Objective:Paclitaxel(PAC)is a chemotherapy drug and has an active role in the treatment of lung,breast,and ovarian cancers;however,its use causes increased levels of free radicals,leading to severe and irreversible or...Objective:Paclitaxel(PAC)is a chemotherapy drug and has an active role in the treatment of lung,breast,and ovarian cancers;however,its use causes increased levels of free radicals,leading to severe and irreversible organ damage.Thymus vulgaris is a species of flowering plant that contains various antioxidant chemical compounds.This study aimed to investigate the effect of T.vulgaris on PAC-induced oxidative damage in mice testis and sperm parameters.Methods:In this study,40 BALB/c mice were divided into five groups:normal control(NC);positive control(20 mg/kg of PAC);and three treatment groups(4.5,9,and 18 mg/kg doses of T.vulgaris extract+20 mg/kg PAC).Treatments were administered intraperitoneally daily for 50 days.Finally,the levels of serum luteinizing hormone(LH),follicle-stimulating hormone(FSH),and testosterone were measured using immunoassay method.Testicular stereological features and sperm parameters were also calculated.Antioxidant parameters were measured using nitrite oxide(NO)and perioxidation levels and ferric-reducing ability of plasma assay.The expressions of p53,caspase-3,Bax,and Bcl-2 were measured through real-time quantitative polymerase chain reaction.Results:T.vulgaris+PAC treatments at all doses significantly increased all parameters in NC and three treatment groups compared with the positive control group(20 mg/kg of PAC)(P<0.05),except the LH,FSH,and NO levels,which were decreased.Further,significantly downregulated levels of p53,caspase-3,and Bax genes and upregulated levels of Bcl-2 gene expression were noted in NC and three treatment groups in comparison with the positive control group(P<0.05).Conclusions:T.vulgaris administration attenuates the toxic effects of PAC on male reproductive parameters.展开更多
基金Research and Technology Deputy of Kermanshah University of Medical Sciences(97497)。
文摘Objective:Paclitaxel(PAC)is a chemotherapy drug and has an active role in the treatment of lung,breast,and ovarian cancers;however,its use causes increased levels of free radicals,leading to severe and irreversible organ damage.Thymus vulgaris is a species of flowering plant that contains various antioxidant chemical compounds.This study aimed to investigate the effect of T.vulgaris on PAC-induced oxidative damage in mice testis and sperm parameters.Methods:In this study,40 BALB/c mice were divided into five groups:normal control(NC);positive control(20 mg/kg of PAC);and three treatment groups(4.5,9,and 18 mg/kg doses of T.vulgaris extract+20 mg/kg PAC).Treatments were administered intraperitoneally daily for 50 days.Finally,the levels of serum luteinizing hormone(LH),follicle-stimulating hormone(FSH),and testosterone were measured using immunoassay method.Testicular stereological features and sperm parameters were also calculated.Antioxidant parameters were measured using nitrite oxide(NO)and perioxidation levels and ferric-reducing ability of plasma assay.The expressions of p53,caspase-3,Bax,and Bcl-2 were measured through real-time quantitative polymerase chain reaction.Results:T.vulgaris+PAC treatments at all doses significantly increased all parameters in NC and three treatment groups compared with the positive control group(20 mg/kg of PAC)(P<0.05),except the LH,FSH,and NO levels,which were decreased.Further,significantly downregulated levels of p53,caspase-3,and Bax genes and upregulated levels of Bcl-2 gene expression were noted in NC and three treatment groups in comparison with the positive control group(P<0.05).Conclusions:T.vulgaris administration attenuates the toxic effects of PAC on male reproductive parameters.
