该文构建一种基于靶标诱导滚环扩增(rolling circle amplification,RCA)的无标记适配体快速检测赭曲霉毒素A(ochratoxin A,OTA)生物传感器。该生物传感器探针由RCA引物与OTA适配体两部分组成,在OTA存在的环境中,OTA适配体特异性识别靶标...该文构建一种基于靶标诱导滚环扩增(rolling circle amplification,RCA)的无标记适配体快速检测赭曲霉毒素A(ochratoxin A,OTA)生物传感器。该生物传感器探针由RCA引物与OTA适配体两部分组成,在OTA存在的环境中,OTA适配体特异性识别靶标,探针结构被打开,RCA引物与环状DNA模板(circular DNA template,CT)结合开启RCA反应,加入核酸染料SYBR Gold产生荧光信号。此生物传感器具有较高的特异性,检测限为6.6×10^(-2)nmol/L,线性检测范围为6.6×10^(-2)~660 nmol/L,可用于具体的分析检测。此生物传感器无需复杂化学修饰且操作简单,在食品安全检测中具有良好的应用前景。展开更多
煤矿坑道钻机自动化和智能化程度的提高、作业范围和应用场景的扩展,使其系统复杂性和工作环境恶劣程度随之增加,对煤矿坑道钻机可靠性提出新的挑战。为有效降低因钻机故障带来的安全风险,提升钻机全寿命周期的任务品质,煤矿坑道钻机状...煤矿坑道钻机自动化和智能化程度的提高、作业范围和应用场景的扩展,使其系统复杂性和工作环境恶劣程度随之增加,对煤矿坑道钻机可靠性提出新的挑战。为有效降低因钻机故障带来的安全风险,提升钻机全寿命周期的任务品质,煤矿坑道钻机状态监测与故障诊断技术应运而生。首先从钻机状态参数采集、数据处理、诊断方法、系统应用等四个方面对煤矿坑道钻机关键系统状态监测和故障诊断技术研究现状进行总结分析,然后在故障预测与健康管理(prognostics and health management, PHM)技术基本概念的基础上根据集中式与分布式结合的方式提出了坑道钻机PHM技术框架,最后对煤矿坑道钻机PHM技术发展趋势进行展望,旨在为新一代煤矿坑道钻机及相关煤矿机械装备PHM技术的研究开展提供参考和借鉴。展开更多
Objective: To explore graduation practical ability assessment mode of graduate students in nursing (Master of Nursing Specialist, MNS). Methods: All 28 MNS 2020th graduate students in a university were selected as sub...Objective: To explore graduation practical ability assessment mode of graduate students in nursing (Master of Nursing Specialist, MNS). Methods: All 28 MNS 2020th graduate students in a university were selected as subjects by convenient sampling method, using the improved 4-station objective structured examination (Objective Structured Clinical Examination, OSCE) model. The assessment content included nursing assessment, nursing diagnosis, basic nursing operation, specialist nursing operation, theoretical written test and oral test and overall evaluation. And self-made questionnaire was used to evaluate the effect of the assessment model and the improvement of clinical practice ability of graduate students. Results: The overall satisfaction rate of graduation assessment was 89.51%, the overall satisfaction rate of graduation assessment mode and practical ability improvement evaluation was 84.66%, the pass rate of assessment results was 100%, and the excellent rate was 71.42%. Conclusion: The MNS graduation practical ability assessment model has a high degree of satisfaction, objectively evaluates the knowledge, skills, attitudes and emotions of graduate students, promotes the comprehensive quality and ability of graduate students, which is worthy of promotion.展开更多
Objective: To explore the application effect of case combined with seminar teaching method in Pediatric Nursing. Methods: Two classes in junior year were selected by convenience sampling in 2022, and the two classes w...Objective: To explore the application effect of case combined with seminar teaching method in Pediatric Nursing. Methods: Two classes in junior year were selected by convenience sampling in 2022, and the two classes were randomly divided into the intervention group and control group by tossing a coin. The intervention group utilized the case combined with seminar teaching method, while the control group used the traditional teaching-based method. The stage test average score, final exam results were compared after the end of the course. The autonomous learning ability evaluation scale and the Chinese critical thinking ability scale were used to attain students’ autonomous learning ability and critical thinking ability before and after the course. The self-made evaluation scale was used to know students’ evaluation of the new teaching method after the course. Results: 1) The stage test average scores of the intervention group and the control group were 79.13 ± 6.39 and 74.67 ± 5.25 respectively (P , the final exam scores were 79.83 ± 6.09 and 75.21 ± 4.91 respectively (P < 0.05);2) The total scores of autonomous learning ability were 118.67 ± 7.70 and 106.49 ± 7.67 respectively (P , and the total scores of critical thinking ability were 283.45 ± 7.82 and 251.37 ± 8.93 respectively (P < 0.05);3) More than 80% of the students in the intervention group believed that the case combined with seminar teaching method could deepen the understanding of knowledge, promote the habit of thinking and literature consulting, and improve the ability to analyze and solve problems, integrate knowledge and communicate and coordinate. Conclusions: Application of case combined with seminar teaching method in Pediatric Nursing improved the students’ autonomous learning ability and critical thinking ability, and strengthened the students’ understanding and application of knowledge. The students had a positive attitude towards the new teaching method.展开更多
文摘该文构建一种基于靶标诱导滚环扩增(rolling circle amplification,RCA)的无标记适配体快速检测赭曲霉毒素A(ochratoxin A,OTA)生物传感器。该生物传感器探针由RCA引物与OTA适配体两部分组成,在OTA存在的环境中,OTA适配体特异性识别靶标,探针结构被打开,RCA引物与环状DNA模板(circular DNA template,CT)结合开启RCA反应,加入核酸染料SYBR Gold产生荧光信号。此生物传感器具有较高的特异性,检测限为6.6×10^(-2)nmol/L,线性检测范围为6.6×10^(-2)~660 nmol/L,可用于具体的分析检测。此生物传感器无需复杂化学修饰且操作简单,在食品安全检测中具有良好的应用前景。
文摘煤矿坑道钻机自动化和智能化程度的提高、作业范围和应用场景的扩展,使其系统复杂性和工作环境恶劣程度随之增加,对煤矿坑道钻机可靠性提出新的挑战。为有效降低因钻机故障带来的安全风险,提升钻机全寿命周期的任务品质,煤矿坑道钻机状态监测与故障诊断技术应运而生。首先从钻机状态参数采集、数据处理、诊断方法、系统应用等四个方面对煤矿坑道钻机关键系统状态监测和故障诊断技术研究现状进行总结分析,然后在故障预测与健康管理(prognostics and health management, PHM)技术基本概念的基础上根据集中式与分布式结合的方式提出了坑道钻机PHM技术框架,最后对煤矿坑道钻机PHM技术发展趋势进行展望,旨在为新一代煤矿坑道钻机及相关煤矿机械装备PHM技术的研究开展提供参考和借鉴。
文摘Objective: To explore graduation practical ability assessment mode of graduate students in nursing (Master of Nursing Specialist, MNS). Methods: All 28 MNS 2020th graduate students in a university were selected as subjects by convenient sampling method, using the improved 4-station objective structured examination (Objective Structured Clinical Examination, OSCE) model. The assessment content included nursing assessment, nursing diagnosis, basic nursing operation, specialist nursing operation, theoretical written test and oral test and overall evaluation. And self-made questionnaire was used to evaluate the effect of the assessment model and the improvement of clinical practice ability of graduate students. Results: The overall satisfaction rate of graduation assessment was 89.51%, the overall satisfaction rate of graduation assessment mode and practical ability improvement evaluation was 84.66%, the pass rate of assessment results was 100%, and the excellent rate was 71.42%. Conclusion: The MNS graduation practical ability assessment model has a high degree of satisfaction, objectively evaluates the knowledge, skills, attitudes and emotions of graduate students, promotes the comprehensive quality and ability of graduate students, which is worthy of promotion.
文摘Objective: To explore the application effect of case combined with seminar teaching method in Pediatric Nursing. Methods: Two classes in junior year were selected by convenience sampling in 2022, and the two classes were randomly divided into the intervention group and control group by tossing a coin. The intervention group utilized the case combined with seminar teaching method, while the control group used the traditional teaching-based method. The stage test average score, final exam results were compared after the end of the course. The autonomous learning ability evaluation scale and the Chinese critical thinking ability scale were used to attain students’ autonomous learning ability and critical thinking ability before and after the course. The self-made evaluation scale was used to know students’ evaluation of the new teaching method after the course. Results: 1) The stage test average scores of the intervention group and the control group were 79.13 ± 6.39 and 74.67 ± 5.25 respectively (P , the final exam scores were 79.83 ± 6.09 and 75.21 ± 4.91 respectively (P < 0.05);2) The total scores of autonomous learning ability were 118.67 ± 7.70 and 106.49 ± 7.67 respectively (P , and the total scores of critical thinking ability were 283.45 ± 7.82 and 251.37 ± 8.93 respectively (P < 0.05);3) More than 80% of the students in the intervention group believed that the case combined with seminar teaching method could deepen the understanding of knowledge, promote the habit of thinking and literature consulting, and improve the ability to analyze and solve problems, integrate knowledge and communicate and coordinate. Conclusions: Application of case combined with seminar teaching method in Pediatric Nursing improved the students’ autonomous learning ability and critical thinking ability, and strengthened the students’ understanding and application of knowledge. The students had a positive attitude towards the new teaching method.
