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乌拉尔图小麦叶绿体RNA编辑位点的预测与鉴定 被引量:3
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作者 葛玲巧 farhana kumbhar +4 位作者 赵贤 王萌 王莎 宋卫宁 聂小军 《分子植物育种》 CAS CSCD 北大核心 2017年第7期2479-2488,共10页
RNA编辑是指在RNA水平分子发生的碱基的改变或修饰,从而引起其所携带的遗传信息发生改变的过程。作为一种重要的转录后水平调控方式,RNA编辑在高等植物的生长发育、逆境响应以及物质生物合成等方面具有重要作用。为了解乌拉尔图小麦叶... RNA编辑是指在RNA水平分子发生的碱基的改变或修饰,从而引起其所携带的遗传信息发生改变的过程。作为一种重要的转录后水平调控方式,RNA编辑在高等植物的生长发育、逆境响应以及物质生物合成等方面具有重要作用。为了解乌拉尔图小麦叶绿体基因组RNA编辑的组成和特点,本研究利用生物信息学预测结合RT-PCR的方法对乌拉尔图小麦叶绿体的RNA编辑位点进行了预测与分析。生物信息学预测发现在76个蛋白质编码基因中,15个基因发生了RNA编辑现象,共计33个胞嘧啶(C)到尿嘧啶(U)的变化,其中ndhB基因发生的编辑位点最多;然后,随机选取5个基因进行了实验验证,初步验证了预测结果的准确性;进一步对这个5个基因编辑后其蛋白二级结构和跨膜结构域进行了预测,发现编辑后所有基因的二级结构均发生了改变,同时ndhB的跨膜结构域也发生了改变;最后,比较了乌拉尔图小麦与其他5个麦类作物叶绿体RNA编辑位点的异同,发现了叶绿体编辑位点在麦类作物中具有较强保守性,同时也发现了数个物种特异的编辑位点。本研究为进一步研究乌拉尔图小麦叶绿体RNA编辑的生物学功能提供帮助,为从RNA编辑视角揭示小麦的起源进化提供了重要参考。 展开更多
关键词 乌拉尔图小麦 叶绿体 RNA编辑 RT-PCR
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Use of Biochar as an Amendment for Remediation of Heavy Metal-Contaminated Soils:Prospects and Challenges 被引量:31
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作者 Altaf Hussain LAHORI GUO Zhanyu +8 位作者 ZHANG Zengqiang LI Ronghua Amanullah MAHAR Mukesh Kumar AWASTHI SHEN Feng Tanveer Ali SIAL farhana kumbhar WANG Ping JIANG Shuncheng 《Pedosphere》 SCIE CAS CSCD 2017年第6期991-1014,共24页
Biochar(BC), known as the new black gold, is a stable, novel carbonaceous by-product that is synthesized through pyrolysis of biological materials in the absence of O_2.Recently, an emerging interest in the applicatio... Biochar(BC), known as the new black gold, is a stable, novel carbonaceous by-product that is synthesized through pyrolysis of biological materials in the absence of O_2.Recently, an emerging interest in the application of BC as a robust soil amendment has given rise to a broad research area in science and technology.It is considered a promising remediation option for heavy metal(HM)-contaminated soils to reduce HM bioavailability to plants.Remediation efficacy of BC depends on the porosity, composition,pyrolysis temperature, feedstock, and residence time of pyrolysis.This review article aimed to present an overview of BC use in the immobilization of HMs, i.e., Cd, As, Pb, Zn, Ni, Cu, Mn, Cr, and Sb, in contaminated soils.The remaining uncertain factors, including the specific soil HM immobilization mechanisms, long-term beneficial effects, and potential environmental risks associated with BC application are analyzed.Future research must be conducted to ensure that the management of environmental pollution is in accord with ecological sustainability and adaptation of the black gold biotechnology on a commercial basis for immobilization of HMs in contaminated soils. 展开更多
关键词 additives environmental pollution FEEDSTOCK IMMOBILIZATION pyrolysis temperature remediation efficacy
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