A surface-enhanced Raman scattering(SERS) optical fiber sensor was prepared by the laser-induced deposition ofAg nanoparticle membrane on a silica optical fiber tip, which was applied to the real time SERS spectral ...A surface-enhanced Raman scattering(SERS) optical fiber sensor was prepared by the laser-induced deposition ofAg nanoparticle membrane on a silica optical fiber tip, which was applied to the real time SERS spectral monitoring on the biorecognition of biotin/avidin. The bioidentification of biotin/avidin was carried out through a indirect method, in which the bioidentification is based on the SERS response signal of a labeled dye(Atto610) after its fluorescence has been quenched totally by the deposited Ag nanoparticle membrane. By SERS monitoring the bioidentification process of biotin/avidin, it has been found that this recognition process is finished in 40 min. The lowest detection concentration of biotin is 1.0 × 10^-7 mg/mL. This research is promising in the application of immunoassays on line and in vivo.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.91027010, 21073073, 20903043, 20973075), the Research Fund for the Doctoral Program of Higher Education of China(No.20090061120089) and the National Instrumentation Program(NIP) of the Ministry of Science and Technology of China(No.2011YQ03012408).
文摘A surface-enhanced Raman scattering(SERS) optical fiber sensor was prepared by the laser-induced deposition ofAg nanoparticle membrane on a silica optical fiber tip, which was applied to the real time SERS spectral monitoring on the biorecognition of biotin/avidin. The bioidentification of biotin/avidin was carried out through a indirect method, in which the bioidentification is based on the SERS response signal of a labeled dye(Atto610) after its fluorescence has been quenched totally by the deposited Ag nanoparticle membrane. By SERS monitoring the bioidentification process of biotin/avidin, it has been found that this recognition process is finished in 40 min. The lowest detection concentration of biotin is 1.0 × 10^-7 mg/mL. This research is promising in the application of immunoassays on line and in vivo.