目的:基于网络药理学探索桑贝止嗽散治疗新型冠状病毒肺炎(Corona Virus Disease 2019,COVID-19)(简称新冠肺炎)的药理机制。方法:通过中药网络药理学平台(traditional Chinese medicine systems pharmacology database and analysis pl...目的:基于网络药理学探索桑贝止嗽散治疗新型冠状病毒肺炎(Corona Virus Disease 2019,COVID-19)(简称新冠肺炎)的药理机制。方法:通过中药网络药理学平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索桑贝止嗽散中桑白皮、浙贝母、百部、紫菀四味核心药物的主要活性成分及其对应的靶标基因,同时通过Gene Cards数据库获得新冠肺炎相关靶点,将两者进行映射,获得交集靶点作为桑贝止嗽散治疗新冠肺炎的预测靶点,利用Cytoscape软件、STRING和DAVID 6.8等数据库构建蛋白互作(protein protein interaction,PPI)网络,并进行基因本体(geneontology,GO)富集分析及基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路分析。结果:桑贝止嗽散4味核心药物作用于新冠肺炎的活性成分有68个,相关靶点87个,关键活性化合物8个,关键靶基因17个;GO分析显示,桑贝止嗽散治疗新冠肺炎影响的生物过程主要有脂多糖介导的信号通路,蛋白信号转导,缺氧反应等15个;KEGG通路富集显示桑贝止嗽散治疗新冠肺炎的通路主要有:PI3K-Akt信号通路,钙离子信号通路,T细胞受体信号转导,TNF信号通路等5条。结论:桑贝止嗽散可能通过以上相关通路作用于TNF、VEGFA等关键靶点,影响蛋白表达、信号通路传导等生物过程,发挥治疗新冠肺炎的作用,为实验研究提供一定的理论参考依据。展开更多
Desktop calibration of automatic transmission(AT) is a method which can reduce cost, enhance efficiency and shorten the development periods of a vehicle effectively. We primary introduced the principle and approach of...Desktop calibration of automatic transmission(AT) is a method which can reduce cost, enhance efficiency and shorten the development periods of a vehicle effectively. We primary introduced the principle and approach of desktop calibration of AT based on the condition of coupling characteristics between engine and torque converter and obtained right point exactly. It is shown to agree with experimental measurements reasonably well. It was used in different applications abroad based on AT technology and achieved a good performance of the vehicle compared with traditional AT technology which primary focuses on the drivability, performance and fuel consumption.展开更多
DMRT基因家族是一个与性别决定有关的基因家族,其编码的转录因子DMRTs蛋白(除DMRT8外)均具有一个DM(double-sex and mab-3)结构域,并通过该结构域与DNA结合而发挥调控作用。目前研究表明,DMRT基因家族在性器官发育(如性别分化、性腺发...DMRT基因家族是一个与性别决定有关的基因家族,其编码的转录因子DMRTs蛋白(除DMRT8外)均具有一个DM(double-sex and mab-3)结构域,并通过该结构域与DNA结合而发挥调控作用。目前研究表明,DMRT基因家族在性器官发育(如性别分化、性腺发育、生殖细胞发育等)与性外器官发育(如肌肉软骨发育、神经系统发育等)中均具有重要作用;同时也有学者提出DMRTs可能参与肿瘤(如前列腺癌、乳腺癌、肺癌等)的发生和发展。本文综述了以人与小鼠为主的哺乳动物DMRTs/Dmrts的结构、在性器官发育与性外器官发育中的重要功能及其与肿瘤的关系的研究进展,提示DMRT基因家族在多器官发育及多种肿瘤发生和发展及治疗等过程中可能发挥作用,能够作为深入研究肿瘤发病机制与治疗策略的候选基因。展开更多
文摘目的:基于网络药理学探索桑贝止嗽散治疗新型冠状病毒肺炎(Corona Virus Disease 2019,COVID-19)(简称新冠肺炎)的药理机制。方法:通过中药网络药理学平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索桑贝止嗽散中桑白皮、浙贝母、百部、紫菀四味核心药物的主要活性成分及其对应的靶标基因,同时通过Gene Cards数据库获得新冠肺炎相关靶点,将两者进行映射,获得交集靶点作为桑贝止嗽散治疗新冠肺炎的预测靶点,利用Cytoscape软件、STRING和DAVID 6.8等数据库构建蛋白互作(protein protein interaction,PPI)网络,并进行基因本体(geneontology,GO)富集分析及基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路分析。结果:桑贝止嗽散4味核心药物作用于新冠肺炎的活性成分有68个,相关靶点87个,关键活性化合物8个,关键靶基因17个;GO分析显示,桑贝止嗽散治疗新冠肺炎影响的生物过程主要有脂多糖介导的信号通路,蛋白信号转导,缺氧反应等15个;KEGG通路富集显示桑贝止嗽散治疗新冠肺炎的通路主要有:PI3K-Akt信号通路,钙离子信号通路,T细胞受体信号转导,TNF信号通路等5条。结论:桑贝止嗽散可能通过以上相关通路作用于TNF、VEGFA等关键靶点,影响蛋白表达、信号通路传导等生物过程,发挥治疗新冠肺炎的作用,为实验研究提供一定的理论参考依据。
文摘Desktop calibration of automatic transmission(AT) is a method which can reduce cost, enhance efficiency and shorten the development periods of a vehicle effectively. We primary introduced the principle and approach of desktop calibration of AT based on the condition of coupling characteristics between engine and torque converter and obtained right point exactly. It is shown to agree with experimental measurements reasonably well. It was used in different applications abroad based on AT technology and achieved a good performance of the vehicle compared with traditional AT technology which primary focuses on the drivability, performance and fuel consumption.
基金supported by the Science and Technology Department of Henan Province,China(No.222102310186,162102310523)the College Students’Innovation and Entrepreneurship Training Program Projects of Zhengzhou University,China(No.2021cxcy290)。
文摘DMRT基因家族是一个与性别决定有关的基因家族,其编码的转录因子DMRTs蛋白(除DMRT8外)均具有一个DM(double-sex and mab-3)结构域,并通过该结构域与DNA结合而发挥调控作用。目前研究表明,DMRT基因家族在性器官发育(如性别分化、性腺发育、生殖细胞发育等)与性外器官发育(如肌肉软骨发育、神经系统发育等)中均具有重要作用;同时也有学者提出DMRTs可能参与肿瘤(如前列腺癌、乳腺癌、肺癌等)的发生和发展。本文综述了以人与小鼠为主的哺乳动物DMRTs/Dmrts的结构、在性器官发育与性外器官发育中的重要功能及其与肿瘤的关系的研究进展,提示DMRT基因家族在多器官发育及多种肿瘤发生和发展及治疗等过程中可能发挥作用,能够作为深入研究肿瘤发病机制与治疗策略的候选基因。
