酵母水解物对克氏原螯虾生长性能、消化、抗氧化和免疫能力的影响

实验研究了添加不同水平酵母水解物(Yeast hydrolysate,YHY)对克氏原螯虾(Procambarus clarkii)生长性能、消化、抗氧化和非特异性免疫能力的影响。实验选用300尾平均体重为(6.43±0.55)g的个体,随机分为5组,分别饲喂在基础饲料中添加0(对照组)、0.5%、1%、2%和4%YHY的饲料,每组3个重复,养殖密度为20尾/缸。为期8周的实验结束后,测定克氏原螯虾生长指标、胃和肝胰腺消化酶活性、肝胰腺抗氧化性能、血清生化指标和免疫酶活性。结果表明:(1)饲料中添加1%—4%YHY对该虾生长指标有促进作用。其中,2%添加组的存活率(SR)、最终体重(FBW)、最终体长(FBL)和肝胰腺指数(HSI)达最大值且显著高于对照组(P<0.05),增重率(WGR)和特定生长率(SGR)在1%添加组达到最大值且显著高于其余各组(P<0.05);(2)1%YHY添加组的全虾粗蛋白质含量显著高于其他组(P<0.05),而肌肉粗蛋白和粗脂肪含量在2%添加组达到最大值;(3)与对照组相比,2%—4%YHY添加组的胃和肝胰脏消化酶活性显著提高(P<0.05);(4)2%YHY添加组的肝胰腺抗氧化酶(过氧化氢酶,CAT;总超氧化物歧化酶,T-SOD;总抗氧化能力,T-AOC)和免疫酶(碱性磷酸酶,AKP;酸性磷酸酶,ACP)的活性达到最大值、丙二醛(MDA)的含量达到最小值,且与对照组相比均有显著差异(P<0.05);(5)2%YHY添加组该虾血清中总蛋白(TP)和白蛋白(ALB)含量显著高于其他组(P<0.05),1%和2%YHY添加组谷丙转氨酶(ALT)和谷草转氨酶(AST)活性显著低于其他各组(P<0.05)。结果表明,饲料中添加2%的酵母水解物可显著提高克氏原螯虾的消化、生长及肌肉粗蛋白和粗脂肪含量,并显著改善其抗氧化和非特异性免疫能力。

北斗坐标框架解算中的IGS参考站选站方法研究

首先从ITRF2020框架中筛选能接收北斗信号的站点;然后从数据完整性、卫星可见性、多路径误差等方面对站点观测数据质量进行分析,设定相应标准进行筛选;最后基于格网法提出一种既考虑均匀性又考虑数据质量和站点稳定性的选站方法。利用该方法优选72个全球均匀分布的测站作为北斗坐标框架解算中的IGS参考站。

哈利波特中国行主题创新科普实验——武汉站:化学魔法与现实世界的交融

本科普实验以青少年喜爱的魔幻文学人物哈利波特中国行为主题,以他的武汉之旅展开行程,用实验呈现博物馆的青铜器修复技术,“知音号”游轮的牺牲阳极法防腐技术,武汉长江大桥的水泥固化和桥头堡及桥墩的3D打印技术,黄鹤楼绘制的碳量子点制备技术;在潜移默化中传播化学知识。

基于新医科理念的“氧化还原反应和电极电位”实验改进

“新医科”是一种从治疗为主到兼具预防、治疗、康养的生命健康全周期的医学新理念。本文依据“新医科”理念,针对《医学基础化学实验》教材中的“氧化还原反应和电极电位”部分实验进行了“医学-化学-生物”等学科的交叉、融合与创新,具体有:(1)电池电动势的测量实验中用乙二胺四乙酸钠(Na4Y)代替浓氨水,实现样品体系中Cu^(2+)/Zn^(2+)浓度的变化;(2)氧化还原反应与电极电位的关系实验中用维生素C(Vc)的C_(6)H_(6)O_(6)(氧化型)/C_(6)H_(8)O_(6)(还原型)电对替代Br_(2)/Br^(-)电对,实现不同电对氧化还原性质的对比研究;(3)增设谷胱甘肽还原Fe^(3+)的实验,实现医学热点—细胞铁死亡过程的跨界思考。通过系列改进使实验变得绿色环保和医教结合,对诠释基础化学中的“新医科”理念具有重要意义。

地月历表误差对共线平动点周期轨道的误差影响分析

基于圆形限制性三体问题下地月平动点的3阶轨道解析解,分析不同初值条件下的轨道演化特性,并基于误差传播理论研究地月历表误差对地月共线平动点周期轨道在不同初值及初值误差时的影响。结果表明:1)轨道误差与地月历表误差在同一个量级,当地月历表误差均值不为0时,其峰值大约为地月历表误差的2.5倍,轨道误差随时间变化的无量纲周期约为轨道无量纲周期的0.5倍;2)在同一条轨道的不同位置,地月历表误差影响存在较大差异,平面Lyapunov轨道、Halo轨道和垂直Lyapunov轨道误差的峰值点分别位于或接近X向最大值与最小值处、Z向最大值与最小值处和z=0处。

Thermo-hydro-mechanical (THM) coupled simulation of the land subsidence due to aquifer thermal energy storage (ATES) system in soft soils

Aquifer thermal energy storage(ATES)system has received attention for heating or cooling buildings.However,it is well known that land subsidence becomes a major environmental concern for ATES projects.Yet,the effect of temperature on land subsidence has received practically no attention in the past.This paper presents a thermo-hydro-mechanical(THM)coupled numerical study on an ATES system in Shanghai,China.Four water wells were installed for seasonal heating and cooling of an agriculture greenhouse.The target aquifer at a depth of 74e104.5 m consisted of alternating layers of sand and silty sand and was covered with clay.Groundwater level,temperature,and land subsidence data from 2015 to 2017 were collected using field monitoring instruments.Constrained by data,we constructed a field scale three-dimensional(3D)model using TOUGH(Transport of Unsaturated Groundwater and Heat)and FLAC3D(Fast Lagrangian Analysis of Continua)equipped with a thermo-elastoplastic constitutive model.The effectiveness of the numerical model was validated by field data.The model was used to reproduce groundwater flow,heat transfer,and mechanical responses in porous media over three years and capture the thermo-and pressure-induced land subsidence.The results show that the maximum thermoinduced land subsidence accounts for about 60%of the total subsidence.The thermo-induced subsidence is slightly greater in winter than that in summer,and more pronounced near the cold well area than the hot well area.This study provides some valuable guidelines for controlling land subsidence caused by ATES systems installed in soft soils.

克氏原螯虾配合饲料黏合剂的黏合性能研究

为筛选出生产虾蟹类水产颗粒饲料用黏合剂,选择海藻酸钠、明胶、膨润土、羧甲基纤维素钠4种黏合剂,用于小批量制作克氏原螯虾(Procambarus clarkii)颗粒饲料,并评价了其耐水性,溶失率及对克氏原螯虾摄食率的影响。养殖实验结束后,对添加不同黏合剂的饲料在水中的稳定性和溶失率进行观察和计算,并且通过比较不同黏合剂对克氏原螯虾摄食率的影响,以此作为指标来筛选出适宜克氏原螯虾饲料的黏合剂。结果显示,四种黏合剂耐水时间分别为45′24″、31′53″、37′09″、32′26″,其中海藻酸钠的耐水性能最好,在水中溶失率最小(26.21%);明胶溶失率最大(58.42%)。经过7 d的投喂,发现用海藻酸钠作为黏合剂对克氏原螯虾的摄食率影响较小,适口性较好。研究结果表明,四种黏合剂在相同添加量的情况下,海藻酸钠耐水性能最好,溶失率最小,是实验饲料小批量生产条件下虾蟹类颗粒饲料比较适合的黏合剂。

Tuning Structural and Electronic Configuration of FeN_(4) via External S for Enhanced Oxygen Reduction Reaction

The Fe-N-C material represents an attractive oxygen reduction reaction electrocatalyst,and the FeN_(4)moiety has been identified as a very competitive catalytic active site.Fine tuning of the coordination structure of FeN_(4)has an essential impact on the catalytic performance.Herein,we construct a sulfur-modified Fe-N-C catalyst with controllable local coordination environment,where the Fe is coordinated with four in-plane N and an axial external S.The external S atom affects not only the electron distribution but also the spin state of Fe in the FeN_(4)active site.The appearance of higher valence states and spin states for Fe demonstrates the increase in unpaired electrons.With the above characteristics,the adsorption and desorption of the reactants at FeN_(4)active sites are optimized,thus promoting the oxygen reduction reaction activity.This work explores the key point in electronic configuration and coordination environment tuning of FeN_(4)through S doping and provides new insight into the construction of M-N-C-based oxygen reduction reaction catalysts.

Milestone progress of the HEPS booster commissioning

The high-energy photon source(HEPS)is the first fourth-generation synchrotron light source facility in China.The HEPS injector consists of a linear accelerator(Linac)and a full energy booster.The booster captures the electron beam from the Linac and increases its energy to the value required for the storage ring.The full-energy beam could be injected to the storage ring directly or after“high-energy accumulation.”On November 17,2023,the key booster parameters successfully reached their corresponding target values.These milestone results were achieved based on numerous contributions,including nearly a decade of physical design,years of equipment development and installation,and months of beam commissioning.As measured at the extraction energy of 6 GeV,the averaged beam current and emittance reached 8.57 mA with 5 bunches and 30.37 nm rad with a single-bunch charge of 5.58 nC,compared with the corresponding target values of 6.6 mA and 35 nm rad,respectively.This paper presents the physical design,equipment development,installation,and commissioning process of the HEPS booster.

Hepatic amyloidosis in a patient with chronic liver failure:A case report

BACKGROUND Amyloidosis is a rare disorder that can be classified into various types,and the most common type is the systemic light chain type.The prognosis of this disease is extremely poor.In general,amyloidosis mainly affects the kidneys and heart and manifests as abnormal proliferation of clonal plasma cells.Cases in which the liver is the primary organ affected by amyloidosis,as in this report,are less common in clinical practice.CASE SUMMARY A 62-year-old man was admitted with persistent liver dysfunction of unknown cause and poor treatment outcomes.His condition persisted,and he developed chronic liver failure,with severe cholestasis in the later stage that was gradually accompanied by renal injury.Ultimately,he was diagnosed with hepatic amyloidosis through liver biopsy and pathological examination.CONCLUSION Hepatic amyloidosis rarely occurs in the clinic,and liver biopsy and pathological examination can assist in the accurate and effective diagnosis of this condition.

Targeting CXCR4 and EDN1 for the treatment of recurrent miscarriage using stearic acid from traditional Chinese medicine

Background:Recurrent miscarriage(RM)affects an estimated 1-3%of couples attempting to conceive,and its molecular components stay ineffectively caught on.This study aims to explore potential therapeutic targets for RM by examining gene expression patterns and biological pathways in both mouse and human RM models.Meanwhile,explore relevant traditional Chinese medicine(TCM)components targeting potential therapeutic targets.Methods:We utilized the GSE211251 mouse and the GSE26787 human datasets,employing gene set enrichment analysis and gene metaphysics analysis to examine differentially expressed genes and enriched pathways.Single-cell RNA analysis uncovered cellular heterogeneity and arranged pharmacology-mapped potential drug-target intelligence.We employed molecular docking strategies to assess the affinity of TCM components for key proteins.Results:In the mouse model,genes such as Ly6f1 and Gpr26 were upregulated,while Stc5a and Galca exhibited downregulation.Gene set enrichment analysis identified key pathways,including the tumor necrosis factor-mediated signaling pathway.In human samples,Gene Ontology analysis highlighted processes such as apoptosis and cell adhesion.Single-cell RNA analysis revealed distinct cellular populations between normal and RM samples.Systems pharmacology identified C-X-C motif chemokine receptor 4(CXCR4)and endothelin 1(EDN1)as potential key targets,and molecular docking confirmed that stearic acid from TCM appears to regulate these proteins.Conclusion:This study presents a comprehensive analysis of the genetic and cellular underpinnings of RM,identifying CXCR4 and EDN1 as promising therapeutic targets.Stearic acid from TCM could provide targeted treatment by modulating these key proteins,paving the way for new RM treatment strategies.

Penetrating canaloplasty in corticosteroid-induced glaucoma:a report of two cases

Dear Editor,We have treated two patients of glucocorticoid-induced glaucoma with penetrating canaloplasty(PCP)since 2021.PCP is a new type internal drainage surgery for glaucoma,which is a bleb-independent filtering surgery combined canaloplasty with trabeculectomy,and the surgery shows a great efficacy of intraocular pressure(IOP)reduction with no surgical complication.PCP surgery started with a fornix-based conjunctival flap and double scleral flap,and Schlemm’s canal was opened,a 10-0 prolene suture was positioned and tensioned within Schlemm’s canal,trabucular meshwork segment was excised and peripheral iridectomy was performed,the scleral flap and the conjunctival flap were closed watertight with 10-0 prolene sutures.

Global research trends in postoperative ileus from 2011 to 2023:A scientometric study

BACKGROUND Postoperative ileus(POI)is a common complication after abdominal surgery with high morbidity,which hinders patient recovery,prolongs hospitalization,and increases healthcare costs.Therefore,POI has become a global public health challenge.POI triggering is multifactorial.Autonomic and hormonal mechanisms are generally involved in POI pathogenesis.Recent studies have shown that beta adrenergic signaling of enteric glia is a POI trigger.Currently,the status quo,trends,and frontiers of global research on POI remain unclear.AIM To explore the current status,trends,and frontiers of POI research from 2011 to the present based on bibliometric analysis.METHODS Publications published on POI research from 2011 to 2023 were retrieved on June 1,2023,from the Web of Science Core Collection.CiteSpace 6.2.R2 and VOSviewer were used to conduct bibliometric visualization.RESULTS In total,778 POI records published from 2011 to 2023 were retrieved.Over the past few decades,the annual cumulative number of related articles has linearly increased,with China and the United States of America contributing prominently.All publications were from 59 countries and territories.China and the University of Bonn were the top contributing country and institution,respectively.Neurogastroenterology&Motility was the most prolific journal.The Journal of Gastrointestinal Surgery had the highest number of citations.Wehner Sven was the most productive author.Burst keywords(e.g.,colon,prolonged ileus,acupuncture,paralytic ileus,pathophysiology,rectal cancer,gastrointestinal function,risk)and a series of reference citation bursts provided evidence for the research frontiers in recent years.CONCLUSION This study demonstrates trends in the published literature on POI and provides new insights for researchers.It emphasizes the importance of multidisciplinary cooperation in the development of this field.

Effect of inflammatory response on joint function after hip fracture in elderly patients:A clinical study

BACKGROUND Excellent hip joint function facilitates limb recovery and improves the quality of survival.This study aimed to investigate the potential risk factors affecting postoperative joint functional activity and outcomes in elderly hip fractures patients and to provide evidence for patient rehabilitation and clinical management.AIM To explore the relationship between inflammatory factors and hip function and the interaction between inflammation and health after hip fracture in elderly patients.METHODS The elderly patients who had hip fracture surgery at our hospital between January 1,2021,and December 31,2022 were chosen for this retrospective clinical investigation.Patients with excellent and fair postoperative hip function had their clinical information and characteristics gathered and compared.Age,gender,fracture site,surgical technique,laboratory indices,and other variables that could have an impact on postoperative joint function were all included in a univariate study.To further identify independent risk factors affecting postoperative joint function in hip fractures,risk factors that showed statistical significance in the univariate analysis were then included in a multiple logistic regression analysis.In addition to this,we also compared other outcome variables such as visual analogue scale and length of hospital stay between the two groups.RESULTS A total of 119 elderly patients with hip fractures were included in this study,of whom 37 were male and 82 were female.The results of univariate logistic regression analysis after excluding the interaction of various factors showed that there was a statistically significant difference in interleukin(IL)-6,IL-8,IL-10,C-reactive protein(CRP),and complement C1q(C1q)between the fair and excellent joint function groups(P<0.05).The results of multiple logistic regression analysis showed that IL-6>20 pg/mL[Odds ratio(OR)3.070,95%CI:1.243-7.579],IL-8>21.4 pg/mL(OR 3.827,95%CI:1.498-9.773),CRP>10 mg/L(OR 2.142,95%CI:1.020-4.498)and C1q>233 mg/L(OR 2.339,95%CI:1.094-5.004)were independent risk factors for poor joint function after hip fracture surgery(all P<0.05).CONCLUSION After hip fractures in older patients,inflammatory variables are risk factors for fair joint function;therefore,early intervention to address these markers is essential to enhance joint function and avoid consequences.

Animal Safety Test of Bacillus thuringiensis BT Protein

[Objectives]To determine the biological safety of BT protein from Bacillus thuringiensis(Bt)fermentation broth to mammals at high doses.[Methods]Healthy mice were randomly divided into 4 groups with 10 mice in each group.The experimental groups were fed with Bt fermentation supernatant at 0.2,0.6 and 1.0 mL/kg,respectively,once a day for 7 consecutive days.The blank control group was fed normally without intragastric administration.[Results]There was no significant difference in blood routine and blood biochemical analysis between the experimental group and the control group.After intragastric administration,the mice were dissected,and no obvious pathological changes were found;the heart,liver,spleen,lung and kidney were taken to make tissue sections,and no pathological changes were found by microscopic observation.[Conclusions]Mice can tolerate high doses of BT protein from B.thuringiensis fermentation broth.

Development of an Information-Based Leadership Evaluation Index System for School Management Teams:A Comprehensive Approach

In the context of educational informatization reform,there are many uncertain factors in the process of education development.To optimize the educational environment so that it can be effectively combined with information technology,teachers need to change their teaching ideas,strengthen the school’s own adaptability to informatization,and promote the development of informatization construction in schools.As a branch of information leadership,the management team should establish an evaluation index system for information leadership in order to ensure the construction of school informatization,so as to provide a boost for the integration of school resources and information technology.Based on the overview and connotation of information-based leadership of the management team,this paper explores the construction of an evaluation index system of information-based leadership of the school management team,aiming to provide references for the research of relevant personnel in the future.

Analysis of Information Leadership and Digital Campus Management Strategy

In the information age,the development of schools needs to be integrated with information education,and the principal,as the leader of the school,has a positive significance for the development of the school.Therefore,in the process of school informatization development,principals should continuously improve their informatization leadership,and take digitalization as the guide to build schools,accelerate the construction and improvement of campus management,and then promote the school to continuously optimize the overall management and business processes under the immersion of digital culture.Based on this,this paper is based on information leadership and digital campus,and explores the way to improve the information leadership of principals based on the construction of digital campus.

发酵饲料对克氏原螯虾生长性能、肌肉品质、抗氧化能力和肠道微生物群落的影响

为探究克氏原螯虾配合饲料经发酵处理后的饲喂效果,分别用未发酵和发酵饲料在室内循环水系统中饲养体重为(4.91±0.18)g的克氏原螯虾幼虾8周,采样分析发酵饲料对克氏原螯虾生长、肌肉品质、消化力、抗氧化能力及肠道菌群结构的影响。结果显示,①发酵饲料水中溶失率显著低于未发酵饲料;②发酵饲料显著提高克氏原螯虾增重率和特定生长率,降低饲料系数,但对存活率、肝体比、含肉率和体成分无显著影响;③发酵饲料显著提高克氏原螯虾肌肉硬度、弹性、咀嚼性和胶黏性,降低了黏附性;④发酵饲料显著提高克氏原螯虾肝胰腺及肠道中胰蛋白酶和脂肪酶活性,血清和肝胰腺中超氧化物歧化酶、酸性磷酸酶和碱性磷酸酶活性,以及肝胰腺总抗氧化能力;⑤发酵饲料显著提高了肠道绒毛长度和宽度;⑥发酵饲料改善了克氏原螯虾肠道微生物结构,显著升高了厚壁菌门和放线菌门相对丰度,同时提高了肠道菌群多样性。研究表明,饲料经发酵处理后可以有效提高克氏原螯虾生长性能、肌肉品质、消化力和抗氧化能力,改善了肠道组织结构,改变了肠道微生物结构,为克氏原螯虾新型环保饲料的生产提供理论依据。

Optical potential approach for positron scattering by metastable 2~3S state of helium

The momentum space coupled channels optical（CCO） method for positron scattering has been extended to study the scattering of positrons by metastable helium for impact energies in the range from the positronium threshold up to high energies. Both the positronium formation and ionization continuum channels are included in the calculations via a complex equivalent local potential. The positronium formation, ionization, elastic and 2~3S–2~3P excitation, and total scattering cross sections are all presented and compared with the available information.

基于PERMA模式的积极心理干预对癌症患者主观幸福感和负性情绪影响的Meta分析

目的:系统评价基于PERMA模式的积极心理干预对癌症患者主观幸福感及负性情绪的干预效果。方法:计算机检索中国知网、万方数据库、维普数据库、the Cochrane Library、PubMed及Web of Science数据库中PERMA模式对癌症患者干预效果的随机对照试验,检索时限自建库至2022年7月30日。使用RevMan 5.3软件进行统计分析。结果:共纳入10篇文献,共1005例研究对象。Meta分析结果显示,与常规护理相比,PERMA干预能提高患者的主观幸福感[SMD=1.54,95%CI(1.05,2.03),P<0.001],缓解癌症患者的焦虑[SMD=-1.12,95%CI(-1.76,-0.48),P<0.001]、抑郁[SMD=-2.34,95%CI(-3.60,-1.09),P<0.001]和癌症复发恐惧[SMD=-1.33,95%CI(-1.60,-1.06),P<0.001]。结论:基于PERMA模式的积极心理干预能够降低癌症患者的负性情绪和癌症复发恐惧,提高主观幸福感。