Objective During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China. With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinic...Objective During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China. With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed. Methods Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3–10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. Results 502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426, with lower express volumes too. No Opa and Opc were detected in 053426. Conclusion The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.展开更多
A pulsating ultraluminous X-ray source(PULX)is a new kind of pulsar(PSR)whose characteristics are different from all known neutron stars.The magnetic field of PULX is suspected to be the main reason to support its sup...A pulsating ultraluminous X-ray source(PULX)is a new kind of pulsar(PSR)whose characteristics are different from all known neutron stars.The magnetic field of PULX is suspected to be the main reason to support its supper Eddington luminosity of PULX.NGC 7793 P13,which is the second confirmed PULX,can be easily studied due to its nearby position and isolation from other sources in its host galaxy.In this paper,we calculate its magnetic field to be∼1.0×10^(12) G based on the continued observations from 2016 to 2020.The magnetic field evolution of NGC 7793 P13 is analyzed,which shows that the source has spent about 10^(4) yr for the field decaying from the simulated initial strength 4.0×10^(14) G to the present value.In case of an assumed constant accretion and the limitation of the companion mass,it will be a recycled PSR whose magnetic field is ∼10^(9) G and spin period is a few hundred milliseconds.We estimate the field strength of the other confirmed PULXs and find main range is 10^(13)-10^(14) G.Their positions of the magnetic field and spin period are around or below the magnetars.This is because these PULXs are in the binary systems and are with the spin-up rate that are 2-3 orders higher than the normal binary pulsars.We suggest that PULXs are the accreting magnetars whose multi-pole strong magnetic field can support the supper Eddington luminosity.They would be helpful for studying the evolution of the magnetars,the formation of the binary PSRs above the Eddington spin-up line,and the millisecond PSRs with the magnetic field stronger than ∼10^(9) G.展开更多
基金supported by a grant (the Key Technologies R&D Program 2005BA711A09) from the Ministry of Science and Technology, of China
文摘Objective During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China. With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed. Methods Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3–10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. Results 502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426, with lower express volumes too. No Opa and Opc were detected in 053426. Conclusion The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.
基金supported by the National Natural Science Foundation of China(12130342,12273030 and U1938107)。
文摘A pulsating ultraluminous X-ray source(PULX)is a new kind of pulsar(PSR)whose characteristics are different from all known neutron stars.The magnetic field of PULX is suspected to be the main reason to support its supper Eddington luminosity of PULX.NGC 7793 P13,which is the second confirmed PULX,can be easily studied due to its nearby position and isolation from other sources in its host galaxy.In this paper,we calculate its magnetic field to be∼1.0×10^(12) G based on the continued observations from 2016 to 2020.The magnetic field evolution of NGC 7793 P13 is analyzed,which shows that the source has spent about 10^(4) yr for the field decaying from the simulated initial strength 4.0×10^(14) G to the present value.In case of an assumed constant accretion and the limitation of the companion mass,it will be a recycled PSR whose magnetic field is ∼10^(9) G and spin period is a few hundred milliseconds.We estimate the field strength of the other confirmed PULXs and find main range is 10^(13)-10^(14) G.Their positions of the magnetic field and spin period are around or below the magnetars.This is because these PULXs are in the binary systems and are with the spin-up rate that are 2-3 orders higher than the normal binary pulsars.We suggest that PULXs are the accreting magnetars whose multi-pole strong magnetic field can support the supper Eddington luminosity.They would be helpful for studying the evolution of the magnetars,the formation of the binary PSRs above the Eddington spin-up line,and the millisecond PSRs with the magnetic field stronger than ∼10^(9) G.
