BACKGROUND Congenital agenesis of the gallbladder(CAGB)is a rare condition often misdiagnosed as cholecystolithiasis,leading to unnecessary surgeries.Accurate diagnosis and surgical exploration are crucial in patients...BACKGROUND Congenital agenesis of the gallbladder(CAGB)is a rare condition often misdiagnosed as cholecystolithiasis,leading to unnecessary surgeries.Accurate diagnosis and surgical exploration are crucial in patients with suspected CAGB or atypical gallbladder stone symptoms.Preoperative imaging,such as magnetic resonance cholangiopancreatography(MRCP),plays a vital role in confirming the diagnosis.Careful intraoperative dissection is necessary to avoid iatrogenic injuries and misdiagnosis.Multidisciplinary consultations and collaboration,along with the use of various diagnostic methods,can minimize associated risks.CASE SUMMARY We present the case of a 34-year-old female with suspected gallbladder stones,ultimately diagnosed with CAGB through surgical exploration.The patient underwent laparoscopic examination followed by open exploratory surgery,which confirmed absence of the gallbladder.Subsequent imaging studies supported the diagnosis.The patient received appropriate postoperative care and experienced a successful recovery.CONCLUSION This case highlights the rarity of CAGB and the importance of considering this condition in the differential diagnosis of patients with gallbladder stone symptoms.Accurate diagnosis using preoperative imaging,such as MRCP,is crucial to prevent unnecessary surgeries.Surgeons should exercise caution and conduct meticulous dissection during surgery to avoid iatrogenic injuries and ensure accurate diagnosis.Multidisciplinary collaboration and utilization of various diagnostic methods are essential to minimize the risk of misdiagnosis.Selection of the optimal treatment strategy should prioritize minimizing trauma and maintaining open communication with the patient and their family members.展开更多
The research progress of a novel traffic solution,a submerged floating tunnel(SFT),is reviewed in terms of a study approach and loading scenario.Among existing publications,the buoyancy-weight ratio(BWR) is usually pr...The research progress of a novel traffic solution,a submerged floating tunnel(SFT),is reviewed in terms of a study approach and loading scenario.Among existing publications,the buoyancy-weight ratio(BWR) is usually predefined.However,BWR is a critical structural parameter that tremendously affects the dynamic behaviour of not only the tunnel tube itself but also the cable system.In the context of a SFT prototype(SFTP) project in Qiandao Lake(Zhejiang Province,China),the importance of BWR is illustrated by finite element analysis and subsequently,an optimized BWR is proposed within a reasonable range in the present study.In the numerical model,structural damping is identified to be of importance.Rayleigh damping and the corresponding Rayleigh coefficients are attained through a sensitivity study,which shows that the adopted damping ratios are fairly suitable for SFTP.Lastly,the human sense of security is considered by quantifying the comfort index,which helps further optimize BWR in the SFTP structural parameter design.展开更多
Background context:Metastatic spinal cord compression(MSCC)seriously affects the survival rate.Objective:The therapeutic effects of two treatment strategies for MSCC:percutaneous vertebroplasty(PVP)combined with radio...Background context:Metastatic spinal cord compression(MSCC)seriously affects the survival rate.Objective:The therapeutic effects of two treatment strategies for MSCC:percutaneous vertebroplasty(PVP)combined with radiofrequency ablation(RFA)and PVP combined with^(125)I particle implantation,were compared.Study design:Retrospective study.Patient sample:40 patients with MSCC were divided into two groups:19 cases in the RFA group and 21 cases in the^(125)I group.Method:All patients were accessed to determine the differences in pain,which was evaluated using the visual analog scale(VAS)at 1 week,1 month,and 3 months after the operation,and spinal stenosis rates(SSRs),which were measured at 1 and 3 months after the operation,between the two groups.Results:The VAS scores and SSRs at baseline were comparable between the RFA group and the^(125)I group(7.19±2.07 vs 7.42±1.95,37.7%±11.2%vs 41.1%±11.4%).The VAS scores and SSRs at 1 month and 3 months after the operation were significantly reduced in both groups,compared with those at baseline.The VAS scores and SSRs in the^(125)I group were lower than those in the RFA group at 3 months after the operation(1.09±0.97 vs 1.75±1.06 p=0.048 and 12.3%±6.4%vs 18.1%±10.1%p=0.034),while the VAS scores at1 week after the operation in the RFA group were lower than those in the^(125)I group(4.39±1.34 vs 5.05±1.82 p=0.049).Conclusion:PVP combined with RFA has a slight advantage in relieving pain in the short term,while PVP combined with^(125)I particle implantation may have a better effect in the relieving pain and decreasing the SSRs at 3 months after the operation.展开更多
The current assembled maize genomes cannot represent the broad genetic diversity of maize germplasms.Acquiring more genome sequences is critical for constructing a pan-genome and elucidating the linkage between genoty...The current assembled maize genomes cannot represent the broad genetic diversity of maize germplasms.Acquiring more genome sequences is critical for constructing a pan-genome and elucidating the linkage between genotype and phenotype in maize.Here we describe the genome sequence and annotation of A188,a maize inbred line with high phenotypic variation relative to other lines,acquired by single-molecule sequencing and optical genome mapping.We assembled a 2210-Mb genome with a scaffold N50 size of 11.61 million bases(Mb),compared to 9.73 Mb for B73 and 10.2 Mb for Mo17.Based on the B73_Ref Gen_V4 genome,295 scaffolds(2084.35 Mb,94.30%of the final genome assembly)were anchored and oriented on ten chromosomes.Comparative analysis revealed that~30%of the predicted A188 genes showed large structural divergence from B73,Mo17,and W22 genomes,which causes high protein divergence and may lead to phenotypic variation among the four inbred lines.As a line with high embryonic callus(EC)induction capacity,A188 provides a convenient tool for elucidating the molecular mechanism underlying the formation of EC in maize.Combining our new A188 genome with previously reported QTL and RNA sequencing data revealed eight genes with large structural variation and two differentially expressed genes playing potential roles in maize EC induction.展开更多
The WW domain-containing oxidoreductase(WWOX) is a tumor suppressor in a variety of cancers, including breast cancer. Reduced WWOX expression is associated with the basal-like subtype and a relatively poor disease-fre...The WW domain-containing oxidoreductase(WWOX) is a tumor suppressor in a variety of cancers, including breast cancer. Reduced WWOX expression is associated with the basal-like subtype and a relatively poor disease-free survival rate among breast cancer patients. Though several WWOX partners have been identified, the functional mechanisms of WWOX's role in cancers have not been fully addressed to date. In the current study, we found WWOX suppresses expression of KLF5—an oncogenic transcription factor—at protein level, and suppresses cancer cell proliferation in both bladder and breast cancer cell lines. Furthermore, we demonstrated that WWOX physically interacts with KLF5 via the former's WW domains and the latter's PY motifs. Interestingly, we found the expression of WWOX negatively correlates with KLF5 expression in a panel of breast cancer cell lines. Taken together, we conjecture that WWOX may suppress cancer cell proliferation partially by reducing the expression of KLF5.展开更多
The effects of tension on vortex-induced vibration(ⅥⅤ) responses for a tension-dominated long cylinder with an aspect ratio of 550 in uniform flows are experimentally investigated in this paper. The results show tha...The effects of tension on vortex-induced vibration(ⅥⅤ) responses for a tension-dominated long cylinder with an aspect ratio of 550 in uniform flows are experimentally investigated in this paper. The results show that elevated tension suppresses fluctuations of maximum displacement with respect to flow velocity and makes chaotic ⅥⅤ more likely to appear. With respect to periodic ⅥⅤ, if elevated tension is applied, the dominant vibration frequency in the in-line(IL) direction will switch from a fundamental vibration frequency to twice the value of the fundamental vibration frequency, which results in a ratio of the dominant vibration frequency in the IL direction to that in the cross-flow direction of 2.0. The suppression of the elevated tension in the fluctuation of the maximum displacement causes the axial tension to become an active control parameter for the ⅥⅤ maximum displacement of a tension-dominated long riser or tether of an engineering structure in deep oceans. However,the axial tension must be optimized before being used since the high dominant vibration frequency due to the elevated tension may unfavorably affect the fatigue life of the riser or tether.展开更多
Adenosine triphosphate(ATP)generation of aquatic organisms is often subject to nanoparticles(NPs)stress,involving extensive reprogramming of gene expression and changes in enzyme activity accompanied by metabolic dist...Adenosine triphosphate(ATP)generation of aquatic organisms is often subject to nanoparticles(NPs)stress,involving extensive reprogramming of gene expression and changes in enzyme activity accompanied by metabolic disturbances.However,little is known about the mechanism of energy supply by ATP to regulate the metabolism of aquatic organisms under NPs stress.Here,we selected extensively existing silver nanoparticles(AgNPs)to investigate their implications on ATP generation and relevant metabolic pathways in alga(Chlorella vulgaris).Results showed that ATP content significantly decreased by 94.2%of the control(without AgNPs)in the algal cells at 0.20 mg/L AgNPs,which was mainly attributed to the reduction of chloroplast ATPase activity(81.4%)and the downregulation of ATPase-coding genes atpB and atpH(74.5%-82.8%)in chloroplast.Molecular dynamics simulations demonstrated that AgNPs competed with the binding sites of substrates adenosine diphosphate and inorganic phosphate by forming a stable complex with ATPase subunit beta,potentially resulting in the reduced binding efficiency of substrates.Furthermore,metabolomics analysis proved that the ATP content positively correlated with the content of most differential metabolites such as D-talose,myo-inositol,and L-allothreonine.AgNPs remarkably inhibited ATPinvolving metabolic pathways,including inositol phosphate metabolism,phosphatidylinositol signaling system,glycerophospholipid metabolism,aminoacyl-tRNA biosynthesis,and glutathione metabolism.These results could provide a deep understanding of energy supply in regulating metabolic disturbances under NPs stress.展开更多
Purpose:This paper proposes a method of detecting emerging trends in research topics from a more micro perspective.Design/methodology/approach:Through co-word clustering to identify research topics and analyzing posit...Purpose:This paper proposes a method of detecting emerging trends in research topics from a more micro perspective.Design/methodology/approach:Through co-word clustering to identify research topics and analyzing position changes of topic words in the keywords life-cycle diagram during different time periods,we detected emerging trends in research topics from a more micro perspective.I'he method was applied to the field of nanotechnology to verify its effectiveness and practicability.Findings:The results show that this method can be used to detect emerging trends in research topics from a more micro perspective,as it divides keywords into five categories:Potential keywords,emerging keywords,hot keywords,stable keywords and recession keywords,through which more details of topic changes can be found.Research limitations:We used keywords provided by authors and database indexers for keywords extraction.But this approach may lead to the problem of "indexer effect".The method may have a limited effect when applied to a disciplinary domain such as mathematics,which evolves slowly.Practical implications:This study provides information analysts with insights into the way to better understand specialty areas of a discipline domain and formulate research policies and strategic plans.Originality/value:This study contributes to the current literature by proposing a new method,which can detect emerging trends in research topics from a more micro perspective.展开更多
Metastasis is the leading cause of cancer-related death.The interactions between circulating tumor cells and endothelial adhesion molecules in distant organs is a key step during extravasation in hematogenous metastas...Metastasis is the leading cause of cancer-related death.The interactions between circulating tumor cells and endothelial adhesion molecules in distant organs is a key step during extravasation in hematogenous metastasis.Surgery is a common intervention for most primary solid tumors.However,surgical trauma-related systemic inflammation facilitates distant tumor metastasis by increasing the spread and adhesion of tumor cells to vascular endothelial cells(ECs).Currently,there are no effective interventions to prevent distant metastasis.Here,we show that HECTD3 deficiency in ECs significantly reduces tumor metastasis in multiple mouse models.HECTD3 depletion downregulates expression of adhesion molecules,such as VCAM-1,ICAM-1 and E-selectin,in mouse primary ECs and HUVECs stimulated by inflammatory factors and inhibits adhesion of tumor cells to ECs both in vitro and in vivo.We demonstrate that HECTD3 promotes stabilization,nuclear localization and kinase activity of IKKa by ubiquitinating IKKa with K27-and K63-linked polyubiquitin chains at K296,increasing phosphorylation of histone H3 to promote NF-kB target gene transcription.Knockout of HECTD3 in endothelium significantly inhibits tumor cells lung colonization,while conditional knockin promotes that.IKKa kinase inhibitors prevented LPS-induced pulmonary metastasis.These findings reveal the promotional role of the HECTD3-IKKa axis in tumor hematogenous metastasis and providea potential strategy for tumormetastasis prevention.展开更多
This study investigated the transformations of heavy metals(HMs) and polycyclic aromatic hydrocarbons(PAHs) in rhizosphere soils and adaptive responses of rhizobacterial community under the real field conditions durin...This study investigated the transformations of heavy metals(HMs) and polycyclic aromatic hydrocarbons(PAHs) in rhizosphere soils and adaptive responses of rhizobacterial community under the real field conditions during four growth stages(e.g., greening, tillering, heading, and maturity) of early rice(Zhongjiazao 17) and late rice(Zhongyou 9918) in Jiangshe village(JSV) and Yangji village(YJV). Results showed that rhizosphere soils of YJV were mildly polluted by Cd and PAHs compared to that of JSV. The relative abundance of bioavailable Cd(bio-Cd) and bioavailable As(bio-As) in rhizosphere soil increased before the heading stage but decreased at the subsequent growth stage, but the content of ΣPAHs in rhizosphere soil decreased gradually during whole growth period. The dominant rhizobacteria genera at YJV(e.g., Bacillus, Massilia, Sphingomonas, and Geobacter) increased at an abundance level from the tillering to heading stage. Rhizobacteria interacted with the above co-pollutant more intensely at the tillering and heading stage, where genes involved in HM-resistance and PAH-degradation appeared to have a significant enhancement. The contents of bioCd and bio-As in rhizosphere soil of early rice were higher than that of late rice at each growth stage, especially at the heading stage. Bio-Cd, ΣPAHs, and organic matter were key factors influencing the community structure of rhizobacteria. Results of this study provide valuable insights about the interactions between HM-PAH co-pollutant and rhizobacterial community under real field conditions and thus develop in-situ rhizosphere remediation techniques for contaminated paddy fields.展开更多
We report that green algae in lakes and rivers can serve as precursors of halobenzoquinone(HBQ) disinfection byproducts(DBPs) produced during chlorination. Chlorination of a common green alga, Chlorella vulgaris, prod...We report that green algae in lakes and rivers can serve as precursors of halobenzoquinone(HBQ) disinfection byproducts(DBPs) produced during chlorination. Chlorination of a common green alga, Chlorella vulgaris, produced 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ), the most prevalent HBQ DBP in disinfected water. Under varying p H conditions(p H 6.0–9.0), 2,6-DCBQ formation ranged from 0.3 to 2.1 μg/mg C with maximum formation at p H 8.0. To evaluate the contribution of organic components of C. vulgaris to 2,6-DCBQ formation, we separated the organics into two fractions, the protein-rich fraction of intracellular organic matter(IOM) and the polysaccharide-laden fraction of extracellular organic matter(EOM). Chlorination of IOM and EOM produced 1.4 μg/mg C and 0.7 μg/mg C of 2,6-DCBQ, respectively. The IOM generated a two-fold higher 2,6-DCBQ formation potential than the EOM fraction, suggesting that proteins are potent 2,6-DCBQ precursors. This was confirmed by the chlorination of proteins extracted from C. vulgaris: the amount of 2,6-DCBQ produced is linearly correlated with the concentration of total algal protein(R2= 0.98). These results support that proteins are the primary precursors of 2,6-DCBQ in algae, and control of green algal bloom outbreaks in source waters is important for management of HBQ DBPs.展开更多
Ultrafine chitosan particles were prepared by reverse microemulsion consisting of water, Triton X-100, octanol and cyclohexane. Two methods of preparing ultrafine chitosan particles were adopted and compared using TEM...Ultrafine chitosan particles were prepared by reverse microemulsion consisting of water, Triton X-100, octanol and cyclohexane. Two methods of preparing ultrafine chitosan particles were adopted and compared using TEM and IR, and possible mechanisms for the formation of ultrafine chitosan particles were proposed. Experimental results show that the method which combined ionic gelation and cross-linking gave uniformly sized chitosan nanoparticles with an average diameter of 92 nm, while the cross-linking without ionic gelation produced spindly chitosan particles with an average length of 943 nm and width of 188 nm.展开更多
Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer cells have a higher dependency on molecular chaperones to maintain pro...Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer cells have a higher dependency on molecular chaperones to maintain proteostasis. The chaperonin T-complex protein ring complex(TRiC) contains eight paralogous subunits(CCT1-8), and assists the folding of as many as 10% of cytosolic proteome.TRiC is essential for the progression of some cancers, but the roles of TRiC subunits in osteosarcoma remain to be explored. Here, we show that CCT4/TRiC is significantly correlated in human osteosarcoma,and plays a critical role in osteosarcoma cell survival. We identify a compound anticarin-β that can specifically bind to and inhibit CCT4. Anticarin-β shows higher selectivity in cancer cells than in normal cells. Mechanistically, anticarin-β potently impedes CCT4-mediated STAT3 maturation. Anticarin-β displays remarkable antitumor efficacy in orthotopic and patient-derived xenograft models of osteosarcoma.Collectively, our data uncover a key role of CCT4 in osteosarcoma, and propose a promising treatment strategy for osteosarcoma by disrupting CCT4 and proteostasis.展开更多
Commercial vehicle industry worldwide is facing challenges from environmental pressures,stringent limits of CO_(2) emission,governmental regulations as well as ever-increased customer demands.This paper analyzes the a...Commercial vehicle industry worldwide is facing challenges from environmental pressures,stringent limits of CO_(2) emission,governmental regulations as well as ever-increased customer demands.This paper analyzes the above-mentioned challenges,especially in China,including the potential improvement to increase the brake thermal efficiency(BTE),with five levels of BTE proposed,ranging from current 45 to 60%in future,corresponding to China fuel consumption regulation(CFCR)in different phases.The authors also proposed the technology roadmaps to meet the upcoming CFCR3 and CFCR4;finally,the authors draw the conclusions to conform with ever-stringent regulation in China.展开更多
To systematically assess the effectiveness and safety of using acupuncture with traditional Chinese medicine for functional constipation so as to provide the evidence-based data for clinical practice.Wanfang databases...To systematically assess the effectiveness and safety of using acupuncture with traditional Chinese medicine for functional constipation so as to provide the evidence-based data for clinical practice.Wanfang databases,CBM,VIP,CNKI,PubMed,Cochrane Library and EMbase were searched for trials based on randomization and control for use of acupuncture in combination with CHM for functional constipation published from the inception of the database to Dec.2019.We used the risk of bias of Cochrane Collaboration for literature quality assessment,extracted trial data in duplicate,and then assessed the risk of bias.The data were discussed by adopting Review Manager 5.3 program.A total of 22 RCTs with 2574 cases were retrieved.Meta-analysis showed that acupuncture with CHM could improve the clinical effectiveness(OR6.10,95%CI 4.72–7.87,P<0.00001);heterogeneity was not identified(Chi^(2)=32.84,P=0.43,I^(2)=2%),and the difference was statistically significant.Second-level outcomes(e.g.,defecation interval time(P<0.00001),Bristol Stool Scale(P=0.002),defecation difficulty(P=0.01)and accompanying symptoms(P<0.00001))in the treatment groups were significantly different after the acupuncture with CHM compared with control groups,whereas defecation symptoms were not significantly different(P=0.06).The use of acupuncture with CHM can significantly improve the clinical efficacy of functional constipation,ameliorate Bristol Stool Scale,defecation difficulty,defecation symptoms,and accompanying symptoms,as well as shorten the defecation interval time,with better safety.展开更多
Aggregation-induced emission(AIE)is a vital photophysical phenomenon that the luminogens in the concentrated or aggregated cases will engender the dramatically boosted emission in comparison with the dispersive states...Aggregation-induced emission(AIE)is a vital photophysical phenomenon that the luminogens in the concentrated or aggregated cases will engender the dramatically boosted emission in comparison with the dispersive states.Given this extraordinary emitting capacity exactly resolves the aggregation-caused quenching(ACQ)situations residing in the traditional luminophores,the booming AIE luminogens have drawn tremendous interest owing to their advanced performances and colossal potential applications in various areas.Further exploitations of AIE molecules also drive the research interests in the midst of these AIE materials toward the nonlinear optical(NLO)regime.The combination of AIE and NLO effects have nurtured some unforeseen properties of AIE materials and extended their application spheres.Therefore,some NLO-active AIE materials have been wielded in many crucial applications,for example,optical limiting,laser,bioimaging,and photodynamic therapy.Meanwhile,the impacts of aggregate on the NLO effect also deserve deep considerations and pursuits,and the modifications of aggregates promise an easy,efficient,and prompt avenue to tune the NLO properties of materials.The recent achievements and progress in the NLO properties of AIE materials have been summarized in this review.The second-order and third-order NLOs of the AIE materials have been introduced and their correlative applications have been discussed.展开更多
文摘BACKGROUND Congenital agenesis of the gallbladder(CAGB)is a rare condition often misdiagnosed as cholecystolithiasis,leading to unnecessary surgeries.Accurate diagnosis and surgical exploration are crucial in patients with suspected CAGB or atypical gallbladder stone symptoms.Preoperative imaging,such as magnetic resonance cholangiopancreatography(MRCP),plays a vital role in confirming the diagnosis.Careful intraoperative dissection is necessary to avoid iatrogenic injuries and misdiagnosis.Multidisciplinary consultations and collaboration,along with the use of various diagnostic methods,can minimize associated risks.CASE SUMMARY We present the case of a 34-year-old female with suspected gallbladder stones,ultimately diagnosed with CAGB through surgical exploration.The patient underwent laparoscopic examination followed by open exploratory surgery,which confirmed absence of the gallbladder.Subsequent imaging studies supported the diagnosis.The patient received appropriate postoperative care and experienced a successful recovery.CONCLUSION This case highlights the rarity of CAGB and the importance of considering this condition in the differential diagnosis of patients with gallbladder stone symptoms.Accurate diagnosis using preoperative imaging,such as MRCP,is crucial to prevent unnecessary surgeries.Surgeons should exercise caution and conduct meticulous dissection during surgery to avoid iatrogenic injuries and ensure accurate diagnosis.Multidisciplinary collaboration and utilization of various diagnostic methods are essential to minimize the risk of misdiagnosis.Selection of the optimal treatment strategy should prioritize minimizing trauma and maintaining open communication with the patient and their family members.
文摘The research progress of a novel traffic solution,a submerged floating tunnel(SFT),is reviewed in terms of a study approach and loading scenario.Among existing publications,the buoyancy-weight ratio(BWR) is usually predefined.However,BWR is a critical structural parameter that tremendously affects the dynamic behaviour of not only the tunnel tube itself but also the cable system.In the context of a SFT prototype(SFTP) project in Qiandao Lake(Zhejiang Province,China),the importance of BWR is illustrated by finite element analysis and subsequently,an optimized BWR is proposed within a reasonable range in the present study.In the numerical model,structural damping is identified to be of importance.Rayleigh damping and the corresponding Rayleigh coefficients are attained through a sensitivity study,which shows that the adopted damping ratios are fairly suitable for SFTP.Lastly,the human sense of security is considered by quantifying the comfort index,which helps further optimize BWR in the SFTP structural parameter design.
文摘Background context:Metastatic spinal cord compression(MSCC)seriously affects the survival rate.Objective:The therapeutic effects of two treatment strategies for MSCC:percutaneous vertebroplasty(PVP)combined with radiofrequency ablation(RFA)and PVP combined with^(125)I particle implantation,were compared.Study design:Retrospective study.Patient sample:40 patients with MSCC were divided into two groups:19 cases in the RFA group and 21 cases in the^(125)I group.Method:All patients were accessed to determine the differences in pain,which was evaluated using the visual analog scale(VAS)at 1 week,1 month,and 3 months after the operation,and spinal stenosis rates(SSRs),which were measured at 1 and 3 months after the operation,between the two groups.Results:The VAS scores and SSRs at baseline were comparable between the RFA group and the^(125)I group(7.19±2.07 vs 7.42±1.95,37.7%±11.2%vs 41.1%±11.4%).The VAS scores and SSRs at 1 month and 3 months after the operation were significantly reduced in both groups,compared with those at baseline.The VAS scores and SSRs in the^(125)I group were lower than those in the RFA group at 3 months after the operation(1.09±0.97 vs 1.75±1.06 p=0.048 and 12.3%±6.4%vs 18.1%±10.1%p=0.034),while the VAS scores at1 week after the operation in the RFA group were lower than those in the^(125)I group(4.39±1.34 vs 5.05±1.82 p=0.049).Conclusion:PVP combined with RFA has a slight advantage in relieving pain in the short term,while PVP combined with^(125)I particle implantation may have a better effect in the relieving pain and decreasing the SSRs at 3 months after the operation.
基金supported by the National Natural Science Foundation of China(31871637,32072073,and 32001500)the Project of Transgenic New Variety Cultivation(2016ZX08003003)。
文摘The current assembled maize genomes cannot represent the broad genetic diversity of maize germplasms.Acquiring more genome sequences is critical for constructing a pan-genome and elucidating the linkage between genotype and phenotype in maize.Here we describe the genome sequence and annotation of A188,a maize inbred line with high phenotypic variation relative to other lines,acquired by single-molecule sequencing and optical genome mapping.We assembled a 2210-Mb genome with a scaffold N50 size of 11.61 million bases(Mb),compared to 9.73 Mb for B73 and 10.2 Mb for Mo17.Based on the B73_Ref Gen_V4 genome,295 scaffolds(2084.35 Mb,94.30%of the final genome assembly)were anchored and oriented on ten chromosomes.Comparative analysis revealed that~30%of the predicted A188 genes showed large structural divergence from B73,Mo17,and W22 genomes,which causes high protein divergence and may lead to phenotypic variation among the four inbred lines.As a line with high embryonic callus(EC)induction capacity,A188 provides a convenient tool for elucidating the molecular mechanism underlying the formation of EC in maize.Combining our new A188 genome with previously reported QTL and RNA sequencing data revealed eight genes with large structural variation and two differentially expressed genes playing potential roles in maize EC induction.
基金supported by National Natural Science Foundation of China (81272930, 81322038, 31260208, and U1132605)the Science and Technological Key Project of Yunnan Province (2012FB185)West Light Foundation of the Chinese Academy of Sciences (to R.L.)
文摘The WW domain-containing oxidoreductase(WWOX) is a tumor suppressor in a variety of cancers, including breast cancer. Reduced WWOX expression is associated with the basal-like subtype and a relatively poor disease-free survival rate among breast cancer patients. Though several WWOX partners have been identified, the functional mechanisms of WWOX's role in cancers have not been fully addressed to date. In the current study, we found WWOX suppresses expression of KLF5—an oncogenic transcription factor—at protein level, and suppresses cancer cell proliferation in both bladder and breast cancer cell lines. Furthermore, we demonstrated that WWOX physically interacts with KLF5 via the former's WW domains and the latter's PY motifs. Interestingly, we found the expression of WWOX negatively correlates with KLF5 expression in a panel of breast cancer cell lines. Taken together, we conjecture that WWOX may suppress cancer cell proliferation partially by reducing the expression of KLF5.
基金supported by the Construction Technology Program of the Ministry of Transport (Grant 2013 318 740050)
文摘The effects of tension on vortex-induced vibration(ⅥⅤ) responses for a tension-dominated long cylinder with an aspect ratio of 550 in uniform flows are experimentally investigated in this paper. The results show that elevated tension suppresses fluctuations of maximum displacement with respect to flow velocity and makes chaotic ⅥⅤ more likely to appear. With respect to periodic ⅥⅤ, if elevated tension is applied, the dominant vibration frequency in the in-line(IL) direction will switch from a fundamental vibration frequency to twice the value of the fundamental vibration frequency, which results in a ratio of the dominant vibration frequency in the IL direction to that in the cross-flow direction of 2.0. The suppression of the elevated tension in the fluctuation of the maximum displacement causes the axial tension to become an active control parameter for the ⅥⅤ maximum displacement of a tension-dominated long riser or tether of an engineering structure in deep oceans. However,the axial tension must be optimized before being used since the high dominant vibration frequency due to the elevated tension may unfavorably affect the fatigue life of the riser or tether.
基金supported by the National Natural Science Foundation of China(51678511,51308484)the Natural Science Foundation of Hunan Province(13JJ4049)+3 种基金the Education Department Fund of Hunan Province(14C1094)the Open Fund of Key Laboratory of Mineralogy and Metallogeny in Chinese Academy of Sciences(KLMM20150104)the Major Talent Training Program of Xiangtan University(16PYZ09)the Specialized Research Fund for the Doctoral Program of Xiangtan University(12QDZ18)~~
基金supported by the National Natural Science Foundation of China(Nos.21577117 and 22076160)the Postgraduate Scientific Research Innovation Project of Hunan Province(No.CX20210521).
文摘Adenosine triphosphate(ATP)generation of aquatic organisms is often subject to nanoparticles(NPs)stress,involving extensive reprogramming of gene expression and changes in enzyme activity accompanied by metabolic disturbances.However,little is known about the mechanism of energy supply by ATP to regulate the metabolism of aquatic organisms under NPs stress.Here,we selected extensively existing silver nanoparticles(AgNPs)to investigate their implications on ATP generation and relevant metabolic pathways in alga(Chlorella vulgaris).Results showed that ATP content significantly decreased by 94.2%of the control(without AgNPs)in the algal cells at 0.20 mg/L AgNPs,which was mainly attributed to the reduction of chloroplast ATPase activity(81.4%)and the downregulation of ATPase-coding genes atpB and atpH(74.5%-82.8%)in chloroplast.Molecular dynamics simulations demonstrated that AgNPs competed with the binding sites of substrates adenosine diphosphate and inorganic phosphate by forming a stable complex with ATPase subunit beta,potentially resulting in the reduced binding efficiency of substrates.Furthermore,metabolomics analysis proved that the ATP content positively correlated with the content of most differential metabolites such as D-talose,myo-inositol,and L-allothreonine.AgNPs remarkably inhibited ATPinvolving metabolic pathways,including inositol phosphate metabolism,phosphatidylinositol signaling system,glycerophospholipid metabolism,aminoacyl-tRNA biosynthesis,and glutathione metabolism.These results could provide a deep understanding of energy supply in regulating metabolic disturbances under NPs stress.
基金supported by the Center of Advisory,Information Research,Academic Divisions of the Chinese Academy of Sciences
文摘Purpose:This paper proposes a method of detecting emerging trends in research topics from a more micro perspective.Design/methodology/approach:Through co-word clustering to identify research topics and analyzing position changes of topic words in the keywords life-cycle diagram during different time periods,we detected emerging trends in research topics from a more micro perspective.I'he method was applied to the field of nanotechnology to verify its effectiveness and practicability.Findings:The results show that this method can be used to detect emerging trends in research topics from a more micro perspective,as it divides keywords into five categories:Potential keywords,emerging keywords,hot keywords,stable keywords and recession keywords,through which more details of topic changes can be found.Research limitations:We used keywords provided by authors and database indexers for keywords extraction.But this approach may lead to the problem of "indexer effect".The method may have a limited effect when applied to a disciplinary domain such as mathematics,which evolves slowly.Practical implications:This study provides information analysts with insights into the way to better understand specialty areas of a discipline domain and formulate research policies and strategic plans.Originality/value:This study contributes to the current literature by proposing a new method,which can detect emerging trends in research topics from a more micro perspective.
基金This work was supported by grants from the National Key Research and Development Program of China(2020YFA0112300 and 2018YFC2000400 to C.C.)the National Postdoctoral Program for Innovative Talents(BX20190088 to F.L.)+2 种基金the National Natural Science Foundation of China(82000817 to F.L.,81773149 to Y.K.,U2102203 and 81830087 to C.C.,82173014 and 81872414 to D.J.,81772847 to R.L.)the China Postdoctoral Science Foundation(2019M662869 to F.L.,182703 and 230794 to Y.K.,CAS Light of West China program(Young Scholar 2021000006 to D.J.)the Yunnan Applied Basic Research Projects(202101AS070050 to C.C.,202001AU070095 to H.L.,2018FB134 to Y.K.,2019FB112 and 202001AW070018 to D.J.).
文摘Metastasis is the leading cause of cancer-related death.The interactions between circulating tumor cells and endothelial adhesion molecules in distant organs is a key step during extravasation in hematogenous metastasis.Surgery is a common intervention for most primary solid tumors.However,surgical trauma-related systemic inflammation facilitates distant tumor metastasis by increasing the spread and adhesion of tumor cells to vascular endothelial cells(ECs).Currently,there are no effective interventions to prevent distant metastasis.Here,we show that HECTD3 deficiency in ECs significantly reduces tumor metastasis in multiple mouse models.HECTD3 depletion downregulates expression of adhesion molecules,such as VCAM-1,ICAM-1 and E-selectin,in mouse primary ECs and HUVECs stimulated by inflammatory factors and inhibits adhesion of tumor cells to ECs both in vitro and in vivo.We demonstrate that HECTD3 promotes stabilization,nuclear localization and kinase activity of IKKa by ubiquitinating IKKa with K27-and K63-linked polyubiquitin chains at K296,increasing phosphorylation of histone H3 to promote NF-kB target gene transcription.Knockout of HECTD3 in endothelium significantly inhibits tumor cells lung colonization,while conditional knockin promotes that.IKKa kinase inhibitors prevented LPS-induced pulmonary metastasis.These findings reveal the promotional role of the HECTD3-IKKa axis in tumor hematogenous metastasis and providea potential strategy for tumormetastasis prevention.
基金supported by the National Natural Science Foundation of China(No.22176161)the National Natural Science Foundation of China-Hunan Joint Fund(No.U21A20293)+2 种基金the Natural Science Foundation of Hunan Province(No.2021JJ30655)the Innovation Platform Open Fund Project of Hunan Provincial Education Committee(No.19K090)the Program of Shanghai Academic/Technology Research Leader(No.19XD1434900).
文摘This study investigated the transformations of heavy metals(HMs) and polycyclic aromatic hydrocarbons(PAHs) in rhizosphere soils and adaptive responses of rhizobacterial community under the real field conditions during four growth stages(e.g., greening, tillering, heading, and maturity) of early rice(Zhongjiazao 17) and late rice(Zhongyou 9918) in Jiangshe village(JSV) and Yangji village(YJV). Results showed that rhizosphere soils of YJV were mildly polluted by Cd and PAHs compared to that of JSV. The relative abundance of bioavailable Cd(bio-Cd) and bioavailable As(bio-As) in rhizosphere soil increased before the heading stage but decreased at the subsequent growth stage, but the content of ΣPAHs in rhizosphere soil decreased gradually during whole growth period. The dominant rhizobacteria genera at YJV(e.g., Bacillus, Massilia, Sphingomonas, and Geobacter) increased at an abundance level from the tillering to heading stage. Rhizobacteria interacted with the above co-pollutant more intensely at the tillering and heading stage, where genes involved in HM-resistance and PAH-degradation appeared to have a significant enhancement. The contents of bioCd and bio-As in rhizosphere soil of early rice were higher than that of late rice at each growth stage, especially at the heading stage. Bio-Cd, ΣPAHs, and organic matter were key factors influencing the community structure of rhizobacteria. Results of this study provide valuable insights about the interactions between HM-PAH co-pollutant and rhizobacterial community under real field conditions and thus develop in-situ rhizosphere remediation techniques for contaminated paddy fields.
基金supported by the National Natural Science Foundation of China (Nos. 21277114, and 21577117)the Natural Sciences and Engineering Research Council of Canada (NSERC)
文摘We report that green algae in lakes and rivers can serve as precursors of halobenzoquinone(HBQ) disinfection byproducts(DBPs) produced during chlorination. Chlorination of a common green alga, Chlorella vulgaris, produced 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ), the most prevalent HBQ DBP in disinfected water. Under varying p H conditions(p H 6.0–9.0), 2,6-DCBQ formation ranged from 0.3 to 2.1 μg/mg C with maximum formation at p H 8.0. To evaluate the contribution of organic components of C. vulgaris to 2,6-DCBQ formation, we separated the organics into two fractions, the protein-rich fraction of intracellular organic matter(IOM) and the polysaccharide-laden fraction of extracellular organic matter(EOM). Chlorination of IOM and EOM produced 1.4 μg/mg C and 0.7 μg/mg C of 2,6-DCBQ, respectively. The IOM generated a two-fold higher 2,6-DCBQ formation potential than the EOM fraction, suggesting that proteins are potent 2,6-DCBQ precursors. This was confirmed by the chlorination of proteins extracted from C. vulgaris: the amount of 2,6-DCBQ produced is linearly correlated with the concentration of total algal protein(R2= 0.98). These results support that proteins are the primary precursors of 2,6-DCBQ in algae, and control of green algal bloom outbreaks in source waters is important for management of HBQ DBPs.
文摘Ultrafine chitosan particles were prepared by reverse microemulsion consisting of water, Triton X-100, octanol and cyclohexane. Two methods of preparing ultrafine chitosan particles were adopted and compared using TEM and IR, and possible mechanisms for the formation of ultrafine chitosan particles were proposed. Experimental results show that the method which combined ionic gelation and cross-linking gave uniformly sized chitosan nanoparticles with an average diameter of 92 nm, while the cross-linking without ionic gelation produced spindly chitosan particles with an average length of 943 nm and width of 188 nm.
基金the National Natural Science Foundation of China(81903666 and 31930015)the Chinese Academy of Sciences(XDB31000000,KFJ-STS-SCYD-304,and K.C.Wong Education Foundation,China)+4 种基金the Science and Technology Department of Yunnan Province (202101AT070301,2019ZF003,202002AA100007,202003AD150008,and 2019FB103China)Project of Innovative Research Team of Yunnan Province(2019HC005China)the Department of Industry and Information Technology of Yunnan Province (2019-YT-053,China)。
文摘Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer cells have a higher dependency on molecular chaperones to maintain proteostasis. The chaperonin T-complex protein ring complex(TRiC) contains eight paralogous subunits(CCT1-8), and assists the folding of as many as 10% of cytosolic proteome.TRiC is essential for the progression of some cancers, but the roles of TRiC subunits in osteosarcoma remain to be explored. Here, we show that CCT4/TRiC is significantly correlated in human osteosarcoma,and plays a critical role in osteosarcoma cell survival. We identify a compound anticarin-β that can specifically bind to and inhibit CCT4. Anticarin-β shows higher selectivity in cancer cells than in normal cells. Mechanistically, anticarin-β potently impedes CCT4-mediated STAT3 maturation. Anticarin-β displays remarkable antitumor efficacy in orthotopic and patient-derived xenograft models of osteosarcoma.Collectively, our data uncover a key role of CCT4 in osteosarcoma, and propose a promising treatment strategy for osteosarcoma by disrupting CCT4 and proteostasis.
文摘Commercial vehicle industry worldwide is facing challenges from environmental pressures,stringent limits of CO_(2) emission,governmental regulations as well as ever-increased customer demands.This paper analyzes the above-mentioned challenges,especially in China,including the potential improvement to increase the brake thermal efficiency(BTE),with five levels of BTE proposed,ranging from current 45 to 60%in future,corresponding to China fuel consumption regulation(CFCR)in different phases.The authors also proposed the technology roadmaps to meet the upcoming CFCR3 and CFCR4;finally,the authors draw the conclusions to conform with ever-stringent regulation in China.
基金the National Natural Science Foundation of CN(81673795,81503536)the Natural Science Foundation of Jiangsu Province(grant no.BK20181235)+4 种基金The Medical Research Project of Jiangsu Provincial Health Commission(M2022024)Natural Science Foundation of Nanjing University of Traditional Chinese Medicine(grant no.XZR2020084)Open Project of Jiangsu Key Laboratory of Major Neuropsychiatric Diseases Research(grant no.KL20211211)the project of Jiangsu Provincial Administration of TCM(grant no.YB2017066)A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘To systematically assess the effectiveness and safety of using acupuncture with traditional Chinese medicine for functional constipation so as to provide the evidence-based data for clinical practice.Wanfang databases,CBM,VIP,CNKI,PubMed,Cochrane Library and EMbase were searched for trials based on randomization and control for use of acupuncture in combination with CHM for functional constipation published from the inception of the database to Dec.2019.We used the risk of bias of Cochrane Collaboration for literature quality assessment,extracted trial data in duplicate,and then assessed the risk of bias.The data were discussed by adopting Review Manager 5.3 program.A total of 22 RCTs with 2574 cases were retrieved.Meta-analysis showed that acupuncture with CHM could improve the clinical effectiveness(OR6.10,95%CI 4.72–7.87,P<0.00001);heterogeneity was not identified(Chi^(2)=32.84,P=0.43,I^(2)=2%),and the difference was statistically significant.Second-level outcomes(e.g.,defecation interval time(P<0.00001),Bristol Stool Scale(P=0.002),defecation difficulty(P=0.01)and accompanying symptoms(P<0.00001))in the treatment groups were significantly different after the acupuncture with CHM compared with control groups,whereas defecation symptoms were not significantly different(P=0.06).The use of acupuncture with CHM can significantly improve the clinical efficacy of functional constipation,ameliorate Bristol Stool Scale,defecation difficulty,defecation symptoms,and accompanying symptoms,as well as shorten the defecation interval time,with better safety.
基金National Natural Science Foundation of China,Grant/Award Numbers:21773168,21531005,91622111。
文摘Aggregation-induced emission(AIE)is a vital photophysical phenomenon that the luminogens in the concentrated or aggregated cases will engender the dramatically boosted emission in comparison with the dispersive states.Given this extraordinary emitting capacity exactly resolves the aggregation-caused quenching(ACQ)situations residing in the traditional luminophores,the booming AIE luminogens have drawn tremendous interest owing to their advanced performances and colossal potential applications in various areas.Further exploitations of AIE molecules also drive the research interests in the midst of these AIE materials toward the nonlinear optical(NLO)regime.The combination of AIE and NLO effects have nurtured some unforeseen properties of AIE materials and extended their application spheres.Therefore,some NLO-active AIE materials have been wielded in many crucial applications,for example,optical limiting,laser,bioimaging,and photodynamic therapy.Meanwhile,the impacts of aggregate on the NLO effect also deserve deep considerations and pursuits,and the modifications of aggregates promise an easy,efficient,and prompt avenue to tune the NLO properties of materials.The recent achievements and progress in the NLO properties of AIE materials have been summarized in this review.The second-order and third-order NLOs of the AIE materials have been introduced and their correlative applications have been discussed.