Effect of You-Gui Yin on glucocorticoid-induced apoptosis of bone marrow mesenchymal stem cells

Objective:To investigate the effect of You-Gui Yin on glucocorticoid-induced apoptosis of rat bone marrow mesenchymal stem cells and its possible mechanism.Methods:20 SD rats were divided into normal saline group and You-Gui Yin group.Ten rats in each group were given normal saline and You-Gui Yin by gavage for 2 weeks,once daily.After the gavage,the rats were sacrificed by spinal removal,blood was taken from the abdominal aorta and centrifuged to obtain blank serum and medicated serum.The rat bone marrow mesenchymal stem cells were extracted and cultured and administered in groups:blank group(10%blank serum),Model group(10%blank serum+dexamethasone 5×10-5mol/ml),traditional Chinese medicine group(10%medicated serum+dexamethasone 5×10-5mol/ml),control group(10%medicated serum),CCK-8 method was used to detect cell proliferation changes in different groups,ELISA method was used to detect bone alkaline phosphatase levels in each group,Alizarin red staining was used to compare red-stained calcium mineralized nodules in each group,Western Blot Detect the expression of apoptosis-related proteins in BMSCs,and perform molecular docking of the quantitative components to evaluate the binding strength and activity of the target and the active compound.Results:The ratio of absorbance(A450)in each time period of the model group was significantly reduced(P<0.05),and the ratio of absorbance(A450)between the traditional Chinese medicine group and the control group was significantly higher than that of the model group(P<0.05).There was no difference between the control group and the blank group(P>0.05);ELISA method showed that the ALP level of the model group was significantly lower than that of the blank group and the control group(P<0.05),the traditional Chinese medicine group significantly increased the ALP level(P<0.05),there was no significant difference between the control group and the blank group;Observation of the number of calcium mineralized nodules under alizarin red staining microscope suggests that the number of calcium mineralized nodules in the model group is significantly lower than that of each group,and there is no significant difference between the control group and the blank group.Both groups are higher than the model group and traditional Chinese medicine Group;Western Blot indicated that compared with the blank group,the expression levels of proapoptotic proteins Bax and Cleaved-casepase-3 in the model group were significantly increased(P<0.01),and the expression levels of anti-apoptotic protein Bcl-2 were decreased(P<0.01),on the contrary in the traditional Chinese medicine group,the expression of the control group did not change significantly compared with the blank group;the molecular docking results showed that You-Gui Yin mainly regulates the apoptosis of BMSCs through Epicatechin 5,7,3'-trimethyl ether,Delphinin,etc.promote bone formation.Conclusion:You-Gui Yin medicated serum can protect BMSCs from apoptosis induced by GCs and promote BMSCs proliferation and osteogenic differentiation by regulating Bax,Cleaved-caspase3,and Bcl-2.

新型杂化金属硫氰化物压电体用于能量收集和动作传感

杂化有机-无机压电材料因其电光性能优异、合成简单以及机械柔性等优点而受到广泛关注,在能源和传感领域显示出巨大的应用潜力.本文合成了一例新的零维杂化压电材料[Me-V]_(3)Mn(SCN)_(5),并与聚己二酸丁二酯对苯二甲酸丁二酯复合成膜制备了用于机械能收集的压电器件.实验结果表明,15 wt%组分的压电器件具有优异的能量收集性能,在仅0.03 MPa作用力下,可产生18.49 V的最大输出电压和7.88μW cm^(-2)的功率密度.同时,该器件在循环5500圈及60天内仍保持优异的压电性能.此外,该器件在包括手指敲击、手腕/肘部弯曲和踩踏等动作传感方面表现出良好的敏感性.这项工作证明了无铅无卤的杂化压电材料在柔性传感和能量收集方面具有巨大的应用潜力.