The bushy root-2(brt-2)tomato mutant has twisting roots,and slower plant development.Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine(S75C)substitution in...The bushy root-2(brt-2)tomato mutant has twisting roots,and slower plant development.Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine(S75C)substitution in the DNA binding domain(DBD)of a heat shock factor class B(HsfB)encoded by SolycHsfB4a.This gene is orthologous to the Arabidopsis SCHIZORIZA gene,also known as AtHsfB4.The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered:a proliferation of lateral root cap and root meristematic tissues,and a tendency for lateral root cap cells to easily separate.The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations,but brt-2 is recessive.We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems.Since AtHsfB4 is induced by root knot nematodes(RKN),and loss-of-function mutants of this gene are resistant to RKNs,BRT-2 could be a target gene for RKN resistance,an important trait in tomato rootstock breeding.Gene&accession numbers SolycHsfB4a-Solyc04g078770.展开更多
基金The research was supported by BBSRC-UKRI fundingthe RootLINK(BB/L01954X/1)project focused on the“Understanding the Genetic Basis of Traits for Rootstock Improvement in Vegetable Crops”.
文摘The bushy root-2(brt-2)tomato mutant has twisting roots,and slower plant development.Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine(S75C)substitution in the DNA binding domain(DBD)of a heat shock factor class B(HsfB)encoded by SolycHsfB4a.This gene is orthologous to the Arabidopsis SCHIZORIZA gene,also known as AtHsfB4.The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered:a proliferation of lateral root cap and root meristematic tissues,and a tendency for lateral root cap cells to easily separate.The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations,but brt-2 is recessive.We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems.Since AtHsfB4 is induced by root knot nematodes(RKN),and loss-of-function mutants of this gene are resistant to RKNs,BRT-2 could be a target gene for RKN resistance,an important trait in tomato rootstock breeding.Gene&accession numbers SolycHsfB4a-Solyc04g078770.
