Plant height is an important morphological trait that affects crop yield.Several genes related to plant height and yield have been reported in rice(Oryza sativa L.),however,the molecular mechanism underlying the regul...Plant height is an important morphological trait that affects crop yield.Several genes related to plant height and yield have been reported in rice(Oryza sativa L.),however,the molecular mechanism underlying the regulation of these traits is still not completely understood.VP64 is widely used as a transcriptional activator to investigate the biological function of genes encoding transcription factors.Here,we identified a novel bZIP transcription factor OsbZIP62 that is involved in modulating agronomic traits in rice.Overexpression of OsbZIP62-VP64(OsbZIP62V)significantly increases the plant height and yield per plant in rice.RNA-seq analysis showed that some plant height and panicle development related genes(i.e.,OsEATB,OsDSS1 and OsGA3ox2)were up-regulated in OsbZIP62V overexpressing rice plants.Besides,OsbZIP62 could also bind to the promoters of several putative target genes.These results suggested that OsbZIP62 plays a role as transcriptional regulator in regulating the expression of genes associated with agronomic traits,and OsbZIP62 fused with VP64 would be useful in crop genetic modification with improved plant architecture and yield.展开更多
The increasing concentration of greenhouse gases(GHGs)in Earth's atmosphere leads to global warming,which further causes a series of climate changes and does great harm to both human society and natural ecosystems...The increasing concentration of greenhouse gases(GHGs)in Earth's atmosphere leads to global warming,which further causes a series of climate changes and does great harm to both human society and natural ecosystems.Agricultural GHG emissions,mainly in theform of methane(CH4)and nitrous oxide(N2O),areasignificantsourceofGHGs,accountingfor~14%total global GHGs(Zhang et al.,2022).One major source of agricultural GHGs is CH4 emissions from rice paddies,which is responsiblefor~10%-12%ofhuman-inducedCH4emissions(van Groenigen et al.,2013)and contributes~2.40%to the enhanced global warming effect(Zhang et al.,2022).The global warming potential of GHGs emissions from rice systems is roughly four times higher than either wheat or maize(Linquist et al.,2012).展开更多
基金Funding for Basic Research Projects of Taicang(TC2021JC12)School-Level Research Projects of Yancheng Institute of Technology(xjr2021029)+1 种基金Shanghai Agriculture Applied Technology Development Program(2017-02-08-00-08-F00071)Natural Science Foundation of Shanghai(18ZR1433300)。
文摘Plant height is an important morphological trait that affects crop yield.Several genes related to plant height and yield have been reported in rice(Oryza sativa L.),however,the molecular mechanism underlying the regulation of these traits is still not completely understood.VP64 is widely used as a transcriptional activator to investigate the biological function of genes encoding transcription factors.Here,we identified a novel bZIP transcription factor OsbZIP62 that is involved in modulating agronomic traits in rice.Overexpression of OsbZIP62-VP64(OsbZIP62V)significantly increases the plant height and yield per plant in rice.RNA-seq analysis showed that some plant height and panicle development related genes(i.e.,OsEATB,OsDSS1 and OsGA3ox2)were up-regulated in OsbZIP62V overexpressing rice plants.Besides,OsbZIP62 could also bind to the promoters of several putative target genes.These results suggested that OsbZIP62 plays a role as transcriptional regulator in regulating the expression of genes associated with agronomic traits,and OsbZIP62 fused with VP64 would be useful in crop genetic modification with improved plant architecture and yield.
基金funded by the Shanghai Agriculture Applied Technology Development Program(T20210104 and G2016060301)the National Key Research and Development Program of China(2018YFE0106200)the Shanghai Natural Science Foundation(20ZR1449300).
文摘The increasing concentration of greenhouse gases(GHGs)in Earth's atmosphere leads to global warming,which further causes a series of climate changes and does great harm to both human society and natural ecosystems.Agricultural GHG emissions,mainly in theform of methane(CH4)and nitrous oxide(N2O),areasignificantsourceofGHGs,accountingfor~14%total global GHGs(Zhang et al.,2022).One major source of agricultural GHGs is CH4 emissions from rice paddies,which is responsiblefor~10%-12%ofhuman-inducedCH4emissions(van Groenigen et al.,2013)and contributes~2.40%to the enhanced global warming effect(Zhang et al.,2022).The global warming potential of GHGs emissions from rice systems is roughly four times higher than either wheat or maize(Linquist et al.,2012).
