基于SSR标记辣椒品种DNA指纹图谱的构建

基于辣椒全基因组开发的SSR标记,对青海地区主栽的26份主栽辣椒品种进行遗传多样性分析并构建分子指纹图谱。结果从117对SSR标记中筛选出6对扩增稳定,多态性较好的引物,6对标记共扩增到36个基因位点,多态性比例为97%;26份青海地区主栽辣椒品种之间遗传相似系数为0.42~1.00,在GS 0.5处可将26份辣椒材料聚为4类。优选pepperSSR01、pepperSSR04、pepperSSR06、pepperSSR08这4对引物组合便可准确快速地区分供试辣椒材料。

辣椒单株结果数性状的主基因+多基因遗传分析

为探究辣椒单株结果数的遗传机制,以单株结果数差异较大的辣椒材料XHB(P_(1))和B14-01(P_(2))为亲本,构建四世代遗传家系即P_(1)、P_(2)、F_(1)、F_(2)。运用主基因+多基因多世代联合分析法,研究辣椒单株结果数的遗传规律。结果表明:辣椒单株结果数符合2对加性-显性-上位性主基因模型(2MG-ADI)。2对主基因的加性效应值d a、d b分别为-16.33、-13.05,2对主基因的显性效应值h a、h b分别为-10.02、-2.51。2对主基因间的加性×显性(j_(ab))互作效应和显性×加性(j ba)互作效应的效应值分别为8.69和12.93,加性×加性上位性(i)互作效应值为6.86,显性×显性(l)的互作效应值为7.23,主基因间的效应以加性效应为主,其次是加性×显性上位性互作效应。主基因遗传率为68.10%,环境引起的变异占比31.9%,表明环境对辣椒结果数的影响相对较小。相关研究结果为不同单株结果数辣椒新品种的选育及相关基因的定位等研究奠定了基础。

青海高原地区辣椒嫁接砧木品种评价与筛选

为筛选出适宜青海高原地区嫁接的辣椒砧木品种,本研究以青海省主栽乐都长辣椒为接穗和自根对照品种,采用劈接法对引进的11份辣椒砧木品种进行评价筛选。研究表明:格拉芙特、卡特188、东洋强势与乐都长辣椒嫁接后亲和性好、生长势强、产量显著高于对照,品质性状与对照相比无显著差异。因此,以上3个品种可作为乐都长辣椒的嫁接砧木在青海省辣椒主栽区推广种植。

Fuzzy modeling of multirate sampled nonlinear systems based on multi-model method

Based on the multi-model principle, the fuzzy identification for nonlinear systems with multirate sampled data is studied.Firstly, the nonlinear system with multirate sampled data can be shown as the nonlinear weighted combination of some linear models at multiple local working points. On this basis, the fuzzy model of the multirate sampled nonlinear system is built. The premise structure of the fuzzy model is confirmed by using fuzzy competitive learning, and the conclusion parameters of the fuzzy model are estimated by the random gradient descent algorithm. The convergence of the proposed identification algorithm is given by using the martingale theorem and lemmas. The fuzzy model of the PH neutralization process of acid-base titration for hair quality detection is constructed to demonstrate the effectiveness of the proposed method.