Expression and clinical significance of serum lipoprotein(a) in patients with gastric cancer

Objective This study aimed to investigate the expression and clinical significance of serum lipoprotein(a) [LP(a)] in patients with gastric cancer. Methods Two hundred and twenty-two patients with gastric cancer(gastric cancer group) were selected from 2015 to 2017 [mean age(58.40 ± 10.40) years], as were 101 healthy persons [normal age group, mean age(58.18 ± 11.42) years]. Fasting blood samples were collected and evaluated by immunoturbidimetry with a biochemical analyzer. LP(a) concentration was observed and its difference was compared. Results There was no significant correlation between LP(a) and tumor stage(P > 0.05). Compared with the control group, the level of LP(a) in the male gastric cancer group was significantly higher than that in the control group(P < 0.05). In the subgroup analysis, the level of LP(a) and abnormal rate showed an increasing trend among patients with stages I–IV gastric cancer. The level of LP(a) in poorly differentiated gastric cancer patients was higher than that in the high middle differentiation group(P < 0.05). There was no significant difference in LP(a) levels among patients with different pathological types of gastric cancer(P > 0.05). Conclusion LP(a) was correlated with the occurrence, development and differentiation of gastric cancer, but not with the pathological classification of gastric cancer. Serum LP(a) concentration may be used as an indicator for the staging and prognosis of gastric cancer, but the specific underlying mechanism remains to be further studied.

Effects of transfecting Bcl-2 shRNA and celecoxb on the SGC-7901 cell line

Objective:The aim of this study was to study the changes of SGC-7901 cells transfecting small hairpin RNA(shRNA) targeted Bcl-2 and celecoxib in vitro.Methods:To use the effective siRNA targeted to Bcl-2 by Lipofectamine 2000,the rate of cell growth inhibition of all groups was detected by multiply-table tournament(MTT) method,apoptosis rate was examined by flow cytometry and the expression of Bcl-2 was assayed by Elisa.Results:After siRNA was transfected to SGC-7901 cells,the rate of cell growth inhibition was increased,the joint group was higher by flow cytometry and the expression of Bcl-2 was lower by Elisa.Conclusion:The growth of SGC-7901 cells which was transfected siRNA has been inhibited,and the sensitivity to celecoxib has been increased.

Vasculogenic mimicry in non-small cell lung cancer and its relationship with tumor stage

Objective： The purpose of the study was to study the mechanism of vasculogenic mimicry （VM） and its relationship with tumor stage in non-small cell lung cancer （NSCLC）. Methods： Forty-two patients with NSCLC were collected, 19 belonged to the early stage （stages Ⅰ ＋Ⅱ） while 23 were late stage （stages Ⅲ ＋ Ⅳ）. Moreover, 20 patients got surgical treat ment and 22 got chemotherapy. We studied the relationship of VM with stage, chemotherapeutic effect, HIF-la, microves sel density （MVD） and clinicopathologic features. Results： VM in patients of early stages were significantly more than late stages （68.4% vs 26.1%, P = 0.006）, and the positive rate of VM was proportional to HIF-la （P = 0.034）. But no correlation was found between VM and chemotherapeutic effect （14.3% vs 26.7%, P = 1.00） or MVD （P 〉 0.05）. Furthermore, we found VM also showed a negative correlation with distant metastases and lymph nodes metastases （P 〈 0.05） while no correlation was found with other clinicopathologic. Conclusion： VM was generated during the early stage in NSCLC and correlated with lymph nodes metastases. As the disease progressed, VM may be replaced by vascular endothelial cells, so the late-stage patients especially people with distant metastases had fewer VM. As the main factor produced by hypoxia, HIF-la may make a difference in VM formation. Thus we inferred VM might be a new target for targeted therapy, and could provide help for clinical staging and treatment.