Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, known as carcinogen. The aim of this study was to develop a simple, fast, and accurate method for det...Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, known as carcinogen. The aim of this study was to develop a simple, fast, and accurate method for determining arginine in grape juice with Sakaguchi reaction by separating arginine with strong cation-exchange resins. Parameters were optimized including the concentrations of 8-hydroquinoline and sodium hydrobromite. The color stability lasted for 4 min, which is sufficient to finish the measurement. The method is simple, reproducible and accurate, and can be applied for quick measurement of arginine in grape juice to take necessary measures for controlling the level of ethyl carbamate.展开更多
目的探讨口服葡萄糖耐量试验2小时血糖(OGTT 2 h PBG)对轻度前循环梗死患者颈动脉血流动力学的影响。方法选取2015年10月—2017年8月潍坊市人民医院神经内科收治的轻度前循环梗死患者166例,根据OGTT 2 h PBG分为糖耐量受损组91例(7.8 mm...目的探讨口服葡萄糖耐量试验2小时血糖(OGTT 2 h PBG)对轻度前循环梗死患者颈动脉血流动力学的影响。方法选取2015年10月—2017年8月潍坊市人民医院神经内科收治的轻度前循环梗死患者166例,根据OGTT 2 h PBG分为糖耐量受损组91例(7.8 mmol/L≤OGTT 2 h PBG<11.1 mmol/L)和糖尿病组75例(OGTT 2 h PBG≥11.1 mmol/L);另选取同期体检健康者230例作为对照组。比较3组受试者血脂指标、空腹血糖(FPG)及颈动脉血流动力学指标,OGTT 2 h PBG与轻度前循环梗死患者颈动脉血流动力学指标的相关性分析采用Pearson相关性分析。结果 3组受试者总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)比较,差异无统计学意义(P>0.05);糖尿病组患者低密度脂蛋白胆固醇(LDL-C)和FPG高于糖耐量受损组和对照组,糖耐量受损组患者FPG高于对照组(P<0.05)。糖耐量受损组和糖尿病组患者颈动脉血流剪切力、颈动脉血流峰值剪切力小于对照组,肱动脉介导的血管舒张功能(FMD)劣于对照组(P<0.05);糖尿病组患者颈动脉血流剪切力、颈动脉血流峰值剪切力小于糖耐量受损组,FMD劣于糖耐量受损组(P<0.05)。Pearson相关性分析结果显示,OGTT 2 h PBG与轻度前循环梗死患者颈动脉血流剪切力(r=-0.747)、颈动脉血流峰值剪切力(r=-0.436)及FMD(r=-0.516)呈负相关(P<0.05)。结论 OGTT 2 h PBG升高可导致轻度前循环梗死患者颈动脉血流剪切力降低,影响患者血管舒张功能。展开更多
基金the National Science and Technology Achievements Spreading Programme from the Ministry of Science and Technology of China(2004EC000317)
文摘Arginine in grape juice can be metabolized by wine yeasts and malolactic bacteria to precursors of ethyl carbamate, known as carcinogen. The aim of this study was to develop a simple, fast, and accurate method for determining arginine in grape juice with Sakaguchi reaction by separating arginine with strong cation-exchange resins. Parameters were optimized including the concentrations of 8-hydroquinoline and sodium hydrobromite. The color stability lasted for 4 min, which is sufficient to finish the measurement. The method is simple, reproducible and accurate, and can be applied for quick measurement of arginine in grape juice to take necessary measures for controlling the level of ethyl carbamate.
文摘目的探讨口服葡萄糖耐量试验2小时血糖(OGTT 2 h PBG)对轻度前循环梗死患者颈动脉血流动力学的影响。方法选取2015年10月—2017年8月潍坊市人民医院神经内科收治的轻度前循环梗死患者166例,根据OGTT 2 h PBG分为糖耐量受损组91例(7.8 mmol/L≤OGTT 2 h PBG<11.1 mmol/L)和糖尿病组75例(OGTT 2 h PBG≥11.1 mmol/L);另选取同期体检健康者230例作为对照组。比较3组受试者血脂指标、空腹血糖(FPG)及颈动脉血流动力学指标,OGTT 2 h PBG与轻度前循环梗死患者颈动脉血流动力学指标的相关性分析采用Pearson相关性分析。结果 3组受试者总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)比较,差异无统计学意义(P>0.05);糖尿病组患者低密度脂蛋白胆固醇(LDL-C)和FPG高于糖耐量受损组和对照组,糖耐量受损组患者FPG高于对照组(P<0.05)。糖耐量受损组和糖尿病组患者颈动脉血流剪切力、颈动脉血流峰值剪切力小于对照组,肱动脉介导的血管舒张功能(FMD)劣于对照组(P<0.05);糖尿病组患者颈动脉血流剪切力、颈动脉血流峰值剪切力小于糖耐量受损组,FMD劣于糖耐量受损组(P<0.05)。Pearson相关性分析结果显示,OGTT 2 h PBG与轻度前循环梗死患者颈动脉血流剪切力(r=-0.747)、颈动脉血流峰值剪切力(r=-0.436)及FMD(r=-0.516)呈负相关(P<0.05)。结论 OGTT 2 h PBG升高可导致轻度前循环梗死患者颈动脉血流剪切力降低,影响患者血管舒张功能。
