A total of 58 morphological characteristics were measured on flower-colour sport and original cultivar according to Disticness, Uniformity, Stability＇ （DUS） Test for New Plant Cultivar of Chrysanthemum published by...A total of 58 morphological characteristics were measured on flower-colour sport and original cultivar according to Disticness, Uniformity, Stability＇ （DUS） Test for New Plant Cultivar of Chrysanthemum published by Ministry of Agriculture of China The results showed that five characteristics such as the surface colour, the back color of ray floret among them were siginificantly different, their MS （mo by AFLP showed that rphological s GS （genetic imilarity） was 91.4%, and MD （morphological difference） was 8.62%. DNA polymorphic analysis similarity） was 98.6%, and GD （genetic polymorphic diversity） was 2.81%. Five distinct bands which may include the flower-colour sport genes or be the molecular marker linkaged flower-color characteristics were amplified.展开更多
Interactions between model target peptide melittin (ME) and Euplotes octocarinatus centrin (EoCen) were investigated by fluorescence spectra, circular dichroism (CD) spectra and native polyacrylamide gel electrophores...Interactions between model target peptide melittin (ME) and Euplotes octocarinatus centrin (EoCen) were investigated by fluorescence spectra, circular dichroism (CD) spectra and native polyacrylamide gel electrophoresis (PAGE). In 0.1 mol/L N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (Hepes) and 150 mmol/L NaCl at pH 7.4, EoCen and isolated short C-terminal domain of EoCen (SC-EoCen) form 1:1 peptide:protein complexes. However, no detectable signal changes can be observed while isolated N-terminal domain of EoCen (N-EoCen) or isolated long C-terminal domain of EoCen (LC-EoCen) was added into solution of ME. The interaction between EoCen and ME is specified exclusively for the short C-terminal domain of EoCen. On the basis of fluorescence titration curves, the conditional binding constants of ME with EoCen and SC-EoCen were calculated to be logKME-EoCen = 6.81±0.33 and log- KME-SC-EoCen = 6.51±0.45, respectively.展开更多
文摘A total of 58 morphological characteristics were measured on flower-colour sport and original cultivar according to Disticness, Uniformity, Stability＇ （DUS） Test for New Plant Cultivar of Chrysanthemum published by Ministry of Agriculture of China The results showed that five characteristics such as the surface colour, the back color of ray floret among them were siginificantly different, their MS （mo by AFLP showed that rphological s GS （genetic imilarity） was 91.4%, and MD （morphological difference） was 8.62%. DNA polymorphic analysis similarity） was 98.6%, and GD （genetic polymorphic diversity） was 2.81%. Five distinct bands which may include the flower-colour sport genes or be the molecular marker linkaged flower-color characteristics were amplified.
基金Supported by the National Natural Science Foundation of China (Grant Nos. 20371031 and 20771068)the Natural Science Foundation of Shanxi Province (Grant No. 2007011024) Young Science Foundation of Shanxi University
文摘Interactions between model target peptide melittin (ME) and Euplotes octocarinatus centrin (EoCen) were investigated by fluorescence spectra, circular dichroism (CD) spectra and native polyacrylamide gel electrophoresis (PAGE). In 0.1 mol/L N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (Hepes) and 150 mmol/L NaCl at pH 7.4, EoCen and isolated short C-terminal domain of EoCen (SC-EoCen) form 1:1 peptide:protein complexes. However, no detectable signal changes can be observed while isolated N-terminal domain of EoCen (N-EoCen) or isolated long C-terminal domain of EoCen (LC-EoCen) was added into solution of ME. The interaction between EoCen and ME is specified exclusively for the short C-terminal domain of EoCen. On the basis of fluorescence titration curves, the conditional binding constants of ME with EoCen and SC-EoCen were calculated to be logKME-EoCen = 6.81±0.33 and log- KME-SC-EoCen = 6.51±0.45, respectively.
