Waterlogging is a major abiotic stress limiting barley (Hordeum vulgare L.) yield and its stability in areas with excessive rainfall.Identification of genomic regions influencing the response of yield and its componen...Waterlogging is a major abiotic stress limiting barley (Hordeum vulgare L.) yield and its stability in areas with excessive rainfall.Identification of genomic regions influencing the response of yield and its components to waterlogging stress will enhance our understanding of the genetics of waterlogging tolerance and the development of more tolerant barley cultivars.Quantitative trait loci (QTLs) for grain yield and its components were identified using 156 doubled haploid (DH) lines derived from a cross between the cultivars Yerong (waterlogging-tolerant) and Franklin (waterlogging-sensitive) grown under different conditions (waterlogged and well drained).A total of 31 QTLs were identified for the measured characters from two experiments with two growth environments.The phenotypic variation explained by individual QTLs ranged from 4.74% to 55.34%.Several major QTLs determining kernel weight (KW),grains per spike (GS),spikes per plant (SP),spike length (SL) and grain yield (GY) were detected on the same region of chromosome 2H,indicating close linkage or pleiotropy of the gene(s) controlling these traits.Some different QTLs were identified under waterlogging conditions,and thus different markers may have to be used in selecting cultivars suitable for high rainfall areas.展开更多
High malting quality of barley (Hordeum vulgare L.) relies on many traits,such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study,interval mapping was utilized to ...High malting quality of barley (Hordeum vulgare L.) relies on many traits,such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study,interval mapping was utilized to detect quantitative trait loci (QTLs) affecting these malting quality parameters using a doubled haploid (DH) population from a cross of CM72 (six-rowed) by Gairdner (two-rowed) barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H,4H,5H,and 7H. Five of the nine QTLs mapped to chromosome 3H,indicating a possible role of loci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for β-glucan and limit dextrinase were identified on chromosomes 4H and 5H,respectively. QTL for hordeins was coincident with the region of silica eluate (SE) protein on 3HS,while QTLs for albumins,globulins,and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to β-amylase,and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.展开更多
基金Project supported by the Hi-Tech Research and Development Pro-gram (863) of China (No.2006AA10Z1C3)the Ministry of Education and the State Administration of Foreign Experts Affairs (111 Project) of China (No.B06014)the Zhejiang Provincial Department of Education Project (No.20070214),China
文摘Waterlogging is a major abiotic stress limiting barley (Hordeum vulgare L.) yield and its stability in areas with excessive rainfall.Identification of genomic regions influencing the response of yield and its components to waterlogging stress will enhance our understanding of the genetics of waterlogging tolerance and the development of more tolerant barley cultivars.Quantitative trait loci (QTLs) for grain yield and its components were identified using 156 doubled haploid (DH) lines derived from a cross between the cultivars Yerong (waterlogging-tolerant) and Franklin (waterlogging-sensitive) grown under different conditions (waterlogged and well drained).A total of 31 QTLs were identified for the measured characters from two experiments with two growth environments.The phenotypic variation explained by individual QTLs ranged from 4.74% to 55.34%.Several major QTLs determining kernel weight (KW),grains per spike (GS),spikes per plant (SP),spike length (SL) and grain yield (GY) were detected on the same region of chromosome 2H,indicating close linkage or pleiotropy of the gene(s) controlling these traits.Some different QTLs were identified under waterlogging conditions,and thus different markers may have to be used in selecting cultivars suitable for high rainfall areas.
基金Project supported by the National Natural Science Foundation of China (Nos. 30630047 and 30771281)the Hi-Tech Research and Development Program (863) of China (No. 2006AA10Z1C3)the Ministry of Education and the State Administration of Foreign Experts Affairs (111 Project) of China (No. B06014)
文摘High malting quality of barley (Hordeum vulgare L.) relies on many traits,such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study,interval mapping was utilized to detect quantitative trait loci (QTLs) affecting these malting quality parameters using a doubled haploid (DH) population from a cross of CM72 (six-rowed) by Gairdner (two-rowed) barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H,4H,5H,and 7H. Five of the nine QTLs mapped to chromosome 3H,indicating a possible role of loci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for β-glucan and limit dextrinase were identified on chromosomes 4H and 5H,respectively. QTL for hordeins was coincident with the region of silica eluate (SE) protein on 3HS,while QTLs for albumins,globulins,and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to β-amylase,and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.
