Pain on the body surface might accompany disorders in the deep tissue or internal organ.However,the anatomical and physiological mechanism is obscure.Here,we provided direct evidences of axon bifurcation in primary C-...Pain on the body surface might accompany disorders in the deep tissue or internal organ.However,the anatomical and physiological mechanism is obscure.Here,we provided direct evidences of axon bifurcation in primary C-nociceptive neurons that innervate both the skin and a visceral organ.Double-labeled dorsal root ganglion(RG)neurons and Evans blue extravasation were observed in 3 types of chemo-induced visceral inflammation(colitis,urocystitis and acute gastritis)rat models.In the rat model of colitis,mechanical hypersensitivity and spontaneous activities were recorded in vivo from double-labeled C nociceptive neurons in S1 or L6 DRG.These neurons showed significantly enhanced responses to both somatic stimulation and colorectal distension.Our findings suggest that axon branching of C-noceptors may contribute to cutaneous hypersensitivity in visceral inflammation.Cutaneous hypersensitivity on certain locations of body surface might serve as an indicator of pathological conditions in the corresponding visceral organ.展开更多
We investigate the morphological and electrophysiological mechanism of gate control for pain.We proposed that the regulation of peripheral A-fibers on C fibers was mediated by the GABAergic interneurons in the spinal ...We investigate the morphological and electrophysiological mechanism of gate control for pain.We proposed that the regulation of peripheral A-fibers on C fibers was mediated by the GABAergic interneurons in the spinal cord.The cobra venom was injected to the sciatic nerve to establish a neuropathic pain model due to peripheral demyelination within A-fiber.The content of r aminobutyric acid(GABA)in spinal cord and cerebrospinal fluid was asssed by high performance liquid chromatography(HPLC).The microstructure of A-fiber,C-fiber and the GABAergic neurons in the spinal cord was observed by immunofluorescence staining.展开更多
Bupivacaine encapsulated poly(lactidecoglycolide)nanoparticles is a novel formulation of extended-release of bupivacaine in poly(lactidecoglycolide)matrix.We evaluated the efficacy and safety of bupivacaine nanopartic...Bupivacaine encapsulated poly(lactidecoglycolide)nanoparticles is a novel formulation of extended-release of bupivacaine in poly(lactidecoglycolide)matrix.We evaluated the efficacy and safety of bupivacaine nanoparticles in peripheral analgesia via behavior tests,electrophysiological recordings,pharmacokinetic analysis,and pathology staining by employing the models of uninjured rats and mice.Nanoparticles injection increased the pain thresholds of rats and mice for 21 days.展开更多
文摘Pain on the body surface might accompany disorders in the deep tissue or internal organ.However,the anatomical and physiological mechanism is obscure.Here,we provided direct evidences of axon bifurcation in primary C-nociceptive neurons that innervate both the skin and a visceral organ.Double-labeled dorsal root ganglion(RG)neurons and Evans blue extravasation were observed in 3 types of chemo-induced visceral inflammation(colitis,urocystitis and acute gastritis)rat models.In the rat model of colitis,mechanical hypersensitivity and spontaneous activities were recorded in vivo from double-labeled C nociceptive neurons in S1 or L6 DRG.These neurons showed significantly enhanced responses to both somatic stimulation and colorectal distension.Our findings suggest that axon branching of C-noceptors may contribute to cutaneous hypersensitivity in visceral inflammation.Cutaneous hypersensitivity on certain locations of body surface might serve as an indicator of pathological conditions in the corresponding visceral organ.
文摘We investigate the morphological and electrophysiological mechanism of gate control for pain.We proposed that the regulation of peripheral A-fibers on C fibers was mediated by the GABAergic interneurons in the spinal cord.The cobra venom was injected to the sciatic nerve to establish a neuropathic pain model due to peripheral demyelination within A-fiber.The content of r aminobutyric acid(GABA)in spinal cord and cerebrospinal fluid was asssed by high performance liquid chromatography(HPLC).The microstructure of A-fiber,C-fiber and the GABAergic neurons in the spinal cord was observed by immunofluorescence staining.
文摘Bupivacaine encapsulated poly(lactidecoglycolide)nanoparticles is a novel formulation of extended-release of bupivacaine in poly(lactidecoglycolide)matrix.We evaluated the efficacy and safety of bupivacaine nanoparticles in peripheral analgesia via behavior tests,electrophysiological recordings,pharmacokinetic analysis,and pathology staining by employing the models of uninjured rats and mice.Nanoparticles injection increased the pain thresholds of rats and mice for 21 days.
