Melatonin improves synapse development by PI3K/Akt signaling in a mouse model of autism spectrum disorder

Autism spectrum disorders are a group of neurodevelopmental disorders involving more than 1100 genes,including Ctnnd2 as a candidate gene.Ctnnd2knockout mice,serving as an animal model of autis m,have been demonstrated to exhibit decreased density of dendritic spines.The role of melatonin,as a neuro hormone capable of effectively alleviating social interaction deficits and regulating the development of dendritic spines,in Ctnnd2 deletion-induced nerve injury remains unclea r.In the present study,we discove red that the deletion of exon 2 of the Ctnnd2 gene was linked to social interaction deficits,spine loss,impaired inhibitory neurons,and suppressed phosphatidylinositol-3-kinase(PI3K)/protein kinase B(Akt) signal pathway in the prefrontal cortex.Our findings demonstrated that the long-term oral administration of melatonin for 28 days effectively alleviated the aforementioned abnormalities in Ctnnd2 gene-knockout mice.Furthermore,the administration of melatonin in the prefro ntal cortex was found to improve synaptic function and activate the PI3K/Akt signal pathway in this region.The pharmacological blockade of the PI3K/Akt signal pathway with a PI3K/Akt inhibitor,wo rtmannin,and melatonin receptor antagonists,luzindole and 4-phenyl-2-propionamidotetralin,prevented the melatonin-induced enhancement of GABAergic synaptic function.These findings suggest that melatonin treatment can ameliorate GABAe rgic synaptic function by activating the PI3K/Akt signal pathway,which may contribute to the improvement of dendritic spine abnormalities in autism spectrum disorders.

新型乳化体系及其在化妆品中的应用(Ⅵ)——微乳液

微乳液(ME)是水、油、表面活性剂及助表面活性剂按照一定的比例自发形成的各向同性、热力学稳定的分散体系。微乳液粒径小,能自发形成,且具有优异的增溶作用,对增溶的成分能起到良好的保护、缓释作用的同时,还能有效促进其透皮吸收,改善其生物利用度。微乳液被认为是一种理想的活性成分的保存及输送体系,在化妆品、生物医药等诸多领域具有广阔的应用空间。基于此,本文详细介绍了微乳液及其形成机理;并简述了微乳液的制备方法,总结了影响微乳液形成及性质的关键因素;概括了微乳液的性能优势,并简单介绍了几种特殊微乳液,包括无表面活性剂微乳液、离子液体微乳液以及低共熔溶剂微乳液等。在此基础上,阐述了微乳液在化妆品领域的研究及应用现状。最后,对微乳液在化妆品领域实际应用中面临的问题及未来需要开展的研究工作提出了个人观点,希望能为微乳液在化妆品领域发挥其实际应用价值提供参考,也为性能优异的功效化妆品的开发提供依据。

Gut microbiome-based thiamine metabolism contributes to the protective effect of one acidic polysaccharide from Selaginella uncinata(Desv.)Spring against inflammatory bowel disease

Inflammatory bowel disease(IBD)is a serious disorder,and exploration of active compounds to treat it is necessary.An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata(Desv.)Spring,which contained galacturonic acid,galactose,xylose,arabinose,and rhamnose with the main chain structure of→4)-α-d-GalAp-(1→and→6)-β-d-Galp-(1→and the branched structure of→5)-α-l-Araf-(1→.Animal experiments showed that compared with Model group,SUSP-4 significantly improved body weight status,disease activity index(DAI),colonic shortening,and histopathological damage,and elevated occludin and zonula occludens protein 1(ZO-1)expression in mice induced by dextran sulfate sodium salt(DSS).16S ribosomal RNA(rRNA)sequencing indicated that SUSP-4 markedly downregulated the level of Akkermansia and Alistipes.Metabolomics results confirmed that SUSP-4 obviously elevated thiamine levels compared with Model mice by adjusting thiamine metabolism,which was further confirmed by a targeted metabolism study.Fecal transplantation experiments showed that SUSP-4 exerted an anti-IBD effect by altering the intestinal flora in mice.A mechanistic study showed that SUSP-4 markedly inhibited macrophage activation by decreasing the levels of phospho-nuclear factor kappa-B(p-NF-κB)and cyclooxygenase-2(COX-2)and elevating NF-E2-related factor 2(Nrf2)levels compared with Model group.In conclusion,SUSP-4 affected thiamine metabolism by regulating Akkermania and inhibited macrophage activation to adjust NF-κB/Nrf2/COX-2-mediated inflammation and oxidative stress against IBD.This is the first time that plant polysaccharides have been shown to affect thiamine metabolism against IBD,showing great potential for in-depth research and development applications.

Bilayer separator enabling dendrite-free zinc anode with ultralong lifespan >5000 h

Aqueous zinc(Zn)batteries with Zn metal anodes are promising clean energy storage devices with intrinsic safety and low cost.However,Zn dendrite growth severely restricts the use of Zn anodes.To effectively suppress Zn dendrite growth,we propose a bilayer separator consisting of commercial butter paper and glassfiber membrane.The dense cellulose-based butter paper(BP)with low zincophilicity and high mechanical properties prevents the pore-filling behavior of deposited Zn and related separator piercing,effectively suppressing the Zn dendrite growth.As a result,the bilayer separators endow the ZnjjZn symmetrical batteries with a superlong cycling life of Zn anodes(over 5000 h)at 0.5 mA cm^(-2) and the full batteries enhanced capacity retention,demonstrating the advancement of the bilayer separator to afford excellent cyclability of aqueous metal batteries.

反胶束刻蚀法制备具有多孔结构的高导电聚(3,4-二氧乙烯噻吩)纳米薄膜

图案化导电薄膜在传感器领域有着非常广泛的应用。本文中在亲水处理的二氧化硅晶片基板上涂覆氯化铁/PEG-PPG-PEG嵌段共聚物/正丁醇的混合溶液,形成含有反胶束纳米球的氧化剂薄膜;以3,4-二氧乙烯噻吩单体分子为气相,通过气相沉积的方式得到具有纳米孔图案的多孔性聚(3,4-二氧乙烯噻吩)(PEDOT)薄膜。利用偏光显微镜、扫描电镜研究PEDOT薄膜纳米多孔结构的形成机制,并采用四点探针测试图案化薄膜的电性能。结果表明,PEG-PPG-PEG嵌段共聚物浓度和环境湿度的增加都有助于孔的生成。在PEG-PPG-PEG质量分数0.2%和处理基片的环境湿度为60%的条件下,制备的导电图案化薄膜的电导率可达到83.68S/cm,其平均孔径可达到248nm。通过反胶束刻蚀法对导电薄膜进行图案化处理,相对于其他图案化方法操作简单、成本较低,在生物传感器、气体传感器等领域有巨大的应用潜力。

Role of dendritic cells in MYD88-mediated immune recognition and osteoinduction initiated by the implantation of biomaterials

Bone substitute material implantation has become an important treatment strategy for the repair of oral and maxillofacial bone defects.Recent studies have shown that appropriate inflammatory and immune cells are essential factors in the process of osteoinduction of bone substitute materials.Previous studies have mainly focused on innate immune cells such as macrophages.In our previous work,we found that T lymphocytes,as adaptive immune cells,are also essential in the osteoinduction procedure.As the most important antigen-presenting cell,whether dendritic cells(DCs)can recognize non-antigen biomaterials and participate in osteoinduction was still unclear.In this study,we found that surgical trauma associated with materials implantation induces necrocytosis,and this causes the release of high mobility group protein-1(HMGB1),which is adsorbed on the surface of bone substitute materials.Subsequently,HMGB1-adsorbed materials were recognized by the TLR4-MYD88-NFκB signal axis of dendritic cells,and the inflammatory response was activated.Finally,activated DCs release regeneration-related chemokines,recruit mesenchymal stem cells,and initiate the osteoinduction process.This study sheds light on the immune-regeneration process after bone substitute materials implantation,points out a potential direction for the development of bone substitute materials,and provides guidance for the development of clinical surgical methods.

基于高维特征因子的券商客户流失预警模型研究

随着证券行业的竞争日趋激烈,如何留住现有客户并预防潜在的客户流失已成为该行业管理者普遍关心的重要问题之一。本文针对证券行业客户流失进行研究。首先,结合证券行业的实际业务背景,探索了证券行业客户流失的定义;其次,提出了基于高维特征因子的独立性筛选方法;最后,基于筛选的因子,分别构建了客户流失预警日模型和周模型。研究结果表明,资产类因子和非资产类因子对预测客户流失具有显著效果,复合因子的预测效果不显著。客户流失预警日模型的外样本AUC值平均可以达到0.95以上,说明模型具有良好的预测精度。客户流失预警周模型的预测效果与日模型基本一致,并且具有计算成本低、预测效率高、模型更加稳定的特点。本文的研究结果可以为企业进行客户挽回提供策略分析,划分客户群体,针对不同流失风险的客户制定不同的挽回策略。另外,本文提出的流失预警模型在企业实际环境测试中也具有良好表现。

Deposition processes of gas hydrate-bearing sediments in the inter-canyon area of Shenhu Area in the northern South China Sea

The Shenhu Submarine Canyon Group on the northern slope of the South China Sea consists of 17 slope-confined canyons,providing a good example for investigating their hosting sediments.Three drilling sites,including W07,W18,and W19,have proven the occurrence of gas hydrate reservoirs in the inter-canyon area between canyons C11 and C12.Whereas,variations of the geomorphology and seismic facies analyzed by high-resolution 3D seismic data indicate that the gas hydrate-bearing sediments may form in different sedimentary processes.In the upper segment,a set of small-scale channels with obvious topographic lows can be identified,revealing fine-grained turbidites supplied from the shelf region during a very short-term sea-level lowstand.In the middle part,gas hydrate units at Site W07 showing mounded or undulation external configuration are interpreted as sliding sedimentary features,and those features caused by gravity destabilization were the main formative mechanism of gas hydrate-bearing sediments that were sourced from the upper segments.In contrast,for the canyon transition zone of lower segments between C11-C12 inter-canyon and C12 intra-canyon areas,where W18 and W19 sites are located,the gas hydratebearing sediments are deposited in the channelized feature in the middle to lower segment and slide erosive surface.Gas hydrate-bearing sediments of the lower segment were migrated through channelized features interconnecting with the middle to lower slope by gravity-driven flows.The majority of deposits tended to be furtherly moved by lateral migration via erosive surface created by sediment failed to intra-canyon area.The conclusion of this study may help better understand the interaction between the formation mechanism of gas hydrate-bearing sediments and the geomorphologic effects of inter-canyon areas.

Emergency Material Reserve Location Research in Urban Areas Based on the Supply and Demand Perspective: A Case from China

In order to improve the scientific layout of urban emergency material reserve, first, considering that the road condition has an important impact on the effect of emergency rescue, the concept of road damage risk factor is introduced in this article to build satisfaction attenuation function based on the thought of demand satisfaction decreasing within the coverage radius;second, based on the maximum coverage model, a multi-objective and multi-repeated cover model was established to solve the limited supply capacity of emergency supplies reserve;finally, the model is verified by using Yanqing district in Beijing. The study’s results showed that the improved maximum coverage model can scientifically determine the coverage and quantity of emergency supplies of each emergency material reserve, and improve the efficiency and scientificity of emergency supplies distribution. In order to avoid the disorderly distribution of emergency supplies, it is suggested that the coverage radius and scope of the emergency material reserve should be determined based on the balance between supply and demand.

A GPCR/secretase complex regulates β- and γ-secretase specificity for Aβ production and contributes to AD pathogenesis

Dysregulation of β-site APP-cleaving enzyme （BACE） and/or γ-secretase leads to anomalous production of amyloid-β peptide （Aβ） and contributes to the etiology of Alzheimer＇s disease （AD）. Since these secretases mediate proteolytic processing of numerous proteins, little success has been achieved to treat AD by secretase inhibitors because of inevitable undesired side effects. Thus, it is of importance to unravel the regulatory mechanisms of these secretases. Here, we show that δ-opioid receptor （DOR） promotes the processing of Aβ precursor protein （APP） by BACE1 and γ-secretase, but not that of Notch, N-cadherin or APLP. Further investigation reveals that DOR forms a complex with BACE1 and γ-seeretase, and activation of DOR mediates the co-endocytic sorting of the secretases/ receptor complex for APP endoproteolysis. Dysfunction of the receptor retards the endocytosis of BACE1 and γ-secretase and thus the production of Aβ Consistently, knockdown or antagonization of DOR reduces secretase activities and ameliorates Aβ pathology and Aβ-dependent behavioral deficits, but does not affect the processing of Notch, N-cadherin or APLP in AD model mice. Our study not only uncovers a molecular mechanism for the formation of a DOR/secretase complex that regulates the specificity of secretase for Aβ production but also suggests that intervention of either formation or trafficking of the GPCR/secretase complex could lead to a new strategy against AD, potentially with fewer side effects.

Metal-Organic Framework-Based Sulfur-Loaded Materials

Lithium-sulfur batteries(LSBs)are considered promising new energy storage systems given their outstanding theoretical energy densities.Nevertheless,issues such as low electrical conductivity and severe volume expansion,along with the formation of polysulfides during cycling,restrict their practical applications.To overcome these issues,it is necessary to find suitable and effective sulfur host materials.Metal-organic frameworks(MOFs),which are porous crystalline materials in the bourgeoning developmental stages,have demonstrated enormous potential in LSBs owing to their high porosity and tunable porous structure.Herein,we provide a comprehensive overview of MOF-based sulfur-loaded materials and discuss the charge/discharge mechanisms,strategies of enhancing battery performance,sulfur loading methods,and applications in LSBs.An outlook on future directions,prospects,and possible obstacles for the development of these materials is also provided.

Development and validation of InDel markers for identification of QTL underlying flowering time in soybean

Soybean [Glycine max(L.) Merrill] is a major plant source of protein and oil. An accurate and well-saturated molecular linkage map is a prerequisite for forward genetic studies of gene function and for modern breeding for many useful agronomic traits. Next-generation sequence data available in public databases provides valuable information and offers new insights for rapid and efficient development of molecular markers. In this study, we attempted to show the feasibility and facility of using genomic resequencing data as raw material for identifying putative In Del markers. First, we identified 17,613 In Del sites among 56 soybean accessions and obtained 12,619 primer pairs. Second, we constructed a genetic map with a random subset of 2841 primer pairs and aligned 300 polymorphic markers with the 20 consensus linkage groups(LG). The total genetic distance was 2347.3 c M and the number of mapped markers per LG ranged from 10 to 23 with an average of 15 markers. The largest and smallest genetic distances between adjacent markers were 52.3 c M and 0.1 cM, respectively. Finally, we validated the genetic map constructed by newly developed In Del markers by QTL analysis of days to flowering(DTF) under different environments. One major QTL(qDTF4) and four minor QTL(qDTF20, qDTF13, qDTF12,and q DTF11) on 5 LGs were detected. These results demonstrate the utility of the In Del markers developed in this work for map-based cloning and molecular breeding in soybean.

Pressure Driven Structural Evolutions of 0.935(Na0.5Bi0.5)TiO_(3)-0.065BaTiO_(3)Lead-Free Ferroelectric Single Crystal through Raman Spectroscopy

Pressure evolution of local structure and vibrational dynamics of the perovskite-type relaxor ferroelectric single crystal of 0.935(Na0.5Bi0.5)TiO3-0.065BaTiO3(NBT-6.5BT)is systematically investigated via in situ Raman spectroscopy.The pressure dependence of phonon modes up to 30GPa reveals two characteristic pressures:one is at around 4.6GPa which corresponds to the rhombohedral-to-tetragonal phase transition,showing that the pressure strongly suppresses the coupling between the off-centered A-and B-site cations;the other structural transition involving the oxygen octahedral tilt and vibration occurs at pressure∼13–15GPa with certain degree of order-disorder transition,evidenced by the abnormal changes of intensity and FWHM in Raman spectrum.

Improving chemical and sensory quality of high-oleic peanut by application of foliar fertilizer

High-oleic peanut is favored by consumers because its a longer shelf life and benefits for cardiovascular health.Up to the high-oleic standard and with good taste is essential for peanut products in food processing.The aim of present study was to explore the effect of foliar fertilizers on chemical and eating quality of high-oleic peanuts.Three high-oleic peanut cultivars,namely Huayu 09C2,Huayu 9621,and Huayu 962,were used as materials.Huayu 09C2 and Huayu 9621 were planted in Fuxin,Liaoning,foliar sprayed with Fosforil at 2250 mL/ha,and Huayu 962 was sown in Jvnan,Shandong,foliar sprayed with Foliplus at 1500/3000 mL/ha.The chemical and sensory quality of peanut harvested from the treatment plots and controls was evaluated.Results showed that,Huayu 09C2 and Huayu 9621,foliar spraying with Fosforil in Fuxin,Huayu 962,foliar spraying with Foliplus in Jvnan,all still maintained high oleate content levels,and the Fosforil treatment was effective in raising the intensity of sweetness of roasted kernels.This study demonstrated the feasibility of improving chemical and sensory quality of high-oleic peanut through foliar fertilizer.

Effect of variety and seed dressing on emergence of high-oleic peanut under low temperature and high soil humidity conditions

Low temperature coupled with high soil moisture during sowing to emergence generally results in poor peanut stand,thereby posing a non-negligible threat to peanut production in north and northeast China.Five high-oleic(HO)peanut cultivars and 4 seed dressing treatments capable of controlling several diseases and insect pests along with untreated checks were used to find the best combinations to cope with the dual stresses during sowing to emergence period.High broad-sense hereditability estimates of seedling emergence indicated great potential for genetic improvement of this trait.Analysis of variance in the split-plot experiment showed that the main effects of variety and seed dressing and their interaction on seedling emergence were significant.Seed dressing treatments increased seedling emergence percentage by 2.09–35.00 percent points.Four of the 5 HO peanut cultivars yielded satisfactory results.For Huayu 665,Huayu 668 and Huayu 965,Huweisanbao may be the best option;for Huayu 962,Weilidan was highly acceptable.These combinations will be evaluated further in multiple environments before large-scale extension.

Fractional-Order Modeling and Dynamical Analysis of a Francis Hydro-Turbine Governing System with Complex Penstocks

This paper investigates the stability of the Francis hydro-turbine governing system with complex penstocks in the grid-connected mode. Firstly, a novel fractional-order nonlinear mathematical model of a Francis hydro-turbine governing system with complex penstocks is built from an engineering application perspective. This model is described by state-space equations and is composed of the Francis hydro-turbine model, the fractional-order complex penstocks model, the third-order generator model, and the hydraulic speed governing system model. Based on stability theory for a fractional-order nonlinear system, this study discovers a basic law of the bifurcation points of the above system with a change in the fractional-order a. Secondly, the stable region of the governing system is investigated in detail,and nonlinear dynamical behaviors of the system are identified and studied exhaustively via bifurcation diagrams, time waveforms, phase orbits, Poincare maps, power spectrums and spectrograms. Results of these numerical experiments provide a theoretical reference for further studies of the stability of hydropower stations.

阴虚内热的临床研究概述

本文对近年来有关阴虚内热的临床研究进展进行了综述,希望能够对读者认识及探讨有关阴虚内热方面的问题有所帮助。

Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley

Leaf development underlies crop growth and productivity and has been a major target of crop domestication and improvement.However,most genes controlling leaf development in barley remain unknown.We identified a dwarf and liguleless(dl)mutant derived by ethylmethane sulfonate mutagenesis.The dl mutant showed dramatic changes in shoot architecture compared with wild-type(Yangnongpi 5)plants.Besides lacking ligules,the dl mutant showed much shorter plant height(28 cm)than Yangnongpi 5(78 cm).By map-based cloning,the dl gene was localized to a 56.58-kb genomic interval on the long arm of chromosome 7.A C-to-T single-nucleotide substitution was identified at exon position 790,and is a functional mutation resulting in a proline-to-serine substitution at the 264th amino acid residue of HORVU7Hr1G106960.Consequently,HORVU7Hr1G106960 was identified as the DL gene,encoding 269 amino acids and containing the Arabidopsis LSH1 and Oryza G1(ALOG)domain.DL is highly similar to rice OsG1-LIKE 1/2(OsG1L1/2)and sorghum AWN1/AWN1-10 at the amino acid level.Although the dl mutant allele showed no expression changes in selected tissues by real-time PCR,we propose HORVU7Hr1G106960 as a candidate gene conferring the dwarf and liguleless phenotype in barley.

Solvent Effects on Preparation of Pd-Based Catalysts: Influence on Properties of Palladium and Its Catalytic Activity for Benzyl Alcohol Oxidation

Solvent effects are important for the solution preparation of nanoscale metal materials, in which the sizes and properties of the nanomaterials are greatly influenced by the solvents. In this study, a series of Pd/XC-72 catalysts were synthesized by a facile solvothermal method in solvents with different numbers of hydroxyl groups, including ethanol, ethylene glycol, and glycerine. The as-prepared catalysts Pd/XC-72(EA), Pd/XC-72(EG) and Pd/XC-72(GI) were characterized by XRD, TEM, TGA, ICP-MS and multipoint nitrogen adsorption-desorption experiments. It was found that Pd/XC-72(GI) had the highest metallic dispersion and the smallest particle size (4.9 nm). Furthermore, Pd/XC-72(GI) exhibited the best catalytic performance for solvent-free selective oxidation of benzyl alcohol while the catalytic activities of Pd/XC-72 catalysts decreased in the order: Pd/XC-72(GI) > Pd/XC-72(EG) > Pd/XC-72(EA).