Family Cucurbitaceae is primarily found in the warmer regions of the world. It is the major family for economically important species, particularly edible fruits. In Pakistan cucurbits occupies an area of 28,600 ha wi...Family Cucurbitaceae is primarily found in the warmer regions of the world. It is the major family for economically important species, particularly edible fruits. In Pakistan cucurbits occupies an area of 28,600 ha with a very low production in Khyber Pukhtunkhwa due to many biotic and abiotic stresses. The reason is also the lack of growers’ awareness about the diseases and the cultural practices adopted to provide favorable environment for development of epidemics. Viral diseases such as Cucumber mosaic virus (CMV) cause losses as high as 100%. Various control strategies are being used to control CMV. The aim of the current study was to screen out different verities and to find the most resistant one against CMV. CMV isolate was collected from farmer’s field at the site of TaruJaba during a survey of cucurbit crops. The identity of the virus was confirmed through DAS-ELISA using diagnostic kit (ADGEN, UK). Seventeen cucumber germplasm seeds were sown in earthen pots in which fourteen were germinated and exhibited characteristics symptoms of the virus while none of them showed resistance against CMV. Symptoms’ expression was delayed in summer green and local green till 12 days post inoculation. While in khyber, Diamond, VEGAF1 and Yousaf, symptoms started to appear soon after inoculation categorizing them as highly susceptible. No resistance is found in available commercial germplasm, so more germplasm from different area of Pakistan should be tested for resistance against CMV. If no resistance is found locally imported, germplasm can be evaluated for a source of resistance against the prevalent isolates of CMV.展开更多
文摘Family Cucurbitaceae is primarily found in the warmer regions of the world. It is the major family for economically important species, particularly edible fruits. In Pakistan cucurbits occupies an area of 28,600 ha with a very low production in Khyber Pukhtunkhwa due to many biotic and abiotic stresses. The reason is also the lack of growers’ awareness about the diseases and the cultural practices adopted to provide favorable environment for development of epidemics. Viral diseases such as Cucumber mosaic virus (CMV) cause losses as high as 100%. Various control strategies are being used to control CMV. The aim of the current study was to screen out different verities and to find the most resistant one against CMV. CMV isolate was collected from farmer’s field at the site of TaruJaba during a survey of cucurbit crops. The identity of the virus was confirmed through DAS-ELISA using diagnostic kit (ADGEN, UK). Seventeen cucumber germplasm seeds were sown in earthen pots in which fourteen were germinated and exhibited characteristics symptoms of the virus while none of them showed resistance against CMV. Symptoms’ expression was delayed in summer green and local green till 12 days post inoculation. While in khyber, Diamond, VEGAF1 and Yousaf, symptoms started to appear soon after inoculation categorizing them as highly susceptible. No resistance is found in available commercial germplasm, so more germplasm from different area of Pakistan should be tested for resistance against CMV. If no resistance is found locally imported, germplasm can be evaluated for a source of resistance against the prevalent isolates of CMV.
