Centella asiatica (L.), frequently known as Thankuni, is an important ethnobotanical plant in Bangladesh. This study was conducted to evaluate the morphological characteristics, cultural factors and molecular identifi...Centella asiatica (L.), frequently known as Thankuni, is an important ethnobotanical plant in Bangladesh. This study was conducted to evaluate the morphological characteristics, cultural factors and molecular identification of the causal agent of Alternaria leaf blight disease of C. asiatica. The potato dextrose agar (PDA) medium recorded the maximum mycelial growth (69 mm), followed by the yeast extract agar (YEA) medium, while the honey peptone agar (HPA) medium recorded the lowest growth (27 mm). The optimal pH and temperature for mycelial growth of Alternaria alternata were 6 and 30°C, respectively. Internal transcribed spacer (ITS) region of Alternaria alternata PCR products measured 558 bp and blast search showed 99% sequence similarity with Alternaria alternata species complex. To the best of our knowledge, Alternaria leaf blight disease caused by Alternaria alternata is the first record in Bangladesh.展开更多
Due to poverty and ignorance more than half of the pregnant women in developing country are suffering from iron deficiency anaemia (IDA). Different gastrointestinal side effects cause low compliance to iron supplement...Due to poverty and ignorance more than half of the pregnant women in developing country are suffering from iron deficiency anaemia (IDA). Different gastrointestinal side effects cause low compliance to iron supplementation. Amloki (<em>Emblica officinalis</em>) is very popular among pregnant women for its taste and traditional belief of well-being. The aim of this study is to determine the effects of amloki on RBC, WBC and Platelet count. Prospective comparative study was carried out on patients of Outpatient Department of Obstetrics and Gynecology, Dhaka Medical College Hospital from July 2016 to June 2017. A total of 43 pregnant women between the 13th to the 20th weeks of gestation with IDA were selected aged 18 - 36 years in this study. Anemic pregnant women supplemented with oral iron and amloki were considered as study group (A) and control group (B) were with only iron supplementation for 45 days. Total count of RBC, WBC and platelet were done in the Department of Hematology & BMT Unit, Dhaka Medical College Hospital, Dhaka. For statistical analysis, Paired Student’s “t” test and Unpaired Student’s “t” test were considered using SPSS 22.0 version. A significant increase (p < 0.05) of blood RBC count was observed after intervention of iron in both groups. There was also significant increase in RBC count in iron + amloki supplemented group than that of only iron supplemented group. In this study, there was also increase of WBC count in iron + amloki supplemented group and decrease of platelet count in both groups, but these changes were not statistically significant. It can be concluded that oral amloki supplementation causes increased blood RBC count in IDA with pregnancy.展开更多
文摘Centella asiatica (L.), frequently known as Thankuni, is an important ethnobotanical plant in Bangladesh. This study was conducted to evaluate the morphological characteristics, cultural factors and molecular identification of the causal agent of Alternaria leaf blight disease of C. asiatica. The potato dextrose agar (PDA) medium recorded the maximum mycelial growth (69 mm), followed by the yeast extract agar (YEA) medium, while the honey peptone agar (HPA) medium recorded the lowest growth (27 mm). The optimal pH and temperature for mycelial growth of Alternaria alternata were 6 and 30°C, respectively. Internal transcribed spacer (ITS) region of Alternaria alternata PCR products measured 558 bp and blast search showed 99% sequence similarity with Alternaria alternata species complex. To the best of our knowledge, Alternaria leaf blight disease caused by Alternaria alternata is the first record in Bangladesh.
文摘Due to poverty and ignorance more than half of the pregnant women in developing country are suffering from iron deficiency anaemia (IDA). Different gastrointestinal side effects cause low compliance to iron supplementation. Amloki (<em>Emblica officinalis</em>) is very popular among pregnant women for its taste and traditional belief of well-being. The aim of this study is to determine the effects of amloki on RBC, WBC and Platelet count. Prospective comparative study was carried out on patients of Outpatient Department of Obstetrics and Gynecology, Dhaka Medical College Hospital from July 2016 to June 2017. A total of 43 pregnant women between the 13th to the 20th weeks of gestation with IDA were selected aged 18 - 36 years in this study. Anemic pregnant women supplemented with oral iron and amloki were considered as study group (A) and control group (B) were with only iron supplementation for 45 days. Total count of RBC, WBC and platelet were done in the Department of Hematology & BMT Unit, Dhaka Medical College Hospital, Dhaka. For statistical analysis, Paired Student’s “t” test and Unpaired Student’s “t” test were considered using SPSS 22.0 version. A significant increase (p < 0.05) of blood RBC count was observed after intervention of iron in both groups. There was also significant increase in RBC count in iron + amloki supplemented group than that of only iron supplemented group. In this study, there was also increase of WBC count in iron + amloki supplemented group and decrease of platelet count in both groups, but these changes were not statistically significant. It can be concluded that oral amloki supplementation causes increased blood RBC count in IDA with pregnancy.
