Five novel heterodimers of cytochalasan and ergosterol,ergochaeglobosins A—E,were isolated from an endophytic fungus Chaetomium globosum P2-2-2.Ergochaeglobosin A(1) possesses an unprecedented 5/6/13/6/5/6/6/6 fused ...Five novel heterodimers of cytochalasan and ergosterol,ergochaeglobosins A—E,were isolated from an endophytic fungus Chaetomium globosum P2-2-2.Ergochaeglobosin A(1) possesses an unprecedented 5/6/13/6/5/6/6/6 fused octacyclic ring system,and ergochaeglobosins B(2) and C(3) form a unique 5/6/13/6/6/6/6/5 fused octacyclic ring system,whereas ergochaeglobosins D(4) and F(5) are the first cytochalasan heterodimers formed via a N-1′/C-3′′single bond.Their structures with absolute configurations were established by extensive spectroscopic analyses,single-crystal X-ray diffraction,and ECD calculations.The plausible biogenetic pathway of 1—5 was postulated.Compound 1 exhibited inhibitory effects against ConA-induced T cells and LPS-induced B cells proliferation with IC50 values of 19.2 and 14.1μmol/L,respectively.Co-treatment of 3(20μmol/L)with TRAIL(150 ng/mL)could significantly decrease the cell viability of A549 by 24.9%comparing with monotherapy of 3(20μmol/L).展开更多
Natural products(NPs)are a valuable source in the food,pharmaceutical,agricultural,environmental,and many other industrial sectors.Their beneficial properties along with their potential toxicities make the detection,d...Natural products(NPs)are a valuable source in the food,pharmaceutical,agricultural,environmental,and many other industrial sectors.Their beneficial properties along with their potential toxicities make the detection,determination or quantification of NPs essential for their application.The advanced instrumental methods require time-consuming sample preparation and analysis.In contrast,biosensors allow rapid detection of NPs,especially in complex media,and are the preferred choice of detection when speed and high throughput are intended.Here,we review diverse biosensors reported for the detection of NPs.The emerging approaches for improving the efficiency of biosensors,such as microfluidics,nanotechnology,and magnetic beads,are also discussed.The simultaneous use of two detection techniques is suggested as a robust strategy for precise detection of a specific NP with structural complexity in complicated matrices.The parallel detection of a variety of NPs structures or biological activities in a mixture of extract in a single detection phase is among the anticipated future advancements in this field which can be achieved using multisystem biosensors applying multiple flow cells,sensing elements,and detection mechanisms on miniaturized folded chips.展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.22077041 and 31770380)。
文摘Five novel heterodimers of cytochalasan and ergosterol,ergochaeglobosins A—E,were isolated from an endophytic fungus Chaetomium globosum P2-2-2.Ergochaeglobosin A(1) possesses an unprecedented 5/6/13/6/5/6/6/6 fused octacyclic ring system,and ergochaeglobosins B(2) and C(3) form a unique 5/6/13/6/6/6/6/5 fused octacyclic ring system,whereas ergochaeglobosins D(4) and F(5) are the first cytochalasan heterodimers formed via a N-1′/C-3′′single bond.Their structures with absolute configurations were established by extensive spectroscopic analyses,single-crystal X-ray diffraction,and ECD calculations.The plausible biogenetic pathway of 1—5 was postulated.Compound 1 exhibited inhibitory effects against ConA-induced T cells and LPS-induced B cells proliferation with IC50 values of 19.2 and 14.1μmol/L,respectively.Co-treatment of 3(20μmol/L)with TRAIL(150 ng/mL)could significantly decrease the cell viability of A549 by 24.9%comparing with monotherapy of 3(20μmol/L).
文摘Natural products(NPs)are a valuable source in the food,pharmaceutical,agricultural,environmental,and many other industrial sectors.Their beneficial properties along with their potential toxicities make the detection,determination or quantification of NPs essential for their application.The advanced instrumental methods require time-consuming sample preparation and analysis.In contrast,biosensors allow rapid detection of NPs,especially in complex media,and are the preferred choice of detection when speed and high throughput are intended.Here,we review diverse biosensors reported for the detection of NPs.The emerging approaches for improving the efficiency of biosensors,such as microfluidics,nanotechnology,and magnetic beads,are also discussed.The simultaneous use of two detection techniques is suggested as a robust strategy for precise detection of a specific NP with structural complexity in complicated matrices.The parallel detection of a variety of NPs structures or biological activities in a mixture of extract in a single detection phase is among the anticipated future advancements in this field which can be achieved using multisystem biosensors applying multiple flow cells,sensing elements,and detection mechanisms on miniaturized folded chips.
