Cancer remains to be one of the most severe sicknesses globally.Cases have kept rising over the years.Breast cancer(BC),which is among the leading types of cancers and predominantly affects women,is the second leading...Cancer remains to be one of the most severe sicknesses globally.Cases have kept rising over the years.Breast cancer(BC),which is among the leading types of cancers and predominantly affects women,is the second leading cause of cancer mortality.Researchers have developed interventions over the years;however,the BC survival rate has not improved since the 1980s.This has created the need for novel drug interventions that would manage and treat BC more effectively.This study focused on using a combination of natural product extracts such as phytoestrogen(Ziziphus jujube)and Tannin nanoparticles(NP99)together,which we have referred to as(Z.NP99)and tamoxifen(Tam)as one of the leading BC drugs since the 70s,in treating BC.The effectiveness of Tam if used alone in the treatment and if combined with Z.NP99 was evaluated using MCF-7 cells in vitro.The findings showed that the combination treatment of Z.NP99 affected the proliferation and viability of MCF-7 cells more than Tam at a 10μg/mL dose.Moreover,Z.NP99 with Tam stimulated the maximum reduction of MCF-7 proliferation and viability in a time-dependent manner.Furthermore,Tam and Z.NP99 augmented the DNA fragmentation percentage combined with the upregulation of the apoptotic genes.Additionally,the results showed that the apoptotic impact of Z.NP99 and Tam on MCF-7 cells may be intermediated by down-regulating some genes such as Claudin-1 followed by down-regulating mRNA expression of MMP-9,VEGF,and BCL-2 genes of treated cells.Combining Tam with Z.NP99 considerably enhanced the effectiveness of conventional therapy.As a result,this study suggested that the Z.NP99 was ideal for developing effective natural treatments that would improve BC outcomes.展开更多
Recently,natural substances in the form of nanoparticles are increasingly being used in different field,particularly in medicines to enhance their beneficial effects in treatment and prevention.Cancer cells of the bre...Recently,natural substances in the form of nanoparticles are increasingly being used in different field,particularly in medicines to enhance their beneficial effects in treatment and prevention.Cancer cells of the breast(MCF-7)have been chosen to be examined and treated in vitro with conventional drug Tamoxifen(Tam)and tannin nanoparticles extract(NP99)individually or in combination.MTT reagent has been applied to assess the cell viability and propagation percentage,DNA fragmentation and mRNA relative expression of apoptotic genes to study the cell death pathway.The results showed that Tam and tannin NP99 triggered cytotoxic activity towards the MCF7 cell.They reduce the viability and induced high potent repressive activity on cell proliferation percentage and induced apoptosis as indicated by rising the percentage in DNA fragmentation.Effect of NP99 extract exhibited its effect in a dose and time-varying.The combined treatment of Tam and NP99 were much more efficient than individual drugs.It could be concluded that NP99 is considered a promising natural anticancer agent as a new tool in therapeutic strategies.展开更多
文摘Cancer remains to be one of the most severe sicknesses globally.Cases have kept rising over the years.Breast cancer(BC),which is among the leading types of cancers and predominantly affects women,is the second leading cause of cancer mortality.Researchers have developed interventions over the years;however,the BC survival rate has not improved since the 1980s.This has created the need for novel drug interventions that would manage and treat BC more effectively.This study focused on using a combination of natural product extracts such as phytoestrogen(Ziziphus jujube)and Tannin nanoparticles(NP99)together,which we have referred to as(Z.NP99)and tamoxifen(Tam)as one of the leading BC drugs since the 70s,in treating BC.The effectiveness of Tam if used alone in the treatment and if combined with Z.NP99 was evaluated using MCF-7 cells in vitro.The findings showed that the combination treatment of Z.NP99 affected the proliferation and viability of MCF-7 cells more than Tam at a 10μg/mL dose.Moreover,Z.NP99 with Tam stimulated the maximum reduction of MCF-7 proliferation and viability in a time-dependent manner.Furthermore,Tam and Z.NP99 augmented the DNA fragmentation percentage combined with the upregulation of the apoptotic genes.Additionally,the results showed that the apoptotic impact of Z.NP99 and Tam on MCF-7 cells may be intermediated by down-regulating some genes such as Claudin-1 followed by down-regulating mRNA expression of MMP-9,VEGF,and BCL-2 genes of treated cells.Combining Tam with Z.NP99 considerably enhanced the effectiveness of conventional therapy.As a result,this study suggested that the Z.NP99 was ideal for developing effective natural treatments that would improve BC outcomes.
基金This work was supported by the Deanship of Scientific Research at Taif University,Research Supporting Project No.TURSP-2020/76,Taif University,Taif,Saudi Arabia。
文摘Recently,natural substances in the form of nanoparticles are increasingly being used in different field,particularly in medicines to enhance their beneficial effects in treatment and prevention.Cancer cells of the breast(MCF-7)have been chosen to be examined and treated in vitro with conventional drug Tamoxifen(Tam)and tannin nanoparticles extract(NP99)individually or in combination.MTT reagent has been applied to assess the cell viability and propagation percentage,DNA fragmentation and mRNA relative expression of apoptotic genes to study the cell death pathway.The results showed that Tam and tannin NP99 triggered cytotoxic activity towards the MCF7 cell.They reduce the viability and induced high potent repressive activity on cell proliferation percentage and induced apoptosis as indicated by rising the percentage in DNA fragmentation.Effect of NP99 extract exhibited its effect in a dose and time-varying.The combined treatment of Tam and NP99 were much more efficient than individual drugs.It could be concluded that NP99 is considered a promising natural anticancer agent as a new tool in therapeutic strategies.
