本研究以温州芜菁(Brassica rapa L. em. Matzg.,2n=20,AA)为母本,水果紫苤蓝(B. oleracea L. var. caulorapa DC,2n=18,CC)为父本进行远缘杂交,创造种间杂种。通过花期剥蕾授粉、子房培养共获得2株杂种,经扩繁得到无性苗19株,经形态学...本研究以温州芜菁(Brassica rapa L. em. Matzg.,2n=20,AA)为母本,水果紫苤蓝(B. oleracea L. var. caulorapa DC,2n=18,CC)为父本进行远缘杂交,创造种间杂种。通过花期剥蕾授粉、子房培养共获得2株杂种,经扩繁得到无性苗19株,经形态学分析、细胞学观察和RAPD分子鉴定出2株均为温州芜菁×紫苤蓝的真杂种,后利用染色体加倍获得异源四倍体新型芜菁甘蓝新种质材料(AACC,2n=38)。两杂种株系出现茎色分离,根部与茎部均有膨大,但程度低于亲本。异源四倍体的花器官较大,花药发育正常,可育花粉率高。本研究所创制的异源四倍体新种质,在一定程度上拓展了芸薹属蔬菜种质资源的遗传基础。展开更多
[ Objective] The research aimed to study the low-cost medium instead of MRS to produce lactobacillus suspension and to provide a theoretical basis for the large-scale production of stalk fermented by lactobacillus. [ ...[ Objective] The research aimed to study the low-cost medium instead of MRS to produce lactobacillus suspension and to provide a theoretical basis for the large-scale production of stalk fermented by lactobacillus. [ Method] Different concentrations of glucose and sodium chloride were added into the cooking liquor of maize flour, and lactobacillus was inoculated to ferment the stalk. The changes of pH were measured and compared with MRS medium. The optimum low-cost medium for producing lactobacillus and fermenting stalk was selected to ferment stalk. And the feeding experiment of cattle was made with fermented stalk and unfermented stalk to study the consumption quantity of different kinds of feeds. [ Resuit] The changes of pH in cornmeal and MRS media had the same tendency, but the decrease of pH in cornmeal medium was faster. The consumed quantity of fermented stalk feed was larger than that of unfermented feeds. [ Conclusion] Cornmeal medium can be used instead of MRS to produce lactobacillus and the production cost decreased more than 80%.展开更多
文摘本研究以温州芜菁(Brassica rapa L. em. Matzg.,2n=20,AA)为母本,水果紫苤蓝(B. oleracea L. var. caulorapa DC,2n=18,CC)为父本进行远缘杂交,创造种间杂种。通过花期剥蕾授粉、子房培养共获得2株杂种,经扩繁得到无性苗19株,经形态学分析、细胞学观察和RAPD分子鉴定出2株均为温州芜菁×紫苤蓝的真杂种,后利用染色体加倍获得异源四倍体新型芜菁甘蓝新种质材料(AACC,2n=38)。两杂种株系出现茎色分离,根部与茎部均有膨大,但程度低于亲本。异源四倍体的花器官较大,花药发育正常,可育花粉率高。本研究所创制的异源四倍体新种质,在一定程度上拓展了芸薹属蔬菜种质资源的遗传基础。
基金funded by the National Special Research Fund for Public Welfare (Agriculture) of China (200803033B0502)
文摘[ Objective] The research aimed to study the low-cost medium instead of MRS to produce lactobacillus suspension and to provide a theoretical basis for the large-scale production of stalk fermented by lactobacillus. [ Method] Different concentrations of glucose and sodium chloride were added into the cooking liquor of maize flour, and lactobacillus was inoculated to ferment the stalk. The changes of pH were measured and compared with MRS medium. The optimum low-cost medium for producing lactobacillus and fermenting stalk was selected to ferment stalk. And the feeding experiment of cattle was made with fermented stalk and unfermented stalk to study the consumption quantity of different kinds of feeds. [ Resuit] The changes of pH in cornmeal and MRS media had the same tendency, but the decrease of pH in cornmeal medium was faster. The consumed quantity of fermented stalk feed was larger than that of unfermented feeds. [ Conclusion] Cornmeal medium can be used instead of MRS to produce lactobacillus and the production cost decreased more than 80%.
