Background Stem cell transplantation has been shown to have beneficial effects on dilated cardiomyopathy. However, mechanism for stem cell homing to cardiac tissue in dilated cardiomyopathy has not yet been elucidated...Background Stem cell transplantation has been shown to have beneficial effects on dilated cardiomyopathy. However, mechanism for stem cell homing to cardiac tissue in dilated cardiomyopathy has not yet been elucidated. Methods Mesenchymal stem cells were obtained from rat bone marrow, expanded in vitro, and labeled with 99mmc. Cardiomyopathy model was induced by doxorubicin in rats. 9ernTC labeled cells were infused into the left ventricles in cardiomyopathy and control rats. Sixteen hours after injection, animals were sacrificed and different tissues were harvested to measure specific radioactivity. By use of real-time polymerase chain reaction and immunohistochemistry, mRNA and protein expressions for stromal-cell-derived factor 1 in cardiac tissue were measured. Results Labeling efficiency of mesenchymal stem cells was （70.0±11.2）%. Sixteen hours after mesenchymal stem cell transplantation, the heart-to-muscle radioactivity ratio was increased significantly in cardiomyopathy hearts as compared to control hearts. Both mRNA and protein expressions of cardiomyopathy hearts as compared with control hearts. Conclusion In dilated cardiomyopathy induced by doxorubicin in heart may induce mesenchymal stem cells home to the heart. stromal-cell-derived factor 1 were up-regulated in up-regulated expression of stromal-cell-derived factor I展开更多
基金This study was supported by the National Natural Science Foundation of China （No. 30900603）, Specialized Research Fund for the Doctoral Program of Higher Education （No. 20093234110008 and No. 20093234120008）, and Project Supported by Department of Health of Jiangsu Province （No. ZX07 200902）
文摘Background Stem cell transplantation has been shown to have beneficial effects on dilated cardiomyopathy. However, mechanism for stem cell homing to cardiac tissue in dilated cardiomyopathy has not yet been elucidated. Methods Mesenchymal stem cells were obtained from rat bone marrow, expanded in vitro, and labeled with 99mmc. Cardiomyopathy model was induced by doxorubicin in rats. 9ernTC labeled cells were infused into the left ventricles in cardiomyopathy and control rats. Sixteen hours after injection, animals were sacrificed and different tissues were harvested to measure specific radioactivity. By use of real-time polymerase chain reaction and immunohistochemistry, mRNA and protein expressions for stromal-cell-derived factor 1 in cardiac tissue were measured. Results Labeling efficiency of mesenchymal stem cells was （70.0±11.2）%. Sixteen hours after mesenchymal stem cell transplantation, the heart-to-muscle radioactivity ratio was increased significantly in cardiomyopathy hearts as compared to control hearts. Both mRNA and protein expressions of cardiomyopathy hearts as compared with control hearts. Conclusion In dilated cardiomyopathy induced by doxorubicin in heart may induce mesenchymal stem cells home to the heart. stromal-cell-derived factor 1 were up-regulated in up-regulated expression of stromal-cell-derived factor I
