期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
偶联CD206抗体载Fe3O4的PLGA纳米微球促进巨噬细胞M1型极化 被引量:4
1
作者 付倩梅 唐华明 +3 位作者 张鹏 阙克婷 刘作金 周赟 《南方医科大学学报》 CAS CSCD 北大核心 2020年第2期246-254,共9页
目的通过构建纳米微球靶向性地提高巨噬细胞中的铁浓度来增强抗肿瘤免疫。方法采用W/O/W复乳化溶剂扩散法制备CD206单克隆抗体表面修饰的载Fe3O4聚乳酸羟基乙酸(PLGA)纳米微球。使用马尔文粒径检测仪测定微粒直径,Zeta电位法测定Zeta电... 目的通过构建纳米微球靶向性地提高巨噬细胞中的铁浓度来增强抗肿瘤免疫。方法采用W/O/W复乳化溶剂扩散法制备CD206单克隆抗体表面修饰的载Fe3O4聚乳酸羟基乙酸(PLGA)纳米微球。使用马尔文粒径检测仪测定微粒直径,Zeta电位法测定Zeta电位。用铁测定试剂盒测定Fe3O4的包封率。采用免疫荧光实验检测CD206抗体与巨噬细胞的结合及靶向性。Western blot、qRT-PCR检测巨噬细胞的极化指数。并用BALB/C-57小鼠皮下肿瘤模型验证纳米微球促进肿瘤相关巨噬细胞(TAM)的极化状态。结果纳米微球的平均直径在260~95 nm,Zeta电位值在-19^-33 MV。Fe3O4的包封率在65%~75%。流式细胞术检测CD206单克隆抗体与PLGA微球偶联率为65%~70%,免疫荧光实验证实了PLGA微球与CD206高表达巨噬细胞的靶向结合能力。Western blot和qRT-PCR证实了偶联CD206抗体载Fe3O4的PLGA纳米微球(CD206-Fe3O4-PLGA)和载Fe3O4的PLGA纳米微球(Fe3O4-PLGA)促进TNF-α、iNOS和IL-1β的表达(P<0.05)。小鼠肿瘤模型研究证实CD206-Fe3O4-PLGA纳米颗粒促进TAMs中CD86的表达(P<0.05)。结论PLGA纳米微球具有均匀的粒子的大小及Zeta电位,以及较好的抗体偶联效率及纳米铁包封率,同时偶联CD206的PLGA微球能够较好的靶向结M2型巨噬细胞,并通过释放包被的Fe3O4促进巨噬细胞的M1型极化。本研究为肿瘤的免疫治疗提供了一种潜在的方法。 展开更多
关键词 PLGA微球 纳米氧化铁 靶向药物转运 肿瘤的免疫治疗
下载PDF
Heat Transfer Characteristics of Printed Circuit Heat Exchanger with Supercritical Carbon Dioxide and Molten Salt 被引量:4
2
作者 LAO Jiewei fu qianmei +2 位作者 WANG Weilong DING Jing LU Jianfeng 《Journal of Thermal Science》 SCIE EI CAS CSCD 2021年第3期880-891,共12页
Molten salt and supercritical carbon dioxide(S-CO_(2))are important high temperature heat transfer media,but molten salt/S-CO_(2) heat exchanger has been seldom reported.In present paper,heat transfer in printed circu... Molten salt and supercritical carbon dioxide(S-CO_(2))are important high temperature heat transfer media,but molten salt/S-CO_(2) heat exchanger has been seldom reported.In present paper,heat transfer in printed circuit heat exchanger(PCHE)with molten salt and S-CO_(2) is simulated and analyzed.Since S-CO_(2) can be drove along passage wall by strong buoyancy force with large density difference,its heat transfer is enhanced by natural convection.In inlet region,natural convection weakens along flow direction with decreasing Richardson number,and the thermal boundary layer becomes thicker,so local heat transfer coefficient of S-CO_(2) significantly decreases.In outlet region,turbulent kinetic energy gradually increases,and then heat transfer coefficient increases for turbulent heat transfer enhancement.Compared with transcritical CO_(2) with lower inlet temperature,local heat transfer coefficient of S-CO_(2) near inlet is lower for smaller Richardson number,while it will be higher for larger turbulent kinetic energy near outlet.Performance of PCHE is mainly determined by the pressure drop in molten salt passage and the heat transfer resistance in S-CO_(2) passage.When molten salt passage width increases,molten salt pressure drop significantly decreases,and overall heat transfer coefficient slightly changes,so the comprehensive performance of PCHE is improved.As a result,PCHE unit with three semicircular passages and one semi-elliptic passage has better performance. 展开更多
关键词 heat exchanger molten salt supercritical carbon dioxide numerical simulation
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部