γ谷氨酰胺转移酶与Framingham危险评分的相关性分析

【目的】γ谷氨酰胺转移酶(GGT)近年来被发现与动脉粥样硬化及冠心病相关,本研究旨在探讨血清GGT水平与十年心脏病危险评分——Framingham危险评分(FRS)之间的相关性,以期能更早期发现心血管疾病的中高危患者。【方法】本研究入组了9 544名40岁及以上的广州社区居民,均来自于一项基于广州社区居民的横断面流行病学研究。GGT根据四分位数分组:Q1<15 U/L(n=2 407例)、Q2 15～19.9 U/L(n=2 302例)、Q3 20～28.9 U/L(n=2 442例)、Q4≥29 U/L(n=2 393例)。GGT与十年心血管疾病中高危风险(FRS≥10%)进行Logistic多重回归分析。【结果】相对于GGT最低四分位数组,随着GGT水平升高,FRS≥10%的比例增高(P<0.01),这种相关关系在校正了混杂因素后仍然存在,最高四分位数组的风险是最低四分位数组的1.72倍(95%置信区间1.28-2.29)。【结论】γ谷氨酰胺转移酶与十年心脏病危险评分FRS存在相关关系,建议常规体检早期重视GGT,有利于早期评估及干预心血管疾病中高危人群。

甲状腺功能正常人群甲状腺过氧化物酶抗体和甲状腺体积的关系

目的:研究碘摄入足量、甲状腺功能正常人群甲状腺过氧化物酶抗体(TPOAb)和甲状腺体积的关系。方法:对广州市两个社区2682名常住成年居民进行调查,包括体格检查、检测血清促甲状腺素(TSH)、TPOAb和尿碘,甲状腺超声检查计算甲状腺体积。结果:排除既往甲状腺疾病及甲状腺功能异常者,共2422人(男/女:1054/1368,中位年龄45.89岁)纳入研究,TSH水平1.76 mIU/L,尿碘中位数为128μg/L。TPOAb阳性率为10.33%,随着年龄的增大,TPOAb阳性率逐渐增高,甲状腺体积也逐渐增大。简单线性回归分析以及多重线性回归分析均显示本组人群甲状腺体积和TPOAb滴度呈正相关。按不同的TPOAb滴度把本组研究人群划分为五组,同样显示随着TPOAb滴度等级的升高,甲状腺体积逐渐增大,在女性中尤为突出。结论:在碘摄入足量、甲状腺功能正常人群中TPOAb滴度与甲状腺体积呈正相关关系。

基于Unity3d的虚拟实验设计与开发

虚拟实验是虚拟现实技术在教育领域的应用,将虚拟现实技术与实验教学相结合,基于Unity3d的虚拟实验开发的一般流程,设计并制作《喷油泵拆装》虚拟实验,教学实践应用表明,虚拟实验具有良好的三维交互性、可操作性和场景逼真等特点,能够激发学生的创造力和想象力,在实验教学领域具有较大的应用价值。

高氯酸盐的检测及去除技术研究进展

综述了高氯酸盐的来源、危害及检测、去除技术的研究进展,并对未来研究方向、发展趋势进行了展望。高氯酸盐是一种重要的无机污染物,被广泛应用在烟火制造、军火工业和航天工业中,作为强氧化剂。高氯酸盐可通过空气、食物、饮用水等途径暴露于人体,在生物体内会导致人体和哺乳动物的甲状腺功能异常而表现出毒性,影响生物体的正常生理功能。随着其在水体、土壤、食物中频繁检出,引起了研究者的重视。目前对高氯酸盐的研究仍缺乏全面深入的调查数据,以期为深入探索高氯酸盐污染问题提供一些有用的参考。

Influences of the interferon induced transmembrane protein I on the proliferation, invasion, and metastasis of the colorectal cancer SW480 cell lines

Background Interferon-induced transmembrane protein 1 （IFITM1） has been identified as a molecular marker of the colorectal tumors; however its influences on the biological behaviors of the colorectal cancer cells are currently unknown.We aimed to study the influences of IFITM1 on the proliferation,invasion,and metastasis of the colorectal cancer SW480 cell lines.Methods We constructed IFITM1/pEGFP-C3 recombinant plasmids and transfected them into the colorectal cancer SW480 cell lines.IFITM1/pEGFP-C3 recombinant plasmids were identified by means of immunofluorescence,laser confocal scanning microscopy,and reverse transcription polymerase chain reaction.IFITM1/SW480 cells with stable over-expression of IFITM1 were confirmed by G418 screening.The influences of IFITM1 on the proliferation of the SW480 cell lines were investigated by MTT assay and tumor transplantation experiments in nude mice.Cell invasion experiments were performed to determine the invasion capacity of the IFITM1/SW480 cells.Matrix metalloproteinase 2 （MMP-2） and MMP-9 activities were detected by the gelatin zymographic analysis,and MMP-9 expression by the Western blotting analysis.Results IFITM1/pEGFP-C3 recombinant plasmids were successfully constructed in this study,and the IFITM1/SW480 cells with stable IFITM1 gene over-expression were confirmed by G418 screening.MTT results showed that the proliferation of the IFITM1/SW480 cells was significantly enhanced （P 〈0.01）.Tumors were harvested from four weeks old mice.Tumor volumes were （1347.00±60.94） mm3,（1032.40±111.38） mm3 and （1018.78±28.83） mm3; and tumor weights were （1522.34±62.76） mg,（1137.78±97.22） mg and （1155.76±133.31） mg for mice inoculated with the IFITM1/SW480 cells,pEGFP-C3/SW480 cells and SW480 cells,respectively.Tumor volumes and weights from mice inoculated with the IFITM1/SW480 cells were significantly increased （P 〈0.01）.In addition,the numbers of the SW480 cells and IFITM1/SW480 cells that migrated through Matrigel were 448.64±38.09 and 540.45±44.61,respectively; so the invasive ability of the SW480 cells transfected with IFITM1 gene was significantly greater than that of the SW480 cells （P 〈0.01）.Gelatin zymographic analysis showed that MMP-9 and MMP-2 protein activities in the IFITM1/SW480 cells were significantly enhanced,and Western blotting analysis showed that MMP-9 expression in the IFITM1/SW480 cells was also increased.Conclusion IFITMl can enhance the proliferation,invasion,and metastasis of the colorectal cancer SW480 cell lineS.

绘画疗法治疗自我意识低水平矮小症患儿的应用研究

目的探讨绘画疗法治疗自我意识低水平矮小症患儿的临床效果。方法采用随机数字法将60例自我意识低水平矮小症患儿随机分为两组(n=30),对照组行纯生长激素治疗,观察组在对照组的基础上联合绘画疗法。干预6个月后,比较两组患儿身高、情绪状态、自我意识以及对药物治疗的依从性、服药后副反应情况。结果干预3个月后,观察组患儿身高增长速度高于对照组,两组间差异不存在统计学意义(P>0.05);干预6个月后,两组患儿身高增长速度均明显高于治疗3个月时,且观察组患儿明显高于对照组,两组间差异具有统计学意义(P<0.05)。干预后,观察组患儿骨龄对应的身高标准差分值明显少于对照组、预测成年身高明显高于对照组,两组间差异具有统计学意义(P<0.05)。干预后,观察组对生长激素治疗依从性明显高于对照组(93.33%vs 76.67%),两组间差异具有统计学意义(P<0.05)。干预后,观察组患儿躯体外貌与属性、合群、幸福与满足评分明显高于对照组,焦虑评分明显低于对照组,两组间差异具有统计学意义(P<0.05)。观察组患儿SDS、SAS评分明显低于对照组,两组间差异具有统计学意义(P<0.05)。干预后,观察组患儿眼睑肿胀、关节疼痛、四肢水肿等药物副反应总发生率明显低于对照组(6.67%vs 23.33%),两组间差异具有统计学意义(P<0.05)。结论在生长激素治疗的基础上联合绘画疗法可有效提高自我意识低水平矮小症患儿的治疗依从性,降低药物副反应,改善患儿情绪状态及自我意识,有利于促进患儿身高增长速度的提升。