[ Objective] To extract non-specific transfer factors （TFs） from goose spleens and to detect the activity of the extracts. [Methodl The non-specific TFs were extracted crudely from goose spleens using the dialysis m...[ Objective] To extract non-specific transfer factors （TFs） from goose spleens and to detect the activity of the extracts. [Methodl The non-specific TFs were extracted crudely from goose spleens using the dialysis method, and the effects of the extracts on immunity were studied. [ Result] The extracts of goose TFs were transparent, light yellow and non-toxic. The extracts were at pH 6.72 and did not contain higher molecular weight proteins. The concentration of minor polypeptides was 23.78 ug/ml. The results of immunization test showed that the level of antibodies was higher in the geese inoculated with the inactivated vaccine against infections serositis and the extracts of TFs than in the control geese injected with normal saline. In addition, the level of antibodies was significantly higher in the geese only inoculated with inactivated vaccine than in the control geese. The results showed that the extracts of TFs from goose spleens could enhance immunity to a certain extent. [ Conclusion] The study pro- vides a theoretical basis and technical reference for further researches on the biological activity of non-specific TFs from goose spleens as well as for application of the extracts.展开更多
基金funded by the Innovation Fund Project for College Students of Rongchang Campus of Southwest University,Chongqing Basic Research program ( 2008BB5243)the Fundamental Research Funds for the Central Universities( XDJK2010C093)
文摘[ Objective] To extract non-specific transfer factors （TFs） from goose spleens and to detect the activity of the extracts. [Methodl The non-specific TFs were extracted crudely from goose spleens using the dialysis method, and the effects of the extracts on immunity were studied. [ Result] The extracts of goose TFs were transparent, light yellow and non-toxic. The extracts were at pH 6.72 and did not contain higher molecular weight proteins. The concentration of minor polypeptides was 23.78 ug/ml. The results of immunization test showed that the level of antibodies was higher in the geese inoculated with the inactivated vaccine against infections serositis and the extracts of TFs than in the control geese injected with normal saline. In addition, the level of antibodies was significantly higher in the geese only inoculated with inactivated vaccine than in the control geese. The results showed that the extracts of TFs from goose spleens could enhance immunity to a certain extent. [ Conclusion] The study pro- vides a theoretical basis and technical reference for further researches on the biological activity of non-specific TFs from goose spleens as well as for application of the extracts.
