目的研究褐藻多酚对晚期糖基化终产物(advanced glycation end products,AGEs)生成的抑制作用及其机制。方法建立牛血清蛋白与葡萄糖体外模拟体系及食品热加工鱼肉肠模型,以不同浓度褐藻多酚为抑制剂,研究其对AGEs生成的抑制作用;通过...目的研究褐藻多酚对晚期糖基化终产物(advanced glycation end products,AGEs)生成的抑制作用及其机制。方法建立牛血清蛋白与葡萄糖体外模拟体系及食品热加工鱼肉肠模型,以不同浓度褐藻多酚为抑制剂,研究其对AGEs生成的抑制作用;通过分析模拟体系中赖氨酸和葡萄糖含量、蛋白质巯基水平及葡萄糖与牛血清蛋白的交联作用,阐明褐藻多酚对AGEs生成抑制作用机制。结果褐藻多酚对荧光性AGEs具有显著的抑制作用,100μg/g褐藻多酚反应36 h,对模拟体系AGEs生成抑制率达30.33%,50μg/mL褐藻多酚对鱼肉肠AGEs生成抑制率达41.91%。褐藻多酚处理后,模拟体系中赖氨酸和葡萄糖含量、蛋白质巯基水平显著高于对照组(P<0.05),而葡萄糖与牛血清蛋白交联条带量显著低于对照组(P<0.05)。结论褐藻多酚可能通过与赖氨酸残基反应、保护蛋白质巯基或抑制葡萄糖与牛血清蛋白交联作用等抑制AGEs生成。展开更多
Adsorption and desorption of carbon dioxide, methane and other gases on coals has been investigated experimentally using representative Zhongliangshan coals. Gas adsorption is one of the major concerns for both CO2 se...Adsorption and desorption of carbon dioxide, methane and other gases on coals has been investigated experimentally using representative Zhongliangshan coals. Gas adsorption is one of the major concerns for both CO2 sequestration and methane recovery processes. The experiments were carried out using both single and multi-component mixtures at 25 ℃ and 30 ℃ with the highest pressure of 12 MPa. The coal was under moisture equilibrated conditions. This provides experimental data from which a predictive assessment of CO2 sequestration and/or methane recovery can be conducted. The results show that for pure gasses the CH4 adsorption capacity is higher than the N2 adsorption capacity but lower than the CO2 adsorption capacity. Injection of CO2 or other gases into the coal significantly affects CH4 desorption. This allows the enhancement of CH4 recovery from the coals, thus supplying more clean energy while sequestering significant amounts of CO2 thereby reducing the greenhouse effect from human beings.展开更多
文摘目的研究褐藻多酚对晚期糖基化终产物(advanced glycation end products,AGEs)生成的抑制作用及其机制。方法建立牛血清蛋白与葡萄糖体外模拟体系及食品热加工鱼肉肠模型,以不同浓度褐藻多酚为抑制剂,研究其对AGEs生成的抑制作用;通过分析模拟体系中赖氨酸和葡萄糖含量、蛋白质巯基水平及葡萄糖与牛血清蛋白的交联作用,阐明褐藻多酚对AGEs生成抑制作用机制。结果褐藻多酚对荧光性AGEs具有显著的抑制作用,100μg/g褐藻多酚反应36 h,对模拟体系AGEs生成抑制率达30.33%,50μg/mL褐藻多酚对鱼肉肠AGEs生成抑制率达41.91%。褐藻多酚处理后,模拟体系中赖氨酸和葡萄糖含量、蛋白质巯基水平显著高于对照组(P<0.05),而葡萄糖与牛血清蛋白交联条带量显著低于对照组(P<0.05)。结论褐藻多酚可能通过与赖氨酸残基反应、保护蛋白质巯基或抑制葡萄糖与牛血清蛋白交联作用等抑制AGEs生成。
基金Projects 02019 supported by the Key Project of Chinese Ministry of EducationARC by the Australian Research Council, 40730422+1 种基金2006AA06Z231 by the National Natural Science Foundation of China and Special Foundation of Cooperation NSFC-ARC08010202058 by the Anhui Province Key Project
文摘Adsorption and desorption of carbon dioxide, methane and other gases on coals has been investigated experimentally using representative Zhongliangshan coals. Gas adsorption is one of the major concerns for both CO2 sequestration and methane recovery processes. The experiments were carried out using both single and multi-component mixtures at 25 ℃ and 30 ℃ with the highest pressure of 12 MPa. The coal was under moisture equilibrated conditions. This provides experimental data from which a predictive assessment of CO2 sequestration and/or methane recovery can be conducted. The results show that for pure gasses the CH4 adsorption capacity is higher than the N2 adsorption capacity but lower than the CO2 adsorption capacity. Injection of CO2 or other gases into the coal significantly affects CH4 desorption. This allows the enhancement of CH4 recovery from the coals, thus supplying more clean energy while sequestering significant amounts of CO2 thereby reducing the greenhouse effect from human beings.
