Fatty acid-bindi ng proteins(FABPs)are a family of lipid chaper on es,which con tribute to systemic metabolic regulati on through diverse lipid signalings.In this study,a midgut-specific FABP gene(Slfabp2)was cloned f...Fatty acid-bindi ng proteins(FABPs)are a family of lipid chaper on es,which con tribute to systemic metabolic regulati on through diverse lipid signalings.In this study,a midgut-specific FABP gene(Slfabp2)was cloned from Spodoptera litura.RT-PCR and Western blot analysis indicated that RNA and protein levels of S/FABP2 gradually increased and reached a peak at the prepupal stage and maintained a high level during the pupal stage.The expression of S/FABP2 protein was induced by starvation treatment.In vitro binding assay revealed that the recombinant S/FABP2 had high mffinities of binding Iong-chain fatty acids,such as palmitic acid,arachidonate and oleic acid.The results suggest that S/FABP2 may have a unique function that transports intracellular fatty acids and can regulate the metabolism of lipids in metamorphosis.This work provides experimental clues for understanding the potential function of S/FABP2 in fatty acid metabolism in S.litura.展开更多
Annual loss of crop yields due to agricultura I in sect pests are approximately 10%.Effective and safe management of insect pests would reduce the loss of crop production.In sects live in an environment where they nee...Annual loss of crop yields due to agricultura I in sect pests are approximately 10%.Effective and safe management of insect pests would reduce the loss of crop production.In sects live in an environment where they need to deal with biological and non-biological factors that impact their physiological and developmental activities to survive and expand their population.These environmental factors in elude,but not limited to,phytochemicals in the host plants they feed on,toxic compounds,such as insecticides sprayed by human,parasitoid,microbes,temperature and drought stress.In the Ion g-term evolution,in sects have developed sophisticated strategies to adapt the harmful factors against them.For example,to feed on different host plants,in sects develop effective and comprehensive olfactory and gustatory receptor systems and detoxification enzyme systems to deal with the secondary toxic phytochemicals.These olfactory and gustatory receptor and detoxification enzyme systems contain multiple superfamilies of proteins and enzymes,such as cytochrome P450s,glutathione S-transferases(GSTs)and esterases,together to form multiple preventive and protection barriers along with the regulation and function of the endocrine systems,which synthesize and secrete hormones and neuropeptides circulating to the different target tissues and organs to guarantee the normal growth and development.On the other hand,in sects also adjust their feeding behaviors and metabolism pathways,as well as even the nutrient components in the host plants by cha nging the expression patter ns of related genes to promote the nutrie nt intake and utilization.In sects and their host plants ultimately establish a cooperative and antagonistic relationship during evolution.展开更多
基金The study was supported by the National Natural Science Foundation of China(31330071 and 31772519)and the Natural Science Foundation of Guangdong Province,China(2017A030313210).
文摘Fatty acid-bindi ng proteins(FABPs)are a family of lipid chaper on es,which con tribute to systemic metabolic regulati on through diverse lipid signalings.In this study,a midgut-specific FABP gene(Slfabp2)was cloned from Spodoptera litura.RT-PCR and Western blot analysis indicated that RNA and protein levels of S/FABP2 gradually increased and reached a peak at the prepupal stage and maintained a high level during the pupal stage.The expression of S/FABP2 protein was induced by starvation treatment.In vitro binding assay revealed that the recombinant S/FABP2 had high mffinities of binding Iong-chain fatty acids,such as palmitic acid,arachidonate and oleic acid.The results suggest that S/FABP2 may have a unique function that transports intracellular fatty acids and can regulate the metabolism of lipids in metamorphosis.This work provides experimental clues for understanding the potential function of S/FABP2 in fatty acid metabolism in S.litura.
文摘Annual loss of crop yields due to agricultura I in sect pests are approximately 10%.Effective and safe management of insect pests would reduce the loss of crop production.In sects live in an environment where they need to deal with biological and non-biological factors that impact their physiological and developmental activities to survive and expand their population.These environmental factors in elude,but not limited to,phytochemicals in the host plants they feed on,toxic compounds,such as insecticides sprayed by human,parasitoid,microbes,temperature and drought stress.In the Ion g-term evolution,in sects have developed sophisticated strategies to adapt the harmful factors against them.For example,to feed on different host plants,in sects develop effective and comprehensive olfactory and gustatory receptor systems and detoxification enzyme systems to deal with the secondary toxic phytochemicals.These olfactory and gustatory receptor and detoxification enzyme systems contain multiple superfamilies of proteins and enzymes,such as cytochrome P450s,glutathione S-transferases(GSTs)and esterases,together to form multiple preventive and protection barriers along with the regulation and function of the endocrine systems,which synthesize and secrete hormones and neuropeptides circulating to the different target tissues and organs to guarantee the normal growth and development.On the other hand,in sects also adjust their feeding behaviors and metabolism pathways,as well as even the nutrient components in the host plants by cha nging the expression patter ns of related genes to promote the nutrie nt intake and utilization.In sects and their host plants ultimately establish a cooperative and antagonistic relationship during evolution.
