目的基于网络药理学分析石菖蒲主要成分治疗神经退行性疾病(neurodegenerative diseases,ND)的作用靶点和机制。方法利用TCMSP(traditional Chinese medicine systems pharmacology database and analysis platform)数据库检索和既往文...目的基于网络药理学分析石菖蒲主要成分治疗神经退行性疾病(neurodegenerative diseases,ND)的作用靶点和机制。方法利用TCMSP(traditional Chinese medicine systems pharmacology database and analysis platform)数据库检索和既往文献证明得到石菖蒲的主要有效成分。在GeneCards,OMIM(Online Mendelian Inheritance in Man),TTD(Therapeutic Target Database)数据库检索ND的相关靶点,并用Uniprot数据库进行标准化。构建ND和石菖蒲有效成分的共有靶点韦恩图和PPI网络图,进行GO(Gene ontology)注释和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,展示石菖蒲有效成分在治疗ND过程中疾病-靶点的关系。结果石菖蒲和ND的共有靶点总计为41个,关键靶点主要有FOS、ACHE、SLC6A4、ADRA1B、ADRA2A、MAOB、PTGS2、ADRA1A等。这些靶点涉及G蛋白偶联的胺受体活性、肾上腺素受体活性、儿茶酚胺结合、类固醇激素受体活性、神经递质受体活性、突触后神经递质受体活性等与突触间神经递质及其受体密切相关的生物过程、钙信号通路、cGMP-PKG信号通路和唾液分泌通路等信号通路。结论石菖蒲的主要有效成分可能通过对FOS、ACHE、SLC6A4、ADRA1B、MAOB等蛋白及相关通路的影响进而对神经神经递质受体或突触的损伤修复发挥治疗ND的作用,这为石菖蒲有效成分治疗ND的作用机制提供了新方向。展开更多
Peiai 64S is a photoperiod- and thermo- sensitive genic male sterile (PTSGMS) line of rice, which is male sterile at long day/high temperature and partial fertile at short day/low temperature. A cDNA array representin...Peiai 64S is a photoperiod- and thermo- sensitive genic male sterile (PTSGMS) line of rice, which is male sterile at long day/high temperature and partial fertile at short day/low temperature. A cDNA array representing 3328 unique rice genes was used to profile the gene expression patterns in the young panicles of Peiai 64S under these two condi- tions. The statistical data showed that up to 14.60% of genes exhibited up-or down-regulated expressions in the plants at long day/high temperature compared with plants at short day/low temperature. Only four genes were up-regulated while 482 genes down-regulated. Real-time PCR with all the up-regulated and 9 randomly selected down-regulated genes confirmed the differential expressions detected by the array, indicating that the constructed cDNA array is reliable. These differently expressed genes participated in almost all cell biological responses. Analysis of up- and down-regulated genes revealed distinctive changes between the mRNA abundances of MMK1 and MMK2, both of which are analogs ofMAPK, and significant down-regulation of several transcription factors. It was suggested that changes that occurred in the MAPK signal transduction path- way might disturb the transcription control necessary for morphogenesis of pollens and corresponding phys- iological functions.展开更多
The present paper describes multiple splicing types of OsRIX4,an RAD21 homolog in rice. A type of alternative splicing (AS),distinctive from all five previously known splicing types,was identified in which interior se...The present paper describes multiple splicing types of OsRIX4,an RAD21 homolog in rice. A type of alternative splicing (AS),distinctive from all five previously known splicing types,was identified in which interior sequences of a constitutive exon could be spliced. Translation of the transcript produced with this AS type was demonstrated at the protein level. Expression of multiple transcripts was organ specific. The expression abundance of transcripts,OsRIX4-4 and OsRIX4-5,was positively correlated with fertility in rice. The splicing type identified in the present study provided the means to further un-derstand and define different mRNA splicing types in plants and suggested that post-transcription processing of mRNA and its regulation mechanism are complex.展开更多
文摘目的基于网络药理学分析石菖蒲主要成分治疗神经退行性疾病(neurodegenerative diseases,ND)的作用靶点和机制。方法利用TCMSP(traditional Chinese medicine systems pharmacology database and analysis platform)数据库检索和既往文献证明得到石菖蒲的主要有效成分。在GeneCards,OMIM(Online Mendelian Inheritance in Man),TTD(Therapeutic Target Database)数据库检索ND的相关靶点,并用Uniprot数据库进行标准化。构建ND和石菖蒲有效成分的共有靶点韦恩图和PPI网络图,进行GO(Gene ontology)注释和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,展示石菖蒲有效成分在治疗ND过程中疾病-靶点的关系。结果石菖蒲和ND的共有靶点总计为41个,关键靶点主要有FOS、ACHE、SLC6A4、ADRA1B、ADRA2A、MAOB、PTGS2、ADRA1A等。这些靶点涉及G蛋白偶联的胺受体活性、肾上腺素受体活性、儿茶酚胺结合、类固醇激素受体活性、神经递质受体活性、突触后神经递质受体活性等与突触间神经递质及其受体密切相关的生物过程、钙信号通路、cGMP-PKG信号通路和唾液分泌通路等信号通路。结论石菖蒲的主要有效成分可能通过对FOS、ACHE、SLC6A4、ADRA1B、MAOB等蛋白及相关通路的影响进而对神经神经递质受体或突触的损伤修复发挥治疗ND的作用,这为石菖蒲有效成分治疗ND的作用机制提供了新方向。
文摘Peiai 64S is a photoperiod- and thermo- sensitive genic male sterile (PTSGMS) line of rice, which is male sterile at long day/high temperature and partial fertile at short day/low temperature. A cDNA array representing 3328 unique rice genes was used to profile the gene expression patterns in the young panicles of Peiai 64S under these two condi- tions. The statistical data showed that up to 14.60% of genes exhibited up-or down-regulated expressions in the plants at long day/high temperature compared with plants at short day/low temperature. Only four genes were up-regulated while 482 genes down-regulated. Real-time PCR with all the up-regulated and 9 randomly selected down-regulated genes confirmed the differential expressions detected by the array, indicating that the constructed cDNA array is reliable. These differently expressed genes participated in almost all cell biological responses. Analysis of up- and down-regulated genes revealed distinctive changes between the mRNA abundances of MMK1 and MMK2, both of which are analogs ofMAPK, and significant down-regulation of several transcription factors. It was suggested that changes that occurred in the MAPK signal transduction path- way might disturb the transcription control necessary for morphogenesis of pollens and corresponding phys- iological functions.
基金Supported by the National Natural Science Foundation of China (Grant No. 30170592)Zhejiang Provincial Science and Technology Project (Grant No. 2005C22002)
文摘The present paper describes multiple splicing types of OsRIX4,an RAD21 homolog in rice. A type of alternative splicing (AS),distinctive from all five previously known splicing types,was identified in which interior sequences of a constitutive exon could be spliced. Translation of the transcript produced with this AS type was demonstrated at the protein level. Expression of multiple transcripts was organ specific. The expression abundance of transcripts,OsRIX4-4 and OsRIX4-5,was positively correlated with fertility in rice. The splicing type identified in the present study provided the means to further un-derstand and define different mRNA splicing types in plants and suggested that post-transcription processing of mRNA and its regulation mechanism are complex.