Gray mold caused by Botrytis cinerea is a major disease of greenhouse cucumbers and its control is largely dependent on the succinate dehydrogenase inhibitor boscalid.In this study,gray mold-infected cucumbers were co...Gray mold caused by Botrytis cinerea is a major disease of greenhouse cucumbers and its control is largely dependent on the succinate dehydrogenase inhibitor boscalid.In this study,gray mold-infected cucumbers were collected from greenhouses located in 11 towns in which boscalid had been used,and 163 B.cinerea strains were isolated by single-spore method.The EC50 value effective concentration that could inhibit conidia germination by 50%compared with the control for sensitive isolates ranged from 0.045μg/mL to 50.148μg/mL in the assays.Additionally,101 isolates were resistant to boscalid,and the resistance frequency ranged from 33.33%to 86.67%in these towns.Most of the resistant isolates showed a low or moderately resistant phenotype,with the resistance frequency of moderately resistant isolates being as high as 80%.The SdhB gene harbored a single mutation corresponding to His272.The fitness evaluation showed that the hyphal growth,sporulation,germinability and pathogenicity were different among the resistant and sensitive isolates.The results suggested that dual resistance to boscalid in B.cinerea populations could result in failure to control gray mold.展开更多
A total of 96 monoconidial Botrytis cinerea strains were isolated from strawberry field.Physiological characteristics and resistance analysis to boscalid were investigated.Most isolates showed resistant phenotype and ...A total of 96 monoconidial Botrytis cinerea strains were isolated from strawberry field.Physiological characteristics and resistance analysis to boscalid were investigated.Most isolates showed resistant phenotype and the resistance frequency of highly resistant isolates was as high as 45.10%.The effective concentration that inhibited conidia germination by 50% relative to the control values for sensitive isolates ranged from 0.068 to 60.56μg·mL^-1 in conidia germination assays.Compared to the sensitive strains,the boscalid-resistant strains had a significant increase in phenylalanine ammonia lyase(PAL)and peroxidase(POD)activity and more sensitivity to boscalid pressure.Fitness analysis showed statistically significant difference between sensitive and resistance isolates.Sequencing of SdhB gene indicated that a single mutation corresponded to His272.The results suggested that resistance to boscalid in B.cinerea populations could result in failure to control gray mold.These results would enrich the understanding of the resistance mechanism of B.cinerea to boscalid.展开更多
利用稀释涂布法从番茄根际土壤中分离放线菌,并以番茄灰霉菌为靶标,利用对峙培养法和牛津杯法筛选拮抗放线菌,得到一株具有较强抑菌活性的放线菌LA-5.通过培养特征、生理生化特性及基于16S r DNA序列系统进化分析,将菌株LA-5初步鉴定为...利用稀释涂布法从番茄根际土壤中分离放线菌,并以番茄灰霉菌为靶标,利用对峙培养法和牛津杯法筛选拮抗放线菌,得到一株具有较强抑菌活性的放线菌LA-5.通过培养特征、生理生化特性及基于16S r DNA序列系统进化分析,将菌株LA-5初步鉴定为链霉菌.复筛结果显示,LA-5发酵滤液对番茄灰霉菌孢子萌发及菌丝生长均有明显的抑制作用,其中100倍发酵滤液对孢子萌发抑制率和菌丝生长抑制率均在50%以上;受抑制菌落呈白色,气生菌丝萎缩稀疏,菌丝纤细、分支明显减少.离体防效试验显示,菌株LA-5发酵原液对番茄灰霉病防效可达83.4%.该菌株有望开发为防治番茄灰霉病的生防菌株.展开更多
基金Supported by the National Natural Science Foundation of China(31501665)the Application Program of Yuncheng University(CY2018016)the Doctoral Fund of Yuncheng University(YQ-2018012) and Subject Program of Yuncheng University(XK-2018006)~~
文摘Gray mold caused by Botrytis cinerea is a major disease of greenhouse cucumbers and its control is largely dependent on the succinate dehydrogenase inhibitor boscalid.In this study,gray mold-infected cucumbers were collected from greenhouses located in 11 towns in which boscalid had been used,and 163 B.cinerea strains were isolated by single-spore method.The EC50 value effective concentration that could inhibit conidia germination by 50%compared with the control for sensitive isolates ranged from 0.045μg/mL to 50.148μg/mL in the assays.Additionally,101 isolates were resistant to boscalid,and the resistance frequency ranged from 33.33%to 86.67%in these towns.Most of the resistant isolates showed a low or moderately resistant phenotype,with the resistance frequency of moderately resistant isolates being as high as 80%.The SdhB gene harbored a single mutation corresponding to His272.The fitness evaluation showed that the hyphal growth,sporulation,germinability and pathogenicity were different among the resistant and sensitive isolates.The results suggested that dual resistance to boscalid in B.cinerea populations could result in failure to control gray mold.
基金Supported by the National Natural Science Foundation of China(31501665)the Program for the Scientific and Technologal Innovation Programs Higher Education Institutions in Shanxi(2019L0849,2019L0851)+1 种基金the Opening Foundation of Key Laboratory of Environment Change and Resources Use in Beibu Gulf Ministry of Education(Nanning Normal University)Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation(Nanning Normal University)(NNNU-KLOP-K1904)
文摘A total of 96 monoconidial Botrytis cinerea strains were isolated from strawberry field.Physiological characteristics and resistance analysis to boscalid were investigated.Most isolates showed resistant phenotype and the resistance frequency of highly resistant isolates was as high as 45.10%.The effective concentration that inhibited conidia germination by 50% relative to the control values for sensitive isolates ranged from 0.068 to 60.56μg·mL^-1 in conidia germination assays.Compared to the sensitive strains,the boscalid-resistant strains had a significant increase in phenylalanine ammonia lyase(PAL)and peroxidase(POD)activity and more sensitivity to boscalid pressure.Fitness analysis showed statistically significant difference between sensitive and resistance isolates.Sequencing of SdhB gene indicated that a single mutation corresponded to His272.The results suggested that resistance to boscalid in B.cinerea populations could result in failure to control gray mold.These results would enrich the understanding of the resistance mechanism of B.cinerea to boscalid.
文摘利用稀释涂布法从番茄根际土壤中分离放线菌,并以番茄灰霉菌为靶标,利用对峙培养法和牛津杯法筛选拮抗放线菌,得到一株具有较强抑菌活性的放线菌LA-5.通过培养特征、生理生化特性及基于16S r DNA序列系统进化分析,将菌株LA-5初步鉴定为链霉菌.复筛结果显示,LA-5发酵滤液对番茄灰霉菌孢子萌发及菌丝生长均有明显的抑制作用,其中100倍发酵滤液对孢子萌发抑制率和菌丝生长抑制率均在50%以上;受抑制菌落呈白色,气生菌丝萎缩稀疏,菌丝纤细、分支明显减少.离体防效试验显示,菌株LA-5发酵原液对番茄灰霉病防效可达83.4%.该菌株有望开发为防治番茄灰霉病的生防菌株.
