An ultrasensitive electrochemical biosensor was fabricated for electroanalytical determination of ascorbic acid(AA),dopamine(DA)and uric acid(UA)individually and simultaneously based on polypyrrole hollow nanotubes lo...An ultrasensitive electrochemical biosensor was fabricated for electroanalytical determination of ascorbic acid(AA),dopamine(DA)and uric acid(UA)individually and simultaneously based on polypyrrole hollow nanotubes loaded with Au and Fe_(3)O_(4) nanoparticles(NPs)uniformly(PPy@Au-Fe_(3)O_(4)).The PPy@Au-Fe_(3)O_(4) nanotubes were synthesized in one-pot using MoO_(3) nanorods as templates and the polymerization of Py,the formation of Au and Fe_(3)O_(4) NPs and the removel of MoO_(3) templates took place stimultaneously.Electrochemical studies reveal that PPy@Au-Fe_(3)O_(4) modified glassy carbon electrode(GCE)possesses excellent electrocatalytic activities toward the oxidation of AA,DA and UA.Their oxidation peak currents increase linearly in the concentration ranges of 1–2000µmol/L for AA,0.01–25 and 25–300µmol/L for DA and 0.1–300µmol/L for UA.Their detection limit values(S/N=3)were calculated as 0.45,0.0049,and 0.051µmol/L for AA,DA and UA in the individual detection.By changing the concentrations simultaneously,the calibration curves showed linearity to 1000,200,and 200µmol/L with detection limit of 0.39,0.0060,and 0.060µmol/L for AA,DA,and UA,respectively.Finally,the obtained biosensor was successfully applied to the detection of AA,DA,and UA with satisfactory results on actual samples.展开更多
基金supported by the National Natural Science Foundation of China(Nos.22004028,21722501,21803018)the Henan Special Support for High-Level Talents Central Plains Science and Technology Innovation Leading Talents of China(No.204200510006)+1 种基金the Key Project of Science and Technology of Henan Province,China(Nos.202102310139,2121102311071)the Natural Science Foundation of Henan Province,China(No.222300420207).
文摘An ultrasensitive electrochemical biosensor was fabricated for electroanalytical determination of ascorbic acid(AA),dopamine(DA)and uric acid(UA)individually and simultaneously based on polypyrrole hollow nanotubes loaded with Au and Fe_(3)O_(4) nanoparticles(NPs)uniformly(PPy@Au-Fe_(3)O_(4)).The PPy@Au-Fe_(3)O_(4) nanotubes were synthesized in one-pot using MoO_(3) nanorods as templates and the polymerization of Py,the formation of Au and Fe_(3)O_(4) NPs and the removel of MoO_(3) templates took place stimultaneously.Electrochemical studies reveal that PPy@Au-Fe_(3)O_(4) modified glassy carbon electrode(GCE)possesses excellent electrocatalytic activities toward the oxidation of AA,DA and UA.Their oxidation peak currents increase linearly in the concentration ranges of 1–2000µmol/L for AA,0.01–25 and 25–300µmol/L for DA and 0.1–300µmol/L for UA.Their detection limit values(S/N=3)were calculated as 0.45,0.0049,and 0.051µmol/L for AA,DA and UA in the individual detection.By changing the concentrations simultaneously,the calibration curves showed linearity to 1000,200,and 200µmol/L with detection limit of 0.39,0.0060,and 0.060µmol/L for AA,DA,and UA,respectively.Finally,the obtained biosensor was successfully applied to the detection of AA,DA,and UA with satisfactory results on actual samples.
