In this paper,the liquid seed medium of Grifola frondosa was optimized by single factor and orthogonal experiments using the shake flask culture method.The single factor experiments results showed that soybean powder ...In this paper,the liquid seed medium of Grifola frondosa was optimized by single factor and orthogonal experiments using the shake flask culture method.The single factor experiments results showed that soybean powder and maltose were the optimal nitrogen and carbon sources,and MgSO4 was the optimal inorganic salt.The orthogonal experiment results showed that the optimal combination of liquid seed culture medium for Grifola frondosa was:20%potato,0.75%soybean powder,5%maltose,0.1%KH2PO4,0.05%MgSO4,0.001%VB1.Using the optimized medium,shaking culture for 10 d under the inoculum amount of 5%,the temperature of 28℃,and the rotation speed of 150 r/min,the dry weight of mycelium exceeded 1.3228 g/100mL.展开更多
Comparative test of 4 Ganoderma lucidum varieties from different sources showed that the mycelium of Chizhi 1 grew fast with thick and dense hyphae,round and solid cap,and high spore powder yield.Chizhi 1 was proved t...Comparative test of 4 Ganoderma lucidum varieties from different sources showed that the mycelium of Chizhi 1 grew fast with thick and dense hyphae,round and solid cap,and high spore powder yield.Chizhi 1 was proved to be an excellent variety because of its strong resistance and high spore powder yield.Different cultivation materials were chosen and combined to form 3 cultivation formulations.The results showed that Formulation(3),in which basswood was soaked in nutrient solution for 24 h,presented fast mycelial growth and high spore powder output,and therefore was proved to be a high-yield formulation of Ganoderma lucidum spore powder.展开更多
文摘In this paper,the liquid seed medium of Grifola frondosa was optimized by single factor and orthogonal experiments using the shake flask culture method.The single factor experiments results showed that soybean powder and maltose were the optimal nitrogen and carbon sources,and MgSO4 was the optimal inorganic salt.The orthogonal experiment results showed that the optimal combination of liquid seed culture medium for Grifola frondosa was:20%potato,0.75%soybean powder,5%maltose,0.1%KH2PO4,0.05%MgSO4,0.001%VB1.Using the optimized medium,shaking culture for 10 d under the inoculum amount of 5%,the temperature of 28℃,and the rotation speed of 150 r/min,the dry weight of mycelium exceeded 1.3228 g/100mL.
文摘Comparative test of 4 Ganoderma lucidum varieties from different sources showed that the mycelium of Chizhi 1 grew fast with thick and dense hyphae,round and solid cap,and high spore powder yield.Chizhi 1 was proved to be an excellent variety because of its strong resistance and high spore powder yield.Different cultivation materials were chosen and combined to form 3 cultivation formulations.The results showed that Formulation(3),in which basswood was soaked in nutrient solution for 24 h,presented fast mycelial growth and high spore powder output,and therefore was proved to be a high-yield formulation of Ganoderma lucidum spore powder.