Glinus lotoides ethanolic extract alleviates LPS-induced anxiety and depression-like behavior by modulating antioxidant and inflammatory biomarkers in rats

Objective:To explore the protective role of Glinus lotoides ethanolic extract in a depression model through modulating oxidant/antioxidant enzyme system and inflammatory status.Methods:Phytochemical constituents of Glinus lotoides ethanolic extract were evaluated qualitatively and quantitatively along with HPLC.Rats were divided into six groups.The normal control and the intoxicated groups received normal saline,and the standard group received imipramine,while the remaining groups received 100,300,and 500 mg/kg Glinus lotoides ethanolic extract.All groups received treatments for 14 d.Lipopolysaccharides(LPS)were then administered i.p.(0.83 mg/kg)to all groups except the normal control group.After 24 h,anxiety and depression-like behaviors were evaluated by performing behavioral analysis(open field,tail suspension,forced swim,sucrose preference test),and determining total oxidant status,total antioxidant capacity,catalase,and biochemical parameters[malondialdehyde,glutathione,superoxide dismutase,tumor necrosis factor(TNF)-alpha and interleukin(IL)-6].Results:Phytochemical studies confirmed the presence of phenols and flavonoids and HPLC analysis showed the presence of gallic acid,quercetin,chlorogenic,and caffeic acid.Total oxidant status was significantly decreased,while total antioxidant capacity was significantly increased in the Glinus lotoides ethanolic extract treated groups.Moreover,Glinus lotoides ethanolic extract diminished malondialdehyde,IL-6,and TNF-alpha levels,while increasing superoxide dismutase,catalase,and glutathione activities.Conclusions:Glinus lotoides ethanolic crude extract shows significant antidepressant activity by modulating oxidative and biochemical parameters that supports its folkloric use in traditional systems of medicine.

Salsola imbricata Forssk.ameliorates acetic acid-induced inflammatory bowel disease by modulating dysregulated antioxidant enzyme system and cytokine signaling pathways in mice

Objective:To explore the protective effect of the crude extract of Salsola imbricata against acetic acid-induced inflammatory bowel disease in mice and its mechanism of action.Methods:Ethanolic crude extract of Salsola imbricata was characterized by HPLC.Salsola imbricata extract at different doses was administered and ulcerative colitis was induced by 200μL,7.5%acetic acid and macroscopic parameters were evaluated to assess the homeostatic condition of intestinal mucosa along with hematological and biochemical assays.The levels of malondialdehyde,glutathione peroxidase 1,superoxide dismutase,and catalase were determined in colon tissues.Proinflammatory cytokines including interleukin(IL)-1β,IL-6,and tumor necrosis factor-α(TNF-α)were quantified by ELISA.The extent of tissue damage was assessed by histological analysis.Results:Phytochemical analysis confirmed the presence of phytochemicals including quercetin,gallic acid,syringic acid,benzoic acid and chlorogenic acid in the crude extract.The crude extract of Salsola imbricata(300 and 500 mg/kg)markedly decreased malondialdehyde and nitric oxide(P<0.01)and increased antioxidant activities of glutathione peroxidase 1(P<0.001)and superoxide dismutase(P<0.001).Moreover,it decreased the levels of IL-1β,IL-6 and TNF-αsignificantly(P<0.001)and reduced the damage to the colon mucosa,promoting tissue healing and regeneration.Conclusions:Salsola imbricata extract restores the colonic epithelial layers by maintaining mucosal homeostasis and cell integrity by modulating antioxidant defense system and inflammatory cytokine signaling in ulcerative colitis mice.