 2 exhibited moderate cytotoxicity against HL-60, SMMC-7721, A-549, and MCF-7 cells, with IC50 values of 39.1, 31.1, 27.4, and 35.7 μmol·L-1, respectively.

42例流产组织、257例羊水样本和59例脐带血样本全染色体SNP基因芯片分析

目的对流产组织、羊水样本和脐带血样本进行全染色体SNP基因芯片分析,以明确SNP基因芯片在这些样本类型中的检测意义。方法结合常规染色体G显带技术和Cyto-12 SNP基因芯片对收集到的检材实施检测,严格参照检测说明和实验实施流程。结果 47.62%的流产组织、11.28%的羊水样本和11.86%的脐带血样本检出基因拷贝数变异,家系分析结果也证明了部分拷贝数变异具有遗传性。结论基因芯片检测的分辨率较传统核型高,适用于较难实施细胞培养的检测样本,或者有明显症状但常规核型分析未检出异常的样本。目前基因芯片无法分析染色体的结构变异,因此在产前诊断时在完成基因芯片检测的同时,常规核型分析也需要同步实施。