Phenotypic analysis of the medullary-type CD4 CD8+ (CD8SP) thymocytes has revealed phenotypic heterogeneity within this cell population. The phenotype of mature peripheral CD8+T cells is TCRαβ+CD3+Qa-2+HSA3G11 6C10 ...Phenotypic analysis of the medullary-type CD4 CD8+ (CD8SP) thymocytes has revealed phenotypic heterogeneity within this cell population. The phenotype of mature peripheral CD8+T cells is TCRαβ+CD3+Qa-2+HSA3G11 6C10 , whereas in the medullary-type CD8SP thymocytes, 20% are Qa-2+; 33%, HAS ; 30%, 3G11 ; and 70% are 6C10 . The disparate expression patterns of these four cell surface markers suggest that medullary-type CD8SP thymocytes may undergo phenotypic maturation process. According to the distribution of these four celi surface markers, six subgroups of CD8SP thymocytes have been identified. The precursor-progeny relationship along with developmental pathway is postulated as follows: 6C10+HSA+3G11 Qa-2 - 6C10+HSA+ 3G11+Qa-2 6C10 HSA+3G11+Qa-2 -6C10 HSA 3G11 +Qa-2 -6C10 HSA 3G11 Qa-2 -6C10 HA S 3G11 Qa-2+, the cells inthe last subgroup exit the thymus and home into periphery.展开更多
Traditional monolayer culture is still the common method used in hepatocarcinoma cell culture in vitro. For lack of the structure similar to that in vivo, it is disadvantageous in the research of the relation between ...Traditional monolayer culture is still the common method used in hepatocarcinoma cell culture in vitro. For lack of the structure similar to that in vivo, it is disadvantageous in the research of the relation between structure and function. We established a three-dimensional (3-D) culture model of human hepatocarcinoma cell (BEL-7402) in vitro by using microcarrier cytodex-3 in static condition. The results of SEM, TEM, enzyme activity and flow cytometry indicated that the cells were polygonal-shaped and arranged in multilayers. Intercellular space was 0.5-2.0 (Am wide where lots of microvilli could be seen. Adjacent cells were connected with desmosomes and localized membrane projects. More than 90% of the cells were viable and maintained the consumption of glucose and the expression of EGF receptor. The intracellular ALT, AST and LDH-L activities were higher in 3-D culture than those of monolayer culture. Compared with monolayer culture, this 3-D culture with the structure similar to trabecular展开更多
文摘Phenotypic analysis of the medullary-type CD4 CD8+ (CD8SP) thymocytes has revealed phenotypic heterogeneity within this cell population. The phenotype of mature peripheral CD8+T cells is TCRαβ+CD3+Qa-2+HSA3G11 6C10 , whereas in the medullary-type CD8SP thymocytes, 20% are Qa-2+; 33%, HAS ; 30%, 3G11 ; and 70% are 6C10 . The disparate expression patterns of these four cell surface markers suggest that medullary-type CD8SP thymocytes may undergo phenotypic maturation process. According to the distribution of these four celi surface markers, six subgroups of CD8SP thymocytes have been identified. The precursor-progeny relationship along with developmental pathway is postulated as follows: 6C10+HSA+3G11 Qa-2 - 6C10+HSA+ 3G11+Qa-2 6C10 HSA+3G11+Qa-2 -6C10 HSA 3G11 +Qa-2 -6C10 HSA 3G11 Qa-2 -6C10 HA S 3G11 Qa-2+, the cells inthe last subgroup exit the thymus and home into periphery.
基金This work was supported by the Space Cell Culture.
文摘Traditional monolayer culture is still the common method used in hepatocarcinoma cell culture in vitro. For lack of the structure similar to that in vivo, it is disadvantageous in the research of the relation between structure and function. We established a three-dimensional (3-D) culture model of human hepatocarcinoma cell (BEL-7402) in vitro by using microcarrier cytodex-3 in static condition. The results of SEM, TEM, enzyme activity and flow cytometry indicated that the cells were polygonal-shaped and arranged in multilayers. Intercellular space was 0.5-2.0 (Am wide where lots of microvilli could be seen. Adjacent cells were connected with desmosomes and localized membrane projects. More than 90% of the cells were viable and maintained the consumption of glucose and the expression of EGF receptor. The intracellular ALT, AST and LDH-L activities were higher in 3-D culture than those of monolayer culture. Compared with monolayer culture, this 3-D culture with the structure similar to trabecular
