目的：分析酒制对女贞子饮片中主要化学成分含量的影响,为完善女贞子及酒女贞子饮片的质量标准提供实验依据。方法：采用HPLC,流动相乙腈-水梯度洗脱,流速1.0 m L·min-（-1）,检测波长224 nm,柱温35℃,对女贞子及酒女贞子饮片中主...目的：分析酒制对女贞子饮片中主要化学成分含量的影响,为完善女贞子及酒女贞子饮片的质量标准提供实验依据。方法：采用HPLC,流动相乙腈-水梯度洗脱,流速1.0 m L·min-（-1）,检测波长224 nm,柱温35℃,对女贞子及酒女贞子饮片中主要化学成分进行定量分析。结果：与相同企业或同一企业同一批次女贞子饮片比较,酒女贞子饮片中特女贞苷和橄榄苦苷的含量均明显降低,红景天苷、酪醇和羟基酪醇的含量有不同程度的升高;其中红景天苷、酪醇及羟基酪醇的质量分数分别升高了200%,5%,40%以上。结论：酒制后女贞子饮片化学成分含量的变化主要是由于特女贞苷在高温及水蒸气的作用下会水解生成红景天苷,红景天苷进一步水解生成酪醇;橄榄苦苷在高温下不稳定,同样发生水解,生成羟基酪醇;从而导致特女贞苷和橄榄苦苷的含量显著降低,红景天苷、酪醇和羟基酪醇的含量增高。展开更多
Objective:To observe the effect of amygdalin on liver fibrosis in a liver fibrosis mouse model,and the underlying mechanisms were partly dissected in vivo and in vitro.Methods:Thirty-two male mice were randomly divide...Objective:To observe the effect of amygdalin on liver fibrosis in a liver fibrosis mouse model,and the underlying mechanisms were partly dissected in vivo and in vitro.Methods:Thirty-two male mice were randomly divided into 4 groups,including control,model,low-and high-dose amygdalin-treated groups,8 mice in each group.Except the control group,mice in the other groups were injected intraperitoneally with 10%carbon tetrachloride(CCl4)-olive oil solution 3 times a week for 6 weeks to induce liver fibrosis.At the first 3 weeks,amygdalin(1.35 and 2.7 mg/kg body weight)were administered by gavage once a day.Mice in the control group received equal quantities of subcutaneous olive oil and intragastric water from the fourth week.At the end of 6 weeks,liver tissue samples were harvested to detect the content of hydroxyproline(Hyp).Hematoxylin and eosin and Sirius red staining were used to observe the inflammation and fibrosis of liver tissue.The expressions of collagenⅠ(Col-Ⅰ),alpha-smooth muscle actin(α-SMA),CD31 and transforming growth factorβ(TGF-β)/Smad signaling pathway were detected by immunohistochemistry,quantitative real-time polymerase chain reaction and Western blot,respectively.The activation models of hepatic stellate cells,JS-1and LX-2 cells induced by TGF-β1 were used in vitro with or without different concentrations of amygdalin(0.1,1,10μmol/L).The effect of different concentrations of amygdalin on the expressions of liver sinusoidal endothelial cells(LSECs)dedifferentiation markers CD31 and CD44 were observed.Results:High-dose of amygdalin significantly reduced the Hyp content and percentage of collagen positive area,and decreased the mRNA and protein expressions of Col-Ι,α-SMA,CD31 and p-Smad2/3 in liver tissues of mice compared to the model group(P<0.01).Amygdalin down-regulated the expressions of Col-Ⅰandα-SMA in JS-1 and LX-2 cells,and TGFβR1,TGFβR2 and p-Smad2/3 in LX-2 cells compared to the model group(P<0.05 or P<0.01).Moreover,1 and 10μmol/L amygdalin inhibited the mRNA and protein expressions of CD31 in LSECs and increased CD44 expression compared to the model group(P<0.05 or P<0.01).Conclusions:Amygdalin can dramatically alleviate liver fibrosis induced by CCl4 in mice and inhibit TGF-β/Smad signaling pathway,consequently suppressing HSCs activation and LSECs dedifferentiation to improve angiogenesis.展开更多
文摘目的：分析酒制对女贞子饮片中主要化学成分含量的影响,为完善女贞子及酒女贞子饮片的质量标准提供实验依据。方法：采用HPLC,流动相乙腈-水梯度洗脱,流速1.0 m L·min-（-1）,检测波长224 nm,柱温35℃,对女贞子及酒女贞子饮片中主要化学成分进行定量分析。结果：与相同企业或同一企业同一批次女贞子饮片比较,酒女贞子饮片中特女贞苷和橄榄苦苷的含量均明显降低,红景天苷、酪醇和羟基酪醇的含量有不同程度的升高;其中红景天苷、酪醇及羟基酪醇的质量分数分别升高了200%,5%,40%以上。结论：酒制后女贞子饮片化学成分含量的变化主要是由于特女贞苷在高温及水蒸气的作用下会水解生成红景天苷,红景天苷进一步水解生成酪醇;橄榄苦苷在高温下不稳定,同样发生水解,生成羟基酪醇;从而导致特女贞苷和橄榄苦苷的含量显著降低,红景天苷、酪醇和羟基酪醇的含量增高。
基金Supported by National Natural Science Foundation of China(Nos.81530101,81973613 and 81603681)Shanghai Rising-Star Program(No.19QA1408900)。
文摘Objective:To observe the effect of amygdalin on liver fibrosis in a liver fibrosis mouse model,and the underlying mechanisms were partly dissected in vivo and in vitro.Methods:Thirty-two male mice were randomly divided into 4 groups,including control,model,low-and high-dose amygdalin-treated groups,8 mice in each group.Except the control group,mice in the other groups were injected intraperitoneally with 10%carbon tetrachloride(CCl4)-olive oil solution 3 times a week for 6 weeks to induce liver fibrosis.At the first 3 weeks,amygdalin(1.35 and 2.7 mg/kg body weight)were administered by gavage once a day.Mice in the control group received equal quantities of subcutaneous olive oil and intragastric water from the fourth week.At the end of 6 weeks,liver tissue samples were harvested to detect the content of hydroxyproline(Hyp).Hematoxylin and eosin and Sirius red staining were used to observe the inflammation and fibrosis of liver tissue.The expressions of collagenⅠ(Col-Ⅰ),alpha-smooth muscle actin(α-SMA),CD31 and transforming growth factorβ(TGF-β)/Smad signaling pathway were detected by immunohistochemistry,quantitative real-time polymerase chain reaction and Western blot,respectively.The activation models of hepatic stellate cells,JS-1and LX-2 cells induced by TGF-β1 were used in vitro with or without different concentrations of amygdalin(0.1,1,10μmol/L).The effect of different concentrations of amygdalin on the expressions of liver sinusoidal endothelial cells(LSECs)dedifferentiation markers CD31 and CD44 were observed.Results:High-dose of amygdalin significantly reduced the Hyp content and percentage of collagen positive area,and decreased the mRNA and protein expressions of Col-Ι,α-SMA,CD31 and p-Smad2/3 in liver tissues of mice compared to the model group(P<0.01).Amygdalin down-regulated the expressions of Col-Ⅰandα-SMA in JS-1 and LX-2 cells,and TGFβR1,TGFβR2 and p-Smad2/3 in LX-2 cells compared to the model group(P<0.05 or P<0.01).Moreover,1 and 10μmol/L amygdalin inhibited the mRNA and protein expressions of CD31 in LSECs and increased CD44 expression compared to the model group(P<0.05 or P<0.01).Conclusions:Amygdalin can dramatically alleviate liver fibrosis induced by CCl4 in mice and inhibit TGF-β/Smad signaling pathway,consequently suppressing HSCs activation and LSECs dedifferentiation to improve angiogenesis.
