水稻穗发育是产量形成的基础。从粳稻品种‘武香粳9号’组织培养后代中筛选获得一份幼穗发育异常的突变体pdf1(panicle development failure 1)。突变体pdf1能正常从营养生长向生殖生长转换,穗分化能正常起始,但随后穗轴基部细胞出现程...水稻穗发育是产量形成的基础。从粳稻品种‘武香粳9号’组织培养后代中筛选获得一份幼穗发育异常的突变体pdf1(panicle development failure 1)。突变体pdf1能正常从营养生长向生殖生长转换,穗分化能正常起始,但随后穗轴基部细胞出现程序性死亡,引起穗发育停止。不同生长条件处理试验表明,pdf1突变性状受温度调控。转录组测序显示,差异表达基因主要参与DNA代谢过程、信号转导等生物过程,并定位于质膜和过氧化物酶体,其可能与信号刺激和过氧化物酶体等细胞通路的改变有关。展开更多
Grain shape and size are two key factors that determine rice yield and quality. In the present study, a rice triangular hull mutant (tri1) was obtained from the progeny of japonica rice variety Taipei 309 treated with...Grain shape and size are two key factors that determine rice yield and quality. In the present study, a rice triangular hull mutant (tri1) was obtained from the progeny of japonica rice variety Taipei 309 treated with 60Co γ-rays. Compared to the wild type, the tri1 mutant presents a triangular hull, and exhibits an increase in grain thickness and protein content, but with a slight decrease in plant height and grain weight. Genetic analysis indicated that the mutant phenotype was controlled by a recessive nuclear gene which is stably inherited. Using a map-based cloning strategy, we fine-mapped tri1 to a 47-kb region between the molecular markers CHR0122 and CHR0127 on the long arm of chromosome 1, and showed that it co-segregates with the molecular marker CHR0119. According to the rice genome sequence annotation there are six predicated genes within the mapped region. Sequencing analysis of the mutant and the wild type indicated that there was a deletion of an A nucleotide in exon 3 of the OsMADS32 gene, which could result in a downstream frameshift mutation and premature termination of the predicted polypeptide. Both semi-quantitative and real-time RT-PCR analyses showed that this gene expressed highly in young inflorescences, while expressed at very low levels in other tissues. These results implied that the OsMADS32 gene could be a candidate of TRI1. Taken together, the results of this study lay the foundation for further investigation into the molecular mechanisms regulating rice caryopsis development.展开更多
文摘水稻穗发育是产量形成的基础。从粳稻品种‘武香粳9号’组织培养后代中筛选获得一份幼穗发育异常的突变体pdf1(panicle development failure 1)。突变体pdf1能正常从营养生长向生殖生长转换,穗分化能正常起始,但随后穗轴基部细胞出现程序性死亡,引起穗发育停止。不同生长条件处理试验表明,pdf1突变性状受温度调控。转录组测序显示,差异表达基因主要参与DNA代谢过程、信号转导等生物过程,并定位于质膜和过氧化物酶体,其可能与信号刺激和过氧化物酶体等细胞通路的改变有关。
基金supported by the National Key Basic Research and Development Project (2012CB944803)the National Natural Science Foundation of China (31071383)+1 种基金the Funds for Distinguished Young Scientists of Jiangsu Province (BK2012010)Priority Academic Program Development from Jiangsu Government, China
文摘Grain shape and size are two key factors that determine rice yield and quality. In the present study, a rice triangular hull mutant (tri1) was obtained from the progeny of japonica rice variety Taipei 309 treated with 60Co γ-rays. Compared to the wild type, the tri1 mutant presents a triangular hull, and exhibits an increase in grain thickness and protein content, but with a slight decrease in plant height and grain weight. Genetic analysis indicated that the mutant phenotype was controlled by a recessive nuclear gene which is stably inherited. Using a map-based cloning strategy, we fine-mapped tri1 to a 47-kb region between the molecular markers CHR0122 and CHR0127 on the long arm of chromosome 1, and showed that it co-segregates with the molecular marker CHR0119. According to the rice genome sequence annotation there are six predicated genes within the mapped region. Sequencing analysis of the mutant and the wild type indicated that there was a deletion of an A nucleotide in exon 3 of the OsMADS32 gene, which could result in a downstream frameshift mutation and premature termination of the predicted polypeptide. Both semi-quantitative and real-time RT-PCR analyses showed that this gene expressed highly in young inflorescences, while expressed at very low levels in other tissues. These results implied that the OsMADS32 gene could be a candidate of TRI1. Taken together, the results of this study lay the foundation for further investigation into the molecular mechanisms regulating rice caryopsis development.
