Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, ...Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, EBAF, Thy-1, FGF4, Rex-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. Five of the six lines formed embryoid bodies that expressed markers of a variety of cell types; four of them formed teratomas with tissue types representative of all three embryonic germ layers. These human embryonic stem cells are capable of producing clones of undifferentiated morphology, and one of them was propagated to become a subline. Human embryonic stem cell lines from the Chinese population should facilitate stem cell research and may be valuable in studies of population genetics and ecology.展开更多
OBJECTIVE To examine the possible risk factors for stomach cancer among men and women in Shanghai, China. METHODS In urban Shanghai, in-person interviews were completed for 311 cases newly diagnosed with stomach cance...OBJECTIVE To examine the possible risk factors for stomach cancer among men and women in Shanghai, China. METHODS In urban Shanghai, in-person interviews were completed for 311 cases newly diagnosed with stomach cancer of ages 30-74. Data were collected from April 1999 to October 1999 and compared to 1579 population-based controls (controls in three cancer studies used together). Information on demographic variables, smoking, diet consumption and others was collected from all subjects. Unconditional logistic regression was used to adjust the possible factors. RESULTS Stomach cancer risk in men rose with smoking, eating hot and fried foods, chronic gastritis and a family history of gastric cancer among men; the risk among women was associated with the consumption of preserved,pickled and fried foods,heavy drinking, chronic gastritis, a history of ulcer disease and a family history of gastric cancer. A doseresponse relationship was found (trends test, P<0.01) among men smokers. In contrast, the consumption of fresh vegetables and fruits, beans (especially soybeans) and soy products, plant oil, and eggs and egg products, was inversely associated with stomach cancer risk. After adjustment of the potential confounding variables, these associations remained significant. CONCLUSIONS The present findings provide further evidence that smoking, eating salted foods (especially salted vegetables), oil-fried foods(including fried cereal, eggs, and peanuts), chronic gastritis, a family history of gastric cancer and so on increase the risk of stomach carcinoma in Shanghai. Fresh vegetables and fruits, beans and soybean products (even after adjusted for use of fresh vegetables and fruits), plant oil, and so on may have protective effects.展开更多
基金This work was supported by grants from National Ba-sic Research Program of China(973 Program)(No:001CB509903,001CB509904)Hi-Tech Research and Development Program of China(973 Program)(No:2001A A216121.2004AA205010)+3 种基金National Natural Science Foun-dation of China(No:30040003)Science and Technology Committee of Shanghai Municipality(No:99DJ 14002,00DJI 4033,0IDJ 14003.03DJ 14017)Chinese Academy of Science(No:KSCX-2-3-08)Shanghai Municipal Edu-cation Commission and Shanghai Second Medical University.
文摘Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, EBAF, Thy-1, FGF4, Rex-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. Five of the six lines formed embryoid bodies that expressed markers of a variety of cell types; four of them formed teratomas with tissue types representative of all three embryonic germ layers. These human embryonic stem cells are capable of producing clones of undifferentiated morphology, and one of them was propagated to become a subline. Human embryonic stem cell lines from the Chinese population should facilitate stem cell research and may be valuable in studies of population genetics and ecology.
文摘OBJECTIVE To examine the possible risk factors for stomach cancer among men and women in Shanghai, China. METHODS In urban Shanghai, in-person interviews were completed for 311 cases newly diagnosed with stomach cancer of ages 30-74. Data were collected from April 1999 to October 1999 and compared to 1579 population-based controls (controls in three cancer studies used together). Information on demographic variables, smoking, diet consumption and others was collected from all subjects. Unconditional logistic regression was used to adjust the possible factors. RESULTS Stomach cancer risk in men rose with smoking, eating hot and fried foods, chronic gastritis and a family history of gastric cancer among men; the risk among women was associated with the consumption of preserved,pickled and fried foods,heavy drinking, chronic gastritis, a history of ulcer disease and a family history of gastric cancer. A doseresponse relationship was found (trends test, P<0.01) among men smokers. In contrast, the consumption of fresh vegetables and fruits, beans (especially soybeans) and soy products, plant oil, and eggs and egg products, was inversely associated with stomach cancer risk. After adjustment of the potential confounding variables, these associations remained significant. CONCLUSIONS The present findings provide further evidence that smoking, eating salted foods (especially salted vegetables), oil-fried foods(including fried cereal, eggs, and peanuts), chronic gastritis, a family history of gastric cancer and so on increase the risk of stomach carcinoma in Shanghai. Fresh vegetables and fruits, beans and soybean products (even after adjusted for use of fresh vegetables and fruits), plant oil, and so on may have protective effects.
