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大黄素通过上调lncRNA MEG3表达并抑制PI3K/AKT信号通路影响喉癌细胞的增殖和凋亡 被引量:5
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作者 冯国泰 李阳松 张玲莉 《中国药师》 CAS 2021年第8期463-467,共5页
目的:探讨大黄素对喉癌M4E细胞增殖和凋亡的影响及作用机制。方法:大黄素作用于M4E细胞24 h,四噻唑蓝(MTT)检测细胞增殖,流式细胞仪检测细胞凋亡,实时定量聚合酶链式反应(RT-qPCR)检测母系表达基因3(MEG3)水平,Western Blot检测细胞周... 目的:探讨大黄素对喉癌M4E细胞增殖和凋亡的影响及作用机制。方法:大黄素作用于M4E细胞24 h,四噻唑蓝(MTT)检测细胞增殖,流式细胞仪检测细胞凋亡,实时定量聚合酶链式反应(RT-qPCR)检测母系表达基因3(MEG3)水平,Western Blot检测细胞周期蛋白D1(CyclinD1)、活化的半胱天冬酶-3(Cleaved-caspase-3)、磷酸化磷酸化磷脂酞肌醇3-激酶p85亚单位(p-PI3Kp85)和磷酸化蛋白激酶B(p-AKT)蛋白。转染MEG3过表达载体至M4E细胞,上述相同方法检测MEG3过表达对M4E细胞增殖、凋亡及相关蛋白表达的影响。结果:与对照组相比,大黄素作用后,M4E细胞增殖抑制率、凋亡率、Cleaved-caspase-3蛋白水平和MEG3水平显著升高(P<0.05),p-PI3Kp85和p-AKT蛋白水平显著降低(P<0.05)。MEG3过表达可提高M4E细胞增殖抑制率、凋亡率和Cleaved-caspase-3蛋白水平(P>0.05),降低CyclinD1蛋白水平(P<0.05)。低表达MEG3逆转了大黄素对M4E细胞增殖、凋亡及p-PI3Kp85和p-AKT蛋白表达的影响(P<0.05)。结论:大黄素可能通过上调MEG3表达并抑制PI3K/AKT信号通路抑制喉癌细胞的增殖,并诱导其凋亡。 展开更多
关键词 喉癌 大黄素 母系表达基因3 细胞增殖 凋亡 PI3K/AKT信号通路
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Study on topology and vortex structure in a diffusion cascade 被引量:1
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作者 ZHANG YongJun WANG HuiShe +1 位作者 XU JianZhong feng guotai 《Science China(Technological Sciences)》 SCIE EI CAS 2009年第8期2305-2315,共11页
To further make clear vortex structures in diffusion cascades so as to help understand the mechanisms of vortex affecting loss production, the emergence, evolution and development of secondary flow vortexes, including... To further make clear vortex structures in diffusion cascades so as to help understand the mechanisms of vortex affecting loss production, the emergence, evolution and development of secondary flow vortexes, including horse shoe vortex, passage vortex and corner vortex and so on, were discussed mainly through using the topological analysis method and numerical calculation. The concept of a three-dimensional dividing surface between the low energy flow zone and the exterior flow zone was presented. The results show that concentrated shed vortex is located outside the dividing surface (in the outer flow zone) and horse shoe vortex, passage vortex and corner vortex are inside the dividing surface (in the low energy flow zone). Dissipation function is used to measure loss production instead of using entropy production. The results about loss analysis indicate that vortex motion directly causes loss production, namely, peak value of loss is generally located around the core of vortex and that maximal loss happens around the dividing surface other than in the low energy flow zone. 展开更多
关键词 DIFFUSION CASCADE TOPOLOGY analysis VORTEX structure DISSIPATION FUNCTION
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