A Novel High Nitrogen Nickel-free Coronary Stents System: Evaluation in a Porcine Model

Objective To study the safety of the novel high nitrogen nickel-free austenitic stainless steel bare metal stents （BMS） in a recognized porcine coronary model and to select a better grid structure of it. Methods Three types of stents were randomly implanted in different coronary arteries of the same pig： 316L stainless steel BMS （316L-BMS） （n=12）, novel high nitrogen nickel-free stents Grid A （NF-A-BMS） （n=12） and novel high nitrogen nickel-free stents Grid B （NF-B-BMS） （n=12）. In total, eighteen animals underwent successful random placement of 36 oversized stents in the coronary arteries. Coronary angiography was performed after 36 d of stents implantation. Nine animals were respectively sacrificed after 14 d and 36 d for histomorphologic analysis. 〈br〉 Results Quantitative coronary angiography （QCA） showed similar luminal loss （LL） in the three groups：（0.21±0.17） mm for 316L-BMS, （0.16±0.12） mm for NF-A-BMS, （0.24±0.15） mm for NF-B-BMS （P=0.05）. Histomorphomeric analysis after 15 d and 36 d revealed that there was also no significant difference among the three groups in neointimal area （NA） with similar injury scores respectively. High magnification histomorphologic examination showed similar inflammation scores in the three groups, but NF-A-BMS group had poorer endothelialization scores compared with NF-B-BMS group, 2.00±0.63 vs. 2.83±0.41 （P=0.015） at 15 d, which also could be proved by the scanning electron microscope. However, the difference could not been observed at 36 d. Conclusion The novel NF-BMS showed similar safety as 316L-BMS during the short-term study. NF-B-BMS had better endothelialization than NF-A-BMS and this may owe to the specific strut units.

华支睾吸虫Severin与HBV-X共同作用对肝细胞和肝癌细胞凋亡和周期影响的相关性研究

目的研究华支睾吸虫Severin蛋白(Csseverin)与HBV-X蛋白(HBX蛋白)共同作用对肝细胞和肝癌细胞凋亡和周期的影响。方法构建表达HBX的稳转肝细胞和肝癌细胞株,经诱导凋亡后用纯化Severin蛋白孵育,检测Severin蛋白对细胞凋亡和周期影响,染色后用流式细胞术检测细胞各期凋亡比例和处于不同周期细胞比例。结果随Csseverin浓度升高,稳转HBX的肝细胞株凋亡比例显著降低,由(24.13±0.68)%降低到(10.72±0.29)%;细胞增殖期比例增加,由15.43%增加到27.72%,而空载质粒对照组无明显变化。随Csseverin浓度升高,稳转HBX的肝癌细胞株凋亡比例显著降低,由(27.75±1.21)%降低到(3.91±0.75)%;细胞增殖期比例增加,由31.96%增加到38.04%。结论Csseverin蛋白与HBV-X共同作用有抑制肝癌细胞凋亡,促进细胞增殖的作用。

原发性胃淋巴上皮瘤样癌的临床及免疫学特征分析

目的比较原发性胃淋巴上皮瘤样癌(gastric lymphoepithelioma-like carcinoma, GLELC)和胃腺癌的临床特征,分析GLELC组织免疫学指标变化。方法原发性GLELC患者18例为GLELC组,同期诊治胃腺癌患者28例为胃腺癌组。比较2组临床资料及手术切除组织中程序性死亡受体-1(programmed cell death-1, PD-1)、程序性死亡受体配体-1(programmed cell death ligand-1, PD-L1)表达情况;比较GLELC组癌组织CD3^(+)、CD4^(+)、CD8^(+)、CD20^(+)细胞比率。结果 GLELC组TNM分期Ⅰ~Ⅱ期比率(77.78%)高于胃腺癌组(35.71%)(P<0.05),Ⅱ、Ⅲ期者肿瘤直径[(5.00±2.10)cm、(7.50±0.91)cm]大于胃腺癌组[(2.80±1.30)、(5.46±2.89)cm](P<0.05),淋巴结转移比率(38.89%)低于胃腺癌组(71.43%)(P<0.05),PD-L1阳性表达率(88.89%)高于胃腺癌组(21.43%)(P<0.05),PD-1阳性表达率(22.22%)与胃腺癌组(17.86%)比较差异无统计学意义(P>0.05)。GLELC组癌组织中CD3^(+)T淋巴细胞比率[(76.39±4.13)%]高于CD20^(+)B淋巴细胞比率[(18.06±3.49)%](t=45.762,P<0.001),CD8^(+)T淋巴细胞比率[48.89±6.54)%]高于CD4^(+)T淋巴细胞比率[(27.50±3.54)%](t=12.202,P<0.001)。结论与胃腺癌相比,原发性GLELC肿瘤分期较低,肿瘤直径较大,淋巴结转移较少,癌组织PD-L1呈高表达,且淋巴细胞浸润以CD3^(+)、CD8^(+)T淋巴细胞为主。