Seaweed Complex Preparation （SCP） is a clinical traditional Chinese medicine preparation which is composed of seven traditional Chinese herbs, and it has been used for treatment of lung cancer, liver cancer and dige...Seaweed Complex Preparation （SCP） is a clinical traditional Chinese medicine preparation which is composed of seven traditional Chinese herbs, and it has been used for treatment of lung cancer, liver cancer and digestive cancer. However, little infor- mation is available about the pharmacodynamic basis. The antitumor, immunomodulatory and free radical scavenging effects of SCP were evaluated in this study. Transplanted tumor in vivo method was used to determine the antitumor effect. The effects on spleno- cyte proliferation and phagocytosis of macrophages in tumor-bearing mice were measured by the MTT method and the phagocytizing cock red blood cell （CRBC） method respectively. The scavenging activities of SCP on DPPH and hydroxyl radicals in vitro were investigated. It was found that the medium-dose and high-dose of SCP could significantly inhibit the growth of transplanted hepatic tumor of murine hepatocarcinoma cell line H22, and promote proliferation of splenocytes and phagocytosis of macrophages. SCP possessed noticeable scavenging activities on DPPH and hydroxyl radicals. The antiturnor effects of SCP might be achieved by im- proving immune system and scavenging free radicals, which is in accordance with the viewpoint of traditional Chinese medicine in promoting the body resistance and eliminating pathogenic factors for cancer treatment.展开更多
To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of sponge Gelliodes gracilis was constructed. The average size of DNA inserts in the libra...To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of sponge Gelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper disc assaying. Two clones displayed the antibacterial activity against Micrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the host E. coli DNA and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts.展开更多
This article deals with extraction and purification of deguelin,which is a main rotenoid occurred in the root of Derris trifoliate Lour.Deguelin has gained much attention of scientists because of its potential ability...This article deals with extraction and purification of deguelin,which is a main rotenoid occurred in the root of Derris trifoliate Lour.Deguelin has gained much attention of scientists because of its potential ability to inhibit cancer cell proliferation.The dried root powder was extracted with ethanol by ultrasonic-assisted extraction for 30 min,and then filtrated and concentrated to give pasty concentrate.The concentrate was loaded onto silica gel column chromatography subsequently;and the chemical was eluted using a binary solvent mixture of petroleum ether(60-90ºC)-ethyl acetate(4:1,v/v).All tentative identification was carried out by high performance liquid chromatography(HPLC).Most crystal of deguelin was obtained after the fractions mainly containing deguelin were pooled and placed in the dark at 4ºC for three days.With recrystallization from carbon tetrachloride for three times,the purity of deguelin crystal was 99.15%and the yield was 0.55%of the dried weight of D.trifoliate Lour root.展开更多
基金supported by the National Natural Science Foundation of China(No.30572314)the Basic Research Program of Science and Technology,Ministry of Science and Technology of China(No.2007FY210500)+1 种基金the Program of Chinese Offshore Investigation and Assessment,State Oceanic Administration of China(Nos.908-01-ST12,908-02-05-04)Science and Technology Program of Shandong Province,China(No.03BS109)
文摘Seaweed Complex Preparation （SCP） is a clinical traditional Chinese medicine preparation which is composed of seven traditional Chinese herbs, and it has been used for treatment of lung cancer, liver cancer and digestive cancer. However, little infor- mation is available about the pharmacodynamic basis. The antitumor, immunomodulatory and free radical scavenging effects of SCP were evaluated in this study. Transplanted tumor in vivo method was used to determine the antitumor effect. The effects on spleno- cyte proliferation and phagocytosis of macrophages in tumor-bearing mice were measured by the MTT method and the phagocytizing cock red blood cell （CRBC） method respectively. The scavenging activities of SCP on DPPH and hydroxyl radicals in vitro were investigated. It was found that the medium-dose and high-dose of SCP could significantly inhibit the growth of transplanted hepatic tumor of murine hepatocarcinoma cell line H22, and promote proliferation of splenocytes and phagocytosis of macrophages. SCP possessed noticeable scavenging activities on DPPH and hydroxyl radicals. The antiturnor effects of SCP might be achieved by im- proving immune system and scavenging free radicals, which is in accordance with the viewpoint of traditional Chinese medicine in promoting the body resistance and eliminating pathogenic factors for cancer treatment.
基金This work was supported by High Tech R&D Program of China(Grant Nos.2002AA628130 and 2003AA624020)the National Natural Science Foundation of China(30171102)+2 种基金the Fund for Cheung Kong Scholar from the Cheung Kong Scholar Program of Ministry of Education of Chinathe Fund from the Natural Science Foundation of Shandong Province(No.Z2001C01)the High Tech R&D Program of Shandong Province(No.0121100107).The authors would like to thank Professor Li Jinhe of Institute of 0ceanology,Chinese Academy of Sciences,for the identification of the sponge.
文摘To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of sponge Gelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper disc assaying. Two clones displayed the antibacterial activity against Micrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the host E. coli DNA and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts.
文摘This article deals with extraction and purification of deguelin,which is a main rotenoid occurred in the root of Derris trifoliate Lour.Deguelin has gained much attention of scientists because of its potential ability to inhibit cancer cell proliferation.The dried root powder was extracted with ethanol by ultrasonic-assisted extraction for 30 min,and then filtrated and concentrated to give pasty concentrate.The concentrate was loaded onto silica gel column chromatography subsequently;and the chemical was eluted using a binary solvent mixture of petroleum ether(60-90ºC)-ethyl acetate(4:1,v/v).All tentative identification was carried out by high performance liquid chromatography(HPLC).Most crystal of deguelin was obtained after the fractions mainly containing deguelin were pooled and placed in the dark at 4ºC for three days.With recrystallization from carbon tetrachloride for three times,the purity of deguelin crystal was 99.15%and the yield was 0.55%of the dried weight of D.trifoliate Lour root.
