Smut is a serious disease in Job's tears,also known as adlay,and contributes to the reduction of crop yield in agricultural fields.In this study,the key pathogenic fungi in adlay smut disease were first identified...Smut is a serious disease in Job's tears,also known as adlay,and contributes to the reduction of crop yield in agricultural fields.In this study,the key pathogenic fungi in adlay smut disease were first identified by internal transcribed spacer region(ITS)high-throughput sequencing and then used to elucidate the composition and diversity of the fungal community in adlay smut.Results indicate that an abundance of operational taxonomic units(OTUs)were detected in the infected involucres of flowering plants and the OTUs were classified to nine phyla,20 classes,45 orders,90 families and 119 genera.A total of 4986 OTUs clustered together,sharing six core OTUs in all samples,while 145 OTUs were shared among four geographical groups.The Shannon and Simpson indices ranged from 0.137–1.629 and 0.357–0.970,respectively.Community diversity ranked as Anshun(AS)>Qinglong(QL)>Xingren(XR)>Xingyi(XY)among the four geographical groups by Shannon and Simpson indices,exhibiting complex community diversities among accessions and geographical groups.The richness and diversity data imply a weak relationship between the accession community richness and geographical origins of samples.Two closely related fungal genera,Sporisorium and Ustilago,were implicated as causes of smut disease.The genus Sporisorium appears to be more commonly found among accessions and thus is more likely to be the fungal pathogen causing smut in adlay.This work can facilitate strategies to control and prevent smut infection to improve adlay yield.展开更多
目的:研究贵州喀斯特地区铁皮石斛Dendrobium officinale Kimura et Migo仿野生种植关键技术。方法:在兴义市则戎镇干嘎村干嘎丫口、长冲村小东山、冷洞村烘纳组和南盘江镇田房村坡墰组等地,开展铁皮石斛仿野生种植试验,研究仿野生种植...目的:研究贵州喀斯特地区铁皮石斛Dendrobium officinale Kimura et Migo仿野生种植关键技术。方法:在兴义市则戎镇干嘎村干嘎丫口、长冲村小东山、冷洞村烘纳组和南盘江镇田房村坡墰组等地,开展铁皮石斛仿野生种植试验,研究仿野生种植环境条件、种植方法、管理技术措施。结果:(1)地势开阔、光照充足、通风良好的地方种植效果好,深山峡谷、地势低洼、光照时间短、通风不良的地方种植效果差;(2)山中上部仿野生种植效果优于山中下部;(3)郁闭度以30%~50%为最好,不能大于80%;(4)经大棚驯化1~2年的种苗,种植成活率、分蘖率高,能获得适当的产量。结论:环境和种苗是影响铁皮石斛仿野生种植的关键。展开更多
基金This work was financially supported by the Science and Technology Program in Guizhou Province,China(20162608,20162569)the High-Level Innovative Talents Training Project in Guizhou Province,China(20154016)the Southwest Guizhou of Science and Technology Program,China(2017-1-2).
文摘Smut is a serious disease in Job's tears,also known as adlay,and contributes to the reduction of crop yield in agricultural fields.In this study,the key pathogenic fungi in adlay smut disease were first identified by internal transcribed spacer region(ITS)high-throughput sequencing and then used to elucidate the composition and diversity of the fungal community in adlay smut.Results indicate that an abundance of operational taxonomic units(OTUs)were detected in the infected involucres of flowering plants and the OTUs were classified to nine phyla,20 classes,45 orders,90 families and 119 genera.A total of 4986 OTUs clustered together,sharing six core OTUs in all samples,while 145 OTUs were shared among four geographical groups.The Shannon and Simpson indices ranged from 0.137–1.629 and 0.357–0.970,respectively.Community diversity ranked as Anshun(AS)>Qinglong(QL)>Xingren(XR)>Xingyi(XY)among the four geographical groups by Shannon and Simpson indices,exhibiting complex community diversities among accessions and geographical groups.The richness and diversity data imply a weak relationship between the accession community richness and geographical origins of samples.Two closely related fungal genera,Sporisorium and Ustilago,were implicated as causes of smut disease.The genus Sporisorium appears to be more commonly found among accessions and thus is more likely to be the fungal pathogen causing smut in adlay.This work can facilitate strategies to control and prevent smut infection to improve adlay yield.
文摘目的:研究贵州喀斯特地区铁皮石斛Dendrobium officinale Kimura et Migo仿野生种植关键技术。方法:在兴义市则戎镇干嘎村干嘎丫口、长冲村小东山、冷洞村烘纳组和南盘江镇田房村坡墰组等地,开展铁皮石斛仿野生种植试验,研究仿野生种植环境条件、种植方法、管理技术措施。结果:(1)地势开阔、光照充足、通风良好的地方种植效果好,深山峡谷、地势低洼、光照时间短、通风不良的地方种植效果差;(2)山中上部仿野生种植效果优于山中下部;(3)郁闭度以30%~50%为最好,不能大于80%;(4)经大棚驯化1~2年的种苗,种植成活率、分蘖率高,能获得适当的产量。结论:环境和种苗是影响铁皮石斛仿野生种植的关键。
